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. 1997 Jun;65(6):2434–2436. doi: 10.1128/iai.65.6.2434-2436.1997

Chlamydia trachomatis growth stimulates interleukin 8 production by human monocytic U-937 cells.

A Bianchi 1, C Dosquet 1, S Henry 1, M C Couderc 1, F Ferchal 1, C Scieux 1
PMCID: PMC175337  PMID: 9169785

Abstract

Growth of Chlamydia trachomatis serotypes L2 and L3 in a human monocytic cell line, U-937, increased the rate of interleukin 8 (IL-8) release 100-fold. Heat-killed chlamydiae induced a 10-fold-lower level of production of IL-8. IL-8 may play an important role in the inflammatory reaction to chlamydial infection.

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Selected References

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  1. Arno J. N., Ricker V. A., Batteiger B. E., Katz B. P., Caine V. A., Jones R. B. Interferon-gamma in endocervical secretions of women infected with Chlamydia trachomatis. J Infect Dis. 1990 Dec;162(6):1385–1389. doi: 10.1093/infdis/162.6.1385. [DOI] [PubMed] [Google Scholar]
  2. Cain T. K., Rank R. G. Local Th1-like responses are induced by intravaginal infection of mice with the mouse pneumonitis biovar of Chlamydia trachomatis. Infect Immun. 1995 May;63(5):1784–1789. doi: 10.1128/iai.63.5.1784-1789.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Darville T., Laffoon K. K., Kishen L. R., Rank R. G. Tumor necrosis factor alpha activity in genital tract secretions of guinea pigs infected with chlamydiae. Infect Immun. 1995 Dec;63(12):4675–4681. doi: 10.1128/iai.63.12.4675-4681.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Demczuk S., Baumberger C., Mach B., Dayer J. M. Differential effects of in vitro mycoplasma infection on interleukin-1 alpha and beta mRNA expression in U937 and A431 cells. J Biol Chem. 1988 Sep 15;263(26):13039–13045. [PubMed] [Google Scholar]
  5. Eckmann L., Kagnoff M. F., Fierer J. Epithelial cells secrete the chemokine interleukin-8 in response to bacterial entry. Infect Immun. 1993 Nov;61(11):4569–4574. doi: 10.1128/iai.61.11.4569-4574.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herbelin A., Ruuth E., Delorme D., Michel-Herbelin C., Praz F. Mycoplasma arginini TUH-14 membrane lipoproteins induce production of interleukin-1, interleukin-6, and tumor necrosis factor alpha by human monocytes. Infect Immun. 1994 Oct;62(10):4690–4694. doi: 10.1128/iai.62.10.4690-4694.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ingalls R. R., Rice P. A., Qureshi N., Takayama K., Lin J. S., Golenbock D. T. The inflammatory cytokine response to Chlamydia trachomatis infection is endotoxin mediated. Infect Immun. 1995 Aug;63(8):3125–3130. doi: 10.1128/iai.63.8.3125-3130.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Liles W. C., Van Voorhis W. C. Review: nomenclature and biologic significance of cytokines involved in inflammation and the host immune response. J Infect Dis. 1995 Dec;172(6):1573–1580. doi: 10.1093/infdis/172.6.1573. [DOI] [PubMed] [Google Scholar]
  9. Magee D. M., Smith J. G., Bleicker C. A., Carter C. J., Bonewald L. F., Schachter J., Williams D. M. Chlamydia trachomatis pneumonia induces in vivo production of interleukin-1 and -6. Infect Immun. 1992 Mar;60(3):1217–1220. doi: 10.1128/iai.60.3.1217-1220.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Magee D. M., Williams D. M., Wing E. J., Bleicker C. A., Schachter J. Production of colony-stimulating factors during pneumonia caused by Chlamydia trachomatis. Infect Immun. 1991 Jul;59(7):2370–2375. doi: 10.1128/iai.59.7.2370-2375.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McElvaney N. G., Nakamura H., Birrer P., Hébert C. A., Wong W. L., Alphonso M., Baker J. B., Catalano M. A., Crystal R. G. Modulation of airway inflammation in cystic fibrosis. In vivo suppression of interleukin-8 levels on the respiratory epithelial surface by aerosolization of recombinant secretory leukoprotease inhibitor. J Clin Invest. 1992 Oct;90(4):1296–1301. doi: 10.1172/JCI115994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oishi K., Sonoda F., Kobayashi S., Iwagaki A., Nagatake T., Matsushima K., Matsumoto K. Role of interleukin-8 (IL-8) and an inhibitory effect of erythromycin on IL-8 release in the airways of patients with chronic airway diseases. Infect Immun. 1994 Oct;62(10):4145–4152. doi: 10.1128/iai.62.10.4145-4152.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ozaki T., Hayashi H., Tani K., Ogushi F., Yasuoka S., Ogura T. Neutrophil chemotactic factors in the respiratory tract of patients with chronic airway diseases or idiopathic pulmonary fibrosis. Am Rev Respir Dis. 1992 Jan;145(1):85–91. doi: 10.1164/ajrccm/145.1.85. [DOI] [PubMed] [Google Scholar]
  14. Rawadi G., Roman-Roman S. Mycoplasma membrane lipoproteins induced proinflammatory cytokines by a mechanism distinct from that of lipopolysaccharide. Infect Immun. 1996 Feb;64(2):637–643. doi: 10.1128/iai.64.2.637-643.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Richman-Eisenstat J. B., Jorens P. G., Hébert C. A., Ueki I., Nadel J. A. Interleukin-8: an important chemoattractant in sputum of patients with chronic inflammatory airway diseases. Am J Physiol. 1993 Apr;264(4 Pt 1):L413–L418. doi: 10.1152/ajplung.1993.264.4.L413. [DOI] [PubMed] [Google Scholar]
  16. Rothermel C. D., Schachter J., Lavrich P., Lipsitz E. C., Francus T. Chlamydia trachomatis-induced production of interleukin-1 by human monocytes. Infect Immun. 1989 Sep;57(9):2705–2711. doi: 10.1128/iai.57.9.2705-2711.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sundström C., Nilsson K. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int J Cancer. 1976 May 15;17(5):565–577. doi: 10.1002/ijc.2910170504. [DOI] [PubMed] [Google Scholar]
  18. Teyssou R., Poutiers F., Saillard C., Grau O., Laigret F., Bové J. M., Bébéar C. Detection of mollicute contamination in cell cultures by 16S rDNA amplification. Mol Cell Probes. 1993 Jun;7(3):209–216. doi: 10.1006/mcpr.1993.1030. [DOI] [PubMed] [Google Scholar]
  19. Toth M., Jeremias J., Ledger W. J., Witkin S. S. In vivo tumor necrosis factor production in women with salpingitis. Surg Gynecol Obstet. 1992 May;174(5):359–362. [PubMed] [Google Scholar]
  20. Williams D. M., Bonewald L. F., Roodman G. D., Byrne G. I., Magee D. M., Schachter J. Tumor necrosis factor alpha is a cytotoxin induced by murine Chlamydia trachomatis infection. Infect Immun. 1989 May;57(5):1351–1355. doi: 10.1128/iai.57.5.1351-1355.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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