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. 1987 Jul;31(7):982–985. doi: 10.1128/aac.31.7.982

Pharmacokinetics and therapeutic efficacy of gentamicin in an experimental pleural empyema rabbit model.

I Shohet 1, A Yellin 1, J Meyerovitch 1, E Rubinstein 1
PMCID: PMC174856  PMID: 3116920

Abstract

The pharmacokinetics and therapeutic efficacy of gentamicin were investigated in an experimental pleural empyema rabbit model. Pleural effusion was induced by the intrapleural administration of turpentine, and empyema was induced by direct inoculation of the effusion with Klebsiella pneumoniae. Pleural empyema compared with effusion was characterized by lower pH, oxygen tension (PaO2), and glucose levels and higher leukocyte count, lactic acid concentration, and PaCO2. After a single administration, gentamicin was first detectable in the pleural fluid at 60 min, whereas peak levels in empyema were observed at 180 min. Gentamicin persisted in the empyema longer than in blood. Animals treated with gentamicin only had 60% bacterial cure on day 7; those treated with gentamicin in an oxygen chamber had 100% cure on day 5 (P = 0.004). Low oxygen tension diminished the antibacterial efficacy of gentamicin in this model. An increase in oxygen tension improved the therapeutic results without alteration of the pharmacokinetics of gentamicin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergan T. Pharmacokinetics of tissue penetration of antibiotics. Rev Infect Dis. 1981 Jan-Feb;3(1):45–66. doi: 10.1093/clinids/3.1.45. [DOI] [PubMed] [Google Scholar]
  2. Brook I. Measurement of lactic acid in pleural fluid. Respiration. 1980;40(6):344–348. doi: 10.1159/000194303. [DOI] [PubMed] [Google Scholar]
  3. Bryant R. E., Hammond D. Interaction of purulent material with antibiotics used to treat Pseudomonas infections. Antimicrob Agents Chemother. 1974 Dec;6(6):702–707. doi: 10.1128/aac.6.6.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirsch A., Ruffie P., Nebut M., Bignon J., Chrétien J. Pleural effusion: laboratory tests in 300 cases. Thorax. 1979 Feb;34(1):106–112. doi: 10.1136/thx.34.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Howe C., Sampath A., Spotnitz M. The pseudomallei group: a review. J Infect Dis. 1971 Dec;124(6):598–606. doi: 10.1093/infdis/124.6.598. [DOI] [PubMed] [Google Scholar]
  6. Lew P. D., Zubler R., Vaudaux P., Farquet J. J., Waldvogel F. A., Lambert P. H. Decreased heat-labile opsonic activity and complement levels associated with evidence of C3 breakdown products in infected pleural effusions. J Clin Invest. 1979 Feb;63(2):326–334. doi: 10.1172/JCI109306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McCune R. DELIVERY OF ANTIMICROBIAL DRUGS ACROSS INFLAMMATORY MEMBRANE IN RABBITS. J Clin Invest. 1960 Jun;39(6):846–853. doi: 10.1172/JCI104105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Menkin V. Studies on Inflammation: X. The Cytological Picture of an Inflammatory Exudate in Relation to its Hydrogen Ion Concentration. Am J Pathol. 1934 Mar;10(2):193–210. [PMC free article] [PubMed] [Google Scholar]
  9. Morin J. E., Munro D. D., MacLean L. D. Early thoracotomy for empyema. J Thorac Cardiovasc Surg. 1972 Oct;64(4):530–536. [PubMed] [Google Scholar]
  10. Nichols R. L., Smith J. W., Fossedal E. N., Condon R. E. Efficacy of parenteral antibiotics in the treatment of experimentally induced intraabdominal sepsis. Rev Infect Dis. 1979 Mar-Apr;1(2):302–312. doi: 10.1093/clinids/1.2.302. [DOI] [PubMed] [Google Scholar]
  11. Potts D. E., Levin D. C., Sahn S. A. Pleural fluid pH in parapneumonic effusions. Chest. 1976 Sep;70(03):328–331. doi: 10.1378/chest.70.3.328. [DOI] [PubMed] [Google Scholar]
  12. Ristuccia A. M., Cunha B. A. The aminoglycosides. Med Clin North Am. 1982 Jan;66(1):303–312. doi: 10.1016/s0025-7125(16)31462-6. [DOI] [PubMed] [Google Scholar]
  13. Rubinstein E., Dreznik Z., Mark Z. Gentamicin and cefsulodin efficacy in a rat abscess model. Surg Gynecol Obstet. 1982 Sep;155(3):363–368. [PubMed] [Google Scholar]
  14. SPECTOR W. G., WILLOUGHBY D. A. The demonstration of the role of mediators in turpentine pleurisy in rats by experimental suppression of the inflammatory changes. J Pathol Bacteriol. 1959 Jan;77(1):1–17. [PubMed] [Google Scholar]
  15. Sahn S. A., Potts D. E. Turpentine pleurisy in rabbits: a model of pleural fluid acidosis and low pleural fluid glucose. Am Rev Respir Dis. 1978 Nov;118(5):893–901. doi: 10.1164/arrd.1978.118.5.893. [DOI] [PubMed] [Google Scholar]
  16. Sahn S. A., Taryle D. A., Good J. T., Jr Experimental empyema. Time course and pathogenesis of pleural fluid acidosis and low pleural fluid glucose. Am Rev Respir Dis. 1979 Aug;120(2):355–361. doi: 10.1164/arrd.1979.120.2.355. [DOI] [PubMed] [Google Scholar]
  17. Sullivan K. M., O'Toole R. D., Fisher R. H., Sullivan K. N. Anaerobic empyema thoracis. The role of anaerobes in 226 cases of culture-proven empyemas. Arch Intern Med. 1973 Apr;131(4):521–527. doi: 10.1001/archinte.131.4.521. [DOI] [PubMed] [Google Scholar]
  18. Suter S., Nydegger U. E., Roux L., Waldvogel F. A. Cleavage of C3 by neutral proteases from granulocytes in pleural empyema. J Infect Dis. 1981 Dec;144(6):499–508. doi: 10.1093/infdis/144.6.499. [DOI] [PubMed] [Google Scholar]
  19. Van Etta L. L., Peterson L. R., Fasching C. E., Gerding D. N. Effect of the ratio of surface area to volume on the penetration of antibiotics in to extravascular spaces in an in vitro model. J Infect Dis. 1982 Sep;146(3):423–428. doi: 10.1093/infdis/146.3.423. [DOI] [PubMed] [Google Scholar]
  20. Varkey B., Rose H. D., Kutty C. P., Politis J. Empyema thoracis during a ten-year period. Analysis of 72 cases and comparison to a previous study (1952 to 1967). Arch Intern Med. 1981 Dec;141(13):1771–1776. doi: 10.1001/archinte.141.13.1771. [DOI] [PubMed] [Google Scholar]
  21. Weese W. C., Shindler E. R., Smith I. M., Rabinovich S. Empyema of the thorax then and now. A study of 122 cases over four decades. Arch Intern Med. 1973 Apr;131(4):516–520. [PubMed] [Google Scholar]
  22. Weinstein W. M., Onderdonk A. B., Bartlett J. G., Louie T. J., Gorbach S. L. Antimicrobial therapy of experimental intraabdominal sepsis. J Infect Dis. 1975 Sep;132(3):282–286. doi: 10.1093/infdis/132.3.282. [DOI] [PubMed] [Google Scholar]

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