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Figure 1 .
RNA immunoprecipitation. Urea (7 M/10% polyacrylamide gel electrophoresis of phenol-extracted immunoprecipitates from HeLa cell extracts were stained with silver. Total nucleic acids, with 7-2RNA, 8-2RNA, and the U snRNA regions are indicated. Serum samples used for immunoprecipitation included: lane 1, total RNA; lane 2, healthy control serum; lanes 3-4, patients with LScl and with anti-U3 snRNP antibodies; lane 5, patient with SSc and anti-Th/To ribonucleoprotein antibodies; lane 6, patient with SSc and anti-U3 snRNP antibodies; lane 7, patient with systemic lupus erythematosus and anti-Sm antibodies.