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. 1987 Aug;31(8):1222–1224. doi: 10.1128/aac.31.8.1222

Determination of teicoplanin concentrations in serum by high-pressure liquid chromatography.

B Joos, R Lüthy
PMCID: PMC174907  PMID: 2957953

Abstract

An isocratic reversed-phase high-pressure liquid chromatographic method for the determination of six components of the teicoplanin complex in biological fluid was developed. By using fluorescence detection after precolumn derivatization with fluorescamine, the assay is specific and highly sensitive, with reproducibility studies yielding coefficients of variation ranging from 1.5 to 8.5% (at 5 to 80 micrograms/ml). Response was linear from 2.5 to 80 micrograms/ml (r = 0.999); the recovery from spiked human serum was 76%. An external quality control was performed to compare this high-pressure liquid chromatographic method (H) with a standard microbiological assay (M); no significant deviation from slope = 1 and intercept = 0 was found by regression analysis (H = 1.03M - 0.45; n = 15).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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