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. 1987 Nov;31(11):1852–1854. doi: 10.1128/aac.31.11.1852

Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain.

J T Brown 1, M C Roberts 1
PMCID: PMC175053  PMID: 3435130

Abstract

We have cloned a 4.9-kilobase (kb) HincII fragment which contains a tetracycline resistance determinant (tetM) from the chromosome of Ureaplasma urealyticum. The 4.9-kb HincII fragment contains DNA in addition to the structural gene, is closely related to the previously characterized 5.0-kb fragment from pJI3, and contains no normal ureaplasmal DNA sequences.

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Selected References

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  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  3. Burdett V. Identification of tetracycline-resistant R-plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1980 Nov;18(5):753–760. doi: 10.1128/aac.18.5.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burdett V., Inamine J., Rajagopalan S. Heterogeneity of tetracycline resistance determinants in Streptococcus. J Bacteriol. 1982 Mar;149(3):995–1004. doi: 10.1128/jb.149.3.995-1004.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burdett V. Streptococcal tetracycline resistance mediated at the level of protein synthesis. J Bacteriol. 1986 Feb;165(2):564–569. doi: 10.1128/jb.165.2.564-569.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Buu-Hoï A., Horodniceanu T. Conjugative transfer of multiple antibiotic resistance markers in Streptococcus pneumoniae. J Bacteriol. 1980 Jul;143(1):313–320. doi: 10.1128/jb.143.1.313-320.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clewell D. B., Gawron-Burke C. Conjugative transposons and the dissemination of antibiotic resistance in streptococci. Annu Rev Microbiol. 1986;40:635–659. doi: 10.1146/annurev.mi.40.100186.003223. [DOI] [PubMed] [Google Scholar]
  9. Dybvig K., Cassell G. H. Transposition of gram-positive transposon Tn916 in Acholeplasma laidlawii and Mycoplasma pulmonis. Science. 1987 Mar 13;235(4794):1392–1394. doi: 10.1126/science.3029869. [DOI] [PubMed] [Google Scholar]
  10. Ford D. K., Smith J. R. Non-specific urethritis associated with a tetracycline-resistant T-mycoplasma. Br J Vener Dis. 1974 Oct;50(5):373–374. doi: 10.1136/sti.50.5.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Franke A. E., Clewell D. B. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. J Bacteriol. 1981 Jan;145(1):494–502. doi: 10.1128/jb.145.1.494-502.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  13. Horodniceanu T., Bougueleret L., El-Solh N., Bouanchaud D. H., Chabbert Y. A. Conjugative R plasmids in Streptococcus agalactiae (group B). Plasmid. 1979 Apr;2(2):197–206. doi: 10.1016/0147-619x(79)90038-6. [DOI] [PubMed] [Google Scholar]
  14. Hächler H., Kayser F. H., Berger-Bächi B. Homology of a transferable tetracycline resistance determinant of Clostridium difficile with Streptococcus (Enterococcus) faecalis transposon Tn916. Antimicrob Agents Chemother. 1987 Jul;31(7):1033–1038. doi: 10.1128/aac.31.7.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Inamine J. M., Burdett V. Structural organization of a 67-kilobase streptococcal conjugative element mediating multiple antibiotic resistance. J Bacteriol. 1985 Feb;161(2):620–626. doi: 10.1128/jb.161.2.620-626.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marshall B., Tachibana C., Levy S. B. Frequency of tetracycline resistance determinant classes among lactose-fermenting coliforms. Antimicrob Agents Chemother. 1983 Dec;24(6):835–840. doi: 10.1128/aac.24.6.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Martin P., Trieu-Cuot P., Courvalin P. Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545. Nucleic Acids Res. 1986 Sep 11;14(17):7047–7058. doi: 10.1093/nar/14.17.7047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McMurry L., Petrucci R. E., Jr, Levy S. B. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974–3977. doi: 10.1073/pnas.77.7.3974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mendez B., Tachibana C., Levy S. B. Heterogeneity of tetracycline resistance determinants. Plasmid. 1980 Mar;3(2):99–108. doi: 10.1016/0147-619x(80)90101-8. [DOI] [PubMed] [Google Scholar]
  20. Morse S. A., Johnson S. R., Biddle J. W., Roberts M. C. High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant. Antimicrob Agents Chemother. 1986 Nov;30(5):664–670. doi: 10.1128/aac.30.5.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Roberts M. C., Hillier S. L., Hale J., Holmes K. K., Kenny G. E. Tetracycline resistance and tetM in pathogenic urogenital bacteria. Antimicrob Agents Chemother. 1986 Nov;30(5):810–812. doi: 10.1128/aac.30.5.810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roberts M. C., Kenny G. E. Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis. J Bacteriol. 1987 Aug;169(8):3836–3839. doi: 10.1128/jb.169.8.3836-3839.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Roberts M. C., Kenny G. E. Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum. Antimicrob Agents Chemother. 1986 Feb;29(2):350–352. doi: 10.1128/aac.29.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Roberts M. C., Kenny G. E. TetM tetracycline-resistant determinants in Ureaplasma urealyticum. Pediatr Infect Dis. 1986 Nov-Dec;5(6 Suppl):S338–S340. doi: 10.1097/00006454-198611010-00032. [DOI] [PubMed] [Google Scholar]
  25. Roberts M. C., Koutsky L. A., Holmes K. K., LeBlanc D. J., Kenny G. E. Tetracycline-resistant Mycoplasma hominis strains contain streptococcal tetM sequences. Antimicrob Agents Chemother. 1985 Jul;28(1):141–143. doi: 10.1128/aac.28.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  27. Trieu-Cuot P., Gerbaud G., Lambert T., Courvalin P. In vivo transfer of genetic information between gram-positive and gram-negative bacteria. EMBO J. 1985 Dec 16;4(13A):3583–3587. doi: 10.1002/j.1460-2075.1985.tb04120.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  29. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]

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