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. 1997 Apr;65(4):1519–1521. doi: 10.1128/iai.65.4.1519-1521.1997

Urease-independent chemotactic responses of Helicobacter pylori to urea, urease inhibitors, and sodium bicarbonate.

T Mizote 1, H Yoshiyama 1, T Nakazawa 1
PMCID: PMC175162  PMID: 9119496

Abstract

Helicobacter pylori CPY3401 and an isogenic urease-negative mutant, HPT73, showed chemotactic responses to urea, flurofamide (a potent urease inhibitor), and sodium bicarbonate. Since urea and sodium bicarbonate are secreted through the gastric epithelial surface and hydrolysis of urea by urease on the bacterial surface is essential for colonization, the chemotactic response of H. pylori may be crucial for its colonization and persistence in the stomach.

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Selected References

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  1. Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
  2. Atsumi T., McCarter L., Imae Y. Polar and lateral flagellar motors of marine Vibrio are driven by different ion-motive forces. Nature. 1992 Jan 9;355(6356):182–184. doi: 10.1038/355182a0. [DOI] [PubMed] [Google Scholar]
  3. Blaser M. J. Helicobacter pylori: microbiology of a 'slow' bacterial infection. Trends Microbiol. 1993 Oct;1(7):255–260. doi: 10.1016/0966-842x(93)90047-u. [DOI] [PubMed] [Google Scholar]
  4. Creno R. J., Wenk R. E., Bohlig P. Automated micromeasurement of urea using urease and the Berthelot reaction. Am J Clin Pathol. 1970 Dec;54(6):828–832. doi: 10.1093/ajcp/54.6.828. [DOI] [PubMed] [Google Scholar]
  5. Eaton K. A., Suerbaum S., Josenhans C., Krakowka S. Colonization of gnotobiotic piglets by Helicobacter pylori deficient in two flagellin genes. Infect Immun. 1996 Jul;64(7):2445–2448. doi: 10.1128/iai.64.7.2445-2448.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Falke J. J., Blair D. F., Silhavy T. J., Schmitt R. BLAST 1995: international conference on bacterial locomotion and signal transduction. Mol Microbiol. 1995 Jun;16(6):1037–1050. doi: 10.1111/j.1365-2958.1995.tb02329.x. [DOI] [PubMed] [Google Scholar]
  7. Hazell S. L., Lee A., Brady L., Hennessy W. Campylobacter pyloridis and gastritis: association with intercellular spaces and adaptation to an environment of mucus as important factors in colonization of the gastric epithelium. J Infect Dis. 1986 Apr;153(4):658–663. doi: 10.1093/infdis/153.4.658. [DOI] [PubMed] [Google Scholar]
  8. Hu L. T., Mobley H. L. Purification and N-terminal analysis of urease from Helicobacter pylori. Infect Immun. 1990 Apr;58(4):992–998. doi: 10.1128/iai.58.4.992-998.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Josenhans C., Labigne A., Suerbaum S. Comparative ultrastructural and functional studies of Helicobacter pylori and Helicobacter mustelae flagellin mutants: both flagellin subunits, FlaA and FlaB, are necessary for full motility in Helicobacter species. J Bacteriol. 1995 Jun;177(11):3010–3020. doi: 10.1128/jb.177.11.3010-3020.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kirschner D. E., Blaser M. J. The dynamics of Helicobacter pylori infection of the human stomach. J Theor Biol. 1995 Sep 21;176(2):281–290. doi: 10.1006/jtbi.1995.0198. [DOI] [PubMed] [Google Scholar]
  11. Millner O. E., Jr, Andersen J. A., Appler M. E., Benjamin C. E., Edwards J. G., Humphrey D. T., Shearer E. M. Flurofamide: a potent inhibitor of bacterial urease with potential clinical utility in the treatment of infection induced urinary stones. J Urol. 1982 Feb;127(2):346–350. doi: 10.1016/s0022-5347(17)53779-9. [DOI] [PubMed] [Google Scholar]
  12. Mobley H. L., Cortesia M. J., Rosenthal L. E., Jones B. D. Characterization of urease from Campylobacter pylori. J Clin Microbiol. 1988 May;26(5):831–836. doi: 10.1128/jcm.26.5.831-836.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nagata K., Takagi E., Tsuda M., Nakazawa T., Satoh H., Nakao M., Okamura H., Tamura T. Inhibitory action of lansoprazole and its analogs against Helicobacter pylori: inhibition of growth is not related to inhibition of urease. Antimicrob Agents Chemother. 1995 Feb;39(2):567–570. doi: 10.1128/aac.39.2.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Neithercut W. D., Rowe P. A., el Nujumi A. M., Dahill S., McColl K. E. Effect of Helicobacter pylori infection on intragastric urea and ammonium concentrations in patients with chronic renal failure. J Clin Pathol. 1993 Jun;46(6):544–547. doi: 10.1136/jcp.46.6.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Phadnis S. H., Parlow M. H., Levy M., Ilver D., Caulkins C. M., Connors J. B., Dunn B. E. Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. Infect Immun. 1996 Mar;64(3):905–912. doi: 10.1128/iai.64.3.905-912.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stuart-Tilley A., Sardet C., Pouyssegur J., Schwartz M. A., Brown D., Alper S. L. Immunolocalization of anion exchanger AE2 and cation exchanger NHE-1 in distinct adjacent cells of gastric mucosa. Am J Physiol. 1994 Feb;266(2 Pt 1):C559–C568. doi: 10.1152/ajpcell.1994.266.2.C559. [DOI] [PubMed] [Google Scholar]
  17. Tsuda M., Karita M., Mizote T., Morshed M. G., Okita K., Nakazawa T. Essential role of Helicobacter pylori urease in gastric colonization: definite proof using a urease-negative mutant constructed by gene replacement. Eur J Gastroenterol Hepatol. 1994 Dec;6 (Suppl 1):S49–S52. [PubMed] [Google Scholar]
  18. Tsuda M., Karita M., Morshed M. G., Okita K., Nakazawa T. A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach. Infect Immun. 1994 Aug;62(8):3586–3589. doi: 10.1128/iai.62.8.3586-3589.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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