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. 1997 Mar;65(3):1095–1097. doi: 10.1128/iai.65.3.1095-1097.1997

Involvement of tubulin and inhibitory G proteins in the interaction of Listeria monocytogenes with mouse hepatocytes.

I B Buchwalow 1, M Emoto 1, M Brich 1, S H Kaufmann 1
PMCID: PMC175093  PMID: 9038321

Abstract

Intracellular and cell-to-cell spread of Listeria monocytogenes has been considered exclusively actin dependent. By immunocytochemical techniques, we provide evidence for an involvement of inhibitory G proteins and tubulin in "comet tail" formation in L. monocytogenes-infected mouse hepatocytes.

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Selected References

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  1. Bermudes D., Hinkle G., Margulis L. Do prokaryotes contain microtubules? Microbiol Rev. 1994 Sep;58(3):387–400. doi: 10.1128/mr.58.3.387-400.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bermudez L. E., Goodman J. Mycobacterium tuberculosis invades and replicates within type II alveolar cells. Infect Immun. 1996 Apr;64(4):1400–1406. doi: 10.1128/iai.64.4.1400-1406.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Desjardins M. Biogenesis of phagolysosomes: the 'kiss and run' hypothesis. Trends Cell Biol. 1995 May;5(5):183–186. doi: 10.1016/s0962-8924(00)88989-8. [DOI] [PubMed] [Google Scholar]
  4. Domann E., Wehland J., Rohde M., Pistor S., Hartl M., Goebel W., Leimeister-Wächter M., Wuenscher M., Chakraborty T. A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin. EMBO J. 1992 May;11(5):1981–1990. doi: 10.1002/j.1460-2075.1992.tb05252.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guzman C. A., Rohde M., Chakraborty T., Domann E., Hudel M., Wehland J., Timmis K. N. Interaction of Listeria monocytogenes with mouse dendritic cells. Infect Immun. 1995 Sep;63(9):3665–3673. doi: 10.1128/iai.63.9.3665-3673.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hamilton T. A., Ohmori Y., Tebo J. M., Narumi S., Tannenbaum C. S. Transmembrane and intracellular signaling events in lipopolysaccharide-stimulated macrophages. Immunol Ser. 1994;60:83–97. [PubMed] [Google Scholar]
  7. Kocks C., Gouin E., Tabouret M., Berche P., Ohayon H., Cossart P. L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein. Cell. 1992 Feb 7;68(3):521–531. doi: 10.1016/0092-8674(92)90188-i. [DOI] [PubMed] [Google Scholar]
  8. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mounier J., Ryter A., Coquis-Rondon M., Sansonetti P. J. Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enterocytelike cell line Caco-2. Infect Immun. 1990 Apr;58(4):1048–1058. doi: 10.1128/iai.58.4.1048-1058.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oelschlaeger T. A., Guerry P., Kopecko D. J. Unusual microtubule-dependent endocytosis mechanisms triggered by Campylobacter jejuni and Citrobacter freundii. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6884–6888. doi: 10.1073/pnas.90.14.6884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Parton R. G. Endocytosis in polarized cells. Semin Cell Biol. 1991 Dec;2(6):387–395. [PubMed] [Google Scholar]
  12. Roychowdhury S., Rasenick M. M. Tubulin-G protein association stabilizes GTP binding and activates GTPase: cytoskeletal participation in neuronal signal transduction. Biochemistry. 1994 Aug 16;33(32):9800–9805. doi: 10.1021/bi00198a052. [DOI] [PubMed] [Google Scholar]
  13. Schulze W., Buchwalow I. B., Wolf W. P., Will-Shahab L. Comparative immunocytochemical demonstration of G proteins in rat heart tissue. Acta Histochem. 1994 Mar;96(1):87–95. doi: 10.1016/S0065-1281(11)80014-0. [DOI] [PubMed] [Google Scholar]
  14. Szalay G., Hess J., Kaufmann S. H. Restricted replication of Listeria monocytogenes in a gamma interferon-activated murine hepatocyte line. Infect Immun. 1995 Aug;63(8):3187–3195. doi: 10.1128/iai.63.8.3187-3195.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tilney L. G., Portnoy D. A. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. J Cell Biol. 1989 Oct;109(4 Pt 1):1597–1608. doi: 10.1083/jcb.109.4.1597. [DOI] [PMC free article] [PubMed] [Google Scholar]

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