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. 1997 Apr;65(4):1546–1549. doi: 10.1128/iai.65.4.1546-1549.1997

Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells.

A M Tarkkanen 1, R Virkola 1, S Clegg 1, T K Korhonen 1
PMCID: PMC175168  PMID: 9119502

Abstract

Binding of the two identified type 3 fimbrial variants of Klebsiella pneumoniae to human endothelial EA-hy926 and bladder T24 cells was assessed. The recombinant Escherichia coli strain LE392(pFK12), expressing plasmid-encoded type 3 fimbriae of K. pneumoniae, adhered to both cell lines, and the fimbriae purified from the strain bound to both cell lines in a dose-dependent manner. Adhesiveness to both cell lines of chromosomally encoded type 3 fimbriae from K. pneumoniae IApc35 was lower. No binding was detected with type 1 fimbriae of K. pneumoniae. Both type 3 fimbrial variants exhibited a significantly lower affinity for the cell lines than did S fimbriae of meningitis-associated E. coli.

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Selected References

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