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. 1997 Jan;56(1):41–44. doi: 10.1136/ard.56.1.41

ICAM-1, E-selectin, and TNF α expression in labial salivary glands of patients with rheumatoid vasculitis

R M Flipo 1, T Cardon 1, M C Copin 1, M Vandecandelaere 1, B Duquesnoy 1, A Janin 1
PMCID: PMC1752244  PMID: 9059140

Abstract

OBJECTIVE—To assess the expression of ICAM1, VCAM1, E-selectin, CD44, and TNF α in labial salivary glands of patients with rheumatoid vasculitis.
METHODS—Labial salivary glands from six patients with rheumatoid vasculitis before and after treatment, six aged matched rheumatoid arthritis patients without clinical evidence of rheumatoid vasculitis, 10 patients with primary Sjögren syndrome, 10 patients with rheumatoid arthritis and proven secondary Sjögren syndrome, and six controls were tested with specific antibodies.
RESULTS—ICAM1, E-selectin, and TNF α were significantly expressed in endothelial cells and perivascular cellular infiltrate only in rheumatoid vasculitis before treatment.
CONCLUSIONS—The expression of ICAM1, E-selectin, and TNF α allows an assessment of the activity of the vasculitic process in rheumatoid vasculitis. Labial salivary gland biopsy findings in patients with rheumatoid arthritis and visceral vasculitis without purpura or neuropathy (that is, mesenteric, coronary, or cerebral vasculitis) would be of interest and are examples where labial salivary gland tissue is more accessible than the affected tissue.



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Figure 1  .

Figure 1  

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Labial salivary gland from a patient with rheumatoid vasculitis (patient 4): intense staining with an antibody directed against TNF α in a zone with a dense perivascular infiltrate (arrow = capillary). Indirect immunoperoxidase × 310. 

Figure 2  .

Figure 2  

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Same patient as in fig 1 in clinical remission after treatment. The dense inflammatory infiltrate is still present but there is no staining with the antibody directed against TNF α. Indirect immunoperoxidase × 310. 

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argenbright L. W., Barton R. W. Interactions of leukocyte integrins with intercellular adhesion molecule 1 in the production of inflammatory vascular injury in vivo. The Shwartzman reaction revisited. J Clin Invest. 1992 Jan;89(1):259–272. doi: 10.1172/JCI115570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnett F. C., Edworthy S. M., Bloch D. A., McShane D. J., Fries J. F., Cooper N. S., Healey L. A., Kaplan S. R., Liang M. H., Luthra H. S. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988 Mar;31(3):315–324. doi: 10.1002/art.1780310302. [DOI] [PubMed] [Google Scholar]
  3. Brennan F. M., Maini R. N., Feldmann M. TNF alpha--a pivotal role in rheumatoid arthritis? Br J Rheumatol. 1992 May;31(5):293–298. doi: 10.1093/rheumatology/31.5.293. [DOI] [PubMed] [Google Scholar]
  4. Chisholm D. M., Mason D. K. Labial salivary gland biopsy in Sjögren's disease. J Clin Pathol. 1968 Sep;21(5):656–660. doi: 10.1136/jcp.21.5.656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cronstein B. N., Kimmel S. C., Levin R. I., Martiniuk F., Weissmann G. A mechanism for the antiinflammatory effects of corticosteroids: the glucocorticoid receptor regulates leukocyte adhesion to endothelial cells and expression of endothelial-leukocyte adhesion molecule 1 and intercellular adhesion molecule 1. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):9991–9995. doi: 10.1073/pnas.89.21.9991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cronstein B. N., Weissmann G. The adhesion molecules of inflammation. Arthritis Rheum. 1993 Feb;36(2):147–157. doi: 10.1002/art.1780360204. [DOI] [PubMed] [Google Scholar]
  7. Flipo R. M., Janin A., Hachulla E., Houvenagel E., Foulet A., Cardon T., Desbonnet A., Grardel B., Duquesnoy B., Delcambre B. Labial salivary gland biopsy assessment in rheumatoid vasculitis. Ann Rheum Dis. 1994 Oct;53(10):648–652. doi: 10.1136/ard.53.10.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haynes B. F., Hale L. P., Patton K. L., Martin M. E., McCallum R. M. Measurement of an adhesion molecule as an indicator of inflammatory disease activity. Up-regulation of the receptor for hyaluronate (CD44) in rheumatoid arthritis. Arthritis Rheum. 1991 Nov;34(11):1434–1443. doi: 10.1002/art.1780341115. [DOI] [PubMed] [Google Scholar]
  9. Kraus A., Palacios A., Muñoz L. Muscular involvement in systemic rheumatoid vasculitis. Br J Rheumatol. 1992 May;31(5):355–356. doi: 10.1093/rheumatology/31.5.355. [DOI] [PubMed] [Google Scholar]
  10. Marlor C. W., Webb D. L., Bombara M. P., Greve J. M., Blue M. L. Expression of vascular cell adhesion molecule-1 in fibroblastlike synoviocytes after stimulation with tumor necrosis factor. Am J Pathol. 1992 May;140(5):1055–1060. [PMC free article] [PubMed] [Google Scholar]
  11. McGill P. E., Brougham P. A., Tulloch J. Immune deposits in the skin of patients with rheumatoid arthritis. J Rheumatol. 1984 Aug;11(4):454–456. [PubMed] [Google Scholar]
  12. Mulligan M. S., Wilson G. P., Todd R. F., Smith C. W., Anderson D. C., Varani J., Issekutz T. B., Miyasaka M., Tamatani T., Myasaka M. Role of beta 1, beta 2 integrins and ICAM-1 in lung injury after deposition of IgG and IgA immune complexes. J Immunol. 1993 Mar 15;150(6):2407–2417. [PubMed] [Google Scholar]
  13. Postigo A. A., Garcia-Vicuña R., Diaz-Gonzalez F., Arroyo A. G., De Landázuri M. O., Chi-Rosso G., Lobb R. R., Laffon A., Sánchez-Madrid F. Increased binding of synovial T lymphocytes from rheumatoid arthritis to endothelial-leukocyte adhesion molecule-1 (ELAM-1) and vascular cell adhesion molecule-1 (VCAM-1). J Clin Invest. 1992 May;89(5):1445–1452. doi: 10.1172/JCI115734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Romagnani S. Induction of TH1 and TH2 responses: a key role for the 'natural' immune response? Immunol Today. 1992 Oct;13(10):379–381. doi: 10.1016/0167-5699(92)90083-J. [DOI] [PubMed] [Google Scholar]
  15. Scott D. G., Bacon P. A. Intravenous cyclophosphamide plus methylprednisolone in treatment of systemic rheumatoid vasculitis. Am J Med. 1984 Mar;76(3):377–384. doi: 10.1016/0002-9343(84)90654-5. [DOI] [PubMed] [Google Scholar]
  16. St Clair E. W., Angellilo J. C., Singer K. H. Expression of cell-adhesion molecules in the salivary gland microenvironment of Sjögren's syndrome. Arthritis Rheum. 1992 Jan;35(1):62–66. doi: 10.1002/art.1780350110. [DOI] [PubMed] [Google Scholar]
  17. Tessier P., Audette M., Cattaruzzi P., McColl S. R. Up-regulation by tumor necrosis factor alpha of intercellular adhesion molecule 1 expression and function in synovial fibroblasts and its inhibition by glucocorticoids. Arthritis Rheum. 1993 Nov;36(11):1528–1539. doi: 10.1002/art.1780361107. [DOI] [PubMed] [Google Scholar]
  18. Tribe C. R., Scott D. G., Bacon P. A. Rectal biopsy in the diagnosis of systemic vasculitis. J Clin Pathol. 1981 Aug;34(8):843–850. doi: 10.1136/jcp.34.8.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vitali C., Bombardieri S., Moutsopoulos H. M., Balestrieri G., Bencivelli W., Bernstein R. M., Bjerrum K. B., Braga S., Coll J., de Vita S. Preliminary criteria for the classification of Sjögren's syndrome. Results of a prospective concerted action supported by the European Community. Arthritis Rheum. 1993 Mar;36(3):340–347. doi: 10.1002/art.1780360309. [DOI] [PubMed] [Google Scholar]
  20. Vollertsen R. S., Conn D. L., Ballard D. J., Ilstrup D. M., Kazmar R. E., Silverfield J. C. Rheumatoid vasculitis: survival and associated risk factors. Medicine (Baltimore) 1986 Nov;65(6):365–375. [PubMed] [Google Scholar]

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