Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1997 Apr;65(4):1505–1509. doi: 10.1128/iai.65.4.1505-1509.1997

African strains of Blastomyces dermatitidis that do not express surface adhesin WI-1.

B S Klein 1, B D Aizenstein 1, L H Hogan 1
PMCID: PMC175159  PMID: 9119493

Abstract

African strains of Blastomyces dermatitidis differ from North American strains in their growth, morphology, and clinical disease phenotype. In addition, two serotypes, designated 1 and 2, have been described. We investigated African strains of B. dermatitidis for expression of the surface protein adhesin WI-1 and found that serotype 2 strains do not express it because they lack the coding sequence in their genome. The defect will make the strains useful for gene complementation and for testing the pathogenetic role of the WI-1 adhesin.

Full Text

The Full Text of this article is available as a PDF (223.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradsher R. W. Systemic fungal infections: diagnosis and treatment. I. Blastomycosis. Infect Dis Clin North Am. 1988 Dec;2(4):877–898. [PubMed] [Google Scholar]
  2. Brass C., Volkmann C. M., Philpott D. E., Klein H. P., Halde C. J., Stevens D. A. Spontaneous mutant of Blastomyces dermatitidis attenuated in virulence for mice. Sabouraudia. 1982 Jun;20(2):145–158. [PubMed] [Google Scholar]
  3. Falkow S. Molecular Koch's postulates applied to microbial pathogenicity. Rev Infect Dis. 1988 Jul-Aug;10 (Suppl 2):S274–S276. doi: 10.1093/cid/10.supplement_2.s274. [DOI] [PubMed] [Google Scholar]
  4. Hogan L. H., Josvai S., Klein B. S. Genomic cloning, characterization, and functional analysis of the major surface adhesin WI-1 on Blastomyces dermatitidis yeasts. J Biol Chem. 1995 Dec 22;270(51):30725–30732. doi: 10.1074/jbc.270.51.30725. [DOI] [PubMed] [Google Scholar]
  5. Hogan L. H., Klein B. S. Altered expression of surface alpha-1,3-glucan in genetically related strains of Blastomyces dermatitidis that differ in virulence. Infect Immun. 1994 Aug;62(8):3543–3546. doi: 10.1128/iai.62.8.3543-3546.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kane J. Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C. J Clin Microbiol. 1984 Sep;20(3):594–596. doi: 10.1128/jcm.20.3.594-596.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaufman L., Standard P. G., Weeks R. J., Padhye A. A. Detection of two Blastomyces dermatitidis serotypes by exoantigen analysis. J Clin Microbiol. 1983 Jul;18(1):110–114. doi: 10.1128/jcm.18.1.110-114.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klein B. S., Chaturvedi S., Hogan L. H., Jones J. M., Newman S. L. Altered expression of surface protein WI-1 in genetically related strains of Blastomyces dermatitidis that differ in virulence regulates recognition of yeasts by human macrophages. Infect Immun. 1994 Aug;62(8):3536–3542. doi: 10.1128/iai.62.8.3536-3542.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klein B. S., Hogan L. H., Jones J. M. Immunologic recognition of a 25-amino acid repeat arrayed in tandem on a major antigen of Blastomyces dermatitidis. J Clin Invest. 1993 Jul;92(1):330–337. doi: 10.1172/JCI116571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein B. S., Jones J. M. Isolation, purification, and radiolabeling of a novel 120-kD surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients. J Clin Invest. 1990 Jan;85(1):152–161. doi: 10.1172/JCI114406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klein B. S., Jones J. M. Purification and characterization of the major antigen WI-1 from Blastomyces dermatitidis yeasts and immunological comparison with A antigen. Infect Immun. 1994 Sep;62(9):3890–3900. doi: 10.1128/iai.62.9.3890-3900.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klein B. S., Newman S. L. Role of cell-surface molecules of Blastomyces dermatitidis in host-pathogen interactions. Trends Microbiol. 1996 Jun;4(6):246–251. doi: 10.1016/0966-842X(96)10028-7. [DOI] [PubMed] [Google Scholar]
  13. Klimpel K. R., Goldman W. E. Cell walls from avirulent variants of Histoplasma capsulatum lack alpha-(1,3)-glucan. Infect Immun. 1988 Nov;56(11):2997–3000. doi: 10.1128/iai.56.11.2997-3000.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Newman S. L., Chaturvedi S., Klein B. S. The WI-1 antigen of Blastomyces dermatitidis yeasts mediates binding to human macrophage CD11b/CD18 (CR3) and CD14. J Immunol. 1995 Jan 15;154(2):753–761. [PubMed] [Google Scholar]
  15. San-Blas F., San-Blas G., Cova L. J. A morphological mutant of Paracoccidioides brasiliensis strain IVIC Pb9. Isolation and wall characterization. J Gen Microbiol. 1976 Apr;93(2):209–218. doi: 10.1099/00221287-93-2-209. [DOI] [PubMed] [Google Scholar]
  16. Sarosi G. A., Davies S. F. Blastomycosis. Am Rev Respir Dis. 1979 Oct;120(4):911–938. doi: 10.1164/arrd.1979.120.4.911. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES