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. 1987 May;31(5):794–798. doi: 10.1128/aac.31.5.794

Quantitative neutralization assay of fungicidal activity of disinfectants.

B Terleckyj, D A Axler
PMCID: PMC174835  PMID: 3300541

Abstract

A quantitative assay using a neutralization medium was developed to evaluate fungicidal activity of disinfectants. Concentrated Dey-Engley neutralizing broth was used in this study and was demonstrated to inactivate various chemical agents within 5 min when disinfectant concentrations were reduced to specific levels. Addition of this Dey-Engley broth to test tubes containing fungal cells and disinfectants permitted control of the various interactions times. Subsequent concentration of the disinfectant-treated cells to a 1-ml final volume also permitted examination of a larger population for the presence of resistant cells. After timed exposures of 15, 30, and 60 min, only three of seven disinfectant solutions were found to be lethal for quantitative populations of 11 fungi tested. The recommended use dilution formulations of a quaternary ammonium product and an iodophor product were the least effective agents. Various fungi, including Trichophyton mentagrophytes, Epidermophyton floccosum, and Aspergillus niger, survived 30- to 60-min interactions with these disinfectant solutions. The most resistant organism encountered was Aspergillus fumigatus, which survived 60 and even 90 min of exposure to most disinfectants. Only use dilutions of a chlorine dioxide formulation, a glutaraldehyde formulation, and an ethyl alcohol product were effective against this species and all of the other fungi after a 15-min interaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amonette R. A., Rosenberg E. W. Infection of toe webs by gram-negative bacteria. Arch Dermatol. 1973 Jan;107(1):71–73. [PubMed] [Google Scholar]
  2. Anderson R. L., Berkelman R. L., Mackel D. C., Davis B. J., Holland B. W., Martone W. J. Investigations into the survival of Pseudomonas aeruginosa in poloxamer-iodine. Appl Environ Microbiol. 1984 Apr;47(4):757–762. doi: 10.1128/aem.47.4.757-762.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Christensen E. A., Jepsen O. B., Kristensen H., Steen G. In-use tests of disinfectants. Acta Pathol Microbiol Immunol Scand B. 1982 Apr;90(2):95–100. doi: 10.1111/j.1699-0463.1982.tb00088.x. [DOI] [PubMed] [Google Scholar]
  4. Craven D. E., Moody B., Connolly M. G., Kollisch N. R., Stottmeier K. D., McCabe W. R. Pseudobacteremia caused by povidone-iodine solution contaminated with Pseudomonas cepacia. N Engl J Med. 1981 Sep 10;305(11):621–623. doi: 10.1056/NEJM198109103051106. [DOI] [PubMed] [Google Scholar]
  5. Dixon R. E., Kaslow R. A., Mackel D. C., Fulkerson C. C., Mallison G. F. Aqueous quaternary ammonium antiseptics and disinfectants. Use and misuse. JAMA. 1976 Nov 22;236(21):2415–2417. [PubMed] [Google Scholar]
  6. Ehrenkranz N. J., Bolyard E. A., Wiener M., Cleary T. J. Antibiotic-sensitive Serratia marcescens infections complicating cardiopulmonary operations: contaminated disinfectant as a reservoir. Lancet. 1980 Dec 13;2(8207):1289–1292. doi: 10.1016/s0140-6736(80)92349-1. [DOI] [PubMed] [Google Scholar]
  7. Frank M. J., Schaffner W. Contaminated aqueous benzalkonium chloride. An unnecessary hospital infection hazard. JAMA. 1976 Nov 22;236(21):2418–2419. [PubMed] [Google Scholar]
  8. Newman K. A., Tenney J. H., Oken H. A., Moody M. R., Wharton R., Schimpff S. C. Persistent isolation of an unusual Pseudomonas species from a phenolic disinfectant system. Infect Control. 1984 May;5(5):219–222. doi: 10.1017/s0195941700060148. [DOI] [PubMed] [Google Scholar]
  9. Parrott P. L., Terry P. M., Whitworth E. N., Frawley L. W., Coble R. S., Wachsmuth I. K., McGowan J. E., Jr Pseudomonas aeruginosa peritonitis associated with contaminated poloxamer-iodine solution. Lancet. 1982 Sep 25;2(8300):683–685. doi: 10.1016/s0140-6736(82)90712-7. [DOI] [PubMed] [Google Scholar]
  10. Rutala W. A., Cole E. C. Antiseptics and disinfectants--safe and effective? Infect Control. 1984 May;5(5):215–218. doi: 10.1017/s0195941700060136. [DOI] [PubMed] [Google Scholar]
  11. Sautter R. L., Mattman L. H., Legaspi R. C. Serratia marcescens meningitis associated with a contaminated benzalkonium chloride solution. Infect Control. 1984 May;5(5):223–225. doi: 10.1017/s019594170006015x. [DOI] [PubMed] [Google Scholar]
  12. Stewart R. C., Ayers L. W. A plea for more rationaluse of "cold sterilization" procedures in Podiatric medicine. J Am Podiatry Assoc. 1977 Jun;67(6):441–444. doi: 10.7547/87507315-67-6-441. [DOI] [PubMed] [Google Scholar]
  13. Tachibana D. K. Microbiology of the foot. Annu Rev Microbiol. 1976;30:351–375. doi: 10.1146/annurev.mi.30.100176.002031. [DOI] [PubMed] [Google Scholar]
  14. van de Voorde H., Reybrouck G., van Dijck P., Vranckx M. J. The choice of fungi as test organisms in disinfectant testing. Zentralbl Bakteriol Mikrobiol Hyg B. 1984 May;179(2):125–129. [PubMed] [Google Scholar]

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