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. 2000 Oct;59(10):775–780. doi: 10.1136/ard.59.10.775

Monocyte chemoattractant protein 1 (MCP-1) in temporal arteritis and polymyalgia rheumatica

T Ellingsen 1, P Elling 1, A Olson 1, H Elling 1, U Baandrup 1, K Matsushima 1, B Deleuran 1, K Stengaard-Pederse 1
PMCID: PMC1753013  PMID: 11005777

Abstract

OBJECTIVE—To examine the localisation of monocyte chemoattractant protein 1 (MCP-1) in the inflamed vessel wall in temporal arteritis (TA) and to measure MCP-1 in plasma both in patients with TA and patients with polymyalgia rheumatica (PMR).
METHODS—By immunohistochemical techniques MCP-1 was localised to the vessel wall in patients with TA. In TA, PMR, and healthy controls MCP-1 was quantified by enzyme linked immunosorbent assay (ELISA) in plasma.
RESULTS—MCP-1 was localised to the majority of mononuclear cells, some smooth muscle cells, and giant cells in the arterial biopsy specimens from 12 patients with histologically verified TA. In all sections, including the vasa vasorum, the endothelium stained positive. In the intima 73% (range 57-91%), in the media 49% (range 32-67%), and in the adventitia 74% (range of 62-91%) of all cells stained positive. In plasma MCP-1 was significantly raised in untreated TA (n=33) and untreated PMR (n=27) compared with healthy controls (n=12). Untreated TA plasma levels of MCP-1 (mean 391 pg/ml (range 82-778 pg/ml)) were similar to untreated PMR plasma levels (mean 402 pg/ml (range 29-1153 pg/ml)), and no significant difference was found between the two groups of patients. In both patients with TA and patients with PMR no correlation was found between the plasma level of MCP-1 and the erythrocyte sedimentation rate, haemoglobin concentration, and CD4/CD8 ratio.
CONCLUSIONS—These results show that MCP-1 plays a part in the disease processes of TA and PMR.



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Figure 1  .

Figure 1  

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Overview of a section of the temporal artery (magnification ×16) showing differentiated mononuclear infiltration in relation to the lamina elastica externa, marked with arrows. Positive staining appears red, streptavidine-alkaline phosphatase.

Figure 2  .

Figure 2  

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(a) Section of the temporal artery (magnification ×80) showing the distribution of MCP-1 positive cells located in the adventitia, media, and intima. Staining of the vasa vasorum in the adventitia is shown (point A). Mononuclear cell infiltration adjacent to the lamina elastica externa (point B). (b) Control staining of section from the same biopsy (substitution of the primary anti MCP-1 antibody with IgG of the same species). Positive staining appears red, streptavidine-alkaline phosphatase.

Figure 3  .

Figure 3  

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Staining of smooth muscle cells in the media, marked by arrows. Magnification ×200. Positive staining appears red (streptavidine-alkaline phosphatase).

Figure 4  .

Figure 4  

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Bio-Ra-anti-MCP-1 is specific on Western blotting (sodium dodecyl sulphate-polyacrylamide gel electrophoresis using 12% gels) analysing IL1α stimulated MNC (lane 3), giving only one band equalling hrMCP-1 (lane 2). Lane 1 = molecular weight marker.

Figure 5  .

Figure 5  

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Raised levels of plasma MCP-1 were found in untreated patients with temporal arteritis and polymyalgia rheumatica compared with healthy controls.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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