Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1997 Jun;65(6):2468–2471. doi: 10.1128/iai.65.6.2468-2471.1997

Adherence of Mycoplasma gallisepticum involves variable surface membrane proteins.

A Athamna 1, R Rosengarten 1, S Levisohn 1, I Kahane 1, D Yogev 1
PMCID: PMC175345  PMID: 9169793

Abstract

Adherence of Mycoplasma gallisepticum to erythrocytes was examined by colony immunoblotting, detergent phase fractionation, trypsin treatment, comparison of protein profiles, and comparison of erythrocyte-bound mycoplasma protein fractions of hemadsorption-positive and -negative mutants. The binding of M. gallisepticum to chicken or human erythrocytes was found to be mediated via surface-exposed membrane proteins undergoing high-frequency phase variation.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham J. M., Freitag C. S., Clements J. R., Eisenstein B. I. An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5724–5727. doi: 10.1073/pnas.82.17.5724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banai M., Kahane I., Razin S., Bredt W. Adherence of Mycoplasma gallisepticum to human erythrocytes. Infect Immun. 1978 Aug;21(2):365–372. doi: 10.1128/iai.21.2.365-372.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baseman J. B., Cole R. M., Krause D. C., Leith D. K. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol. 1982 Sep;151(3):1514–1522. doi: 10.1128/jb.151.3.1514-1522.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Behrens A., Heller M., Kirchhoff H., Yogev D., Rosengarten R. A family of phase- and size-variant membrane surface lipoprotein antigens (Vsps) of Mycoplasma bovis. Infect Immun. 1994 Nov;62(11):5075–5084. doi: 10.1128/iai.62.11.5075-5084.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collier A. M., Clyde W. A., Jr Appearance of Mycoplasma pneumoniae in lungs of experimentally infected hamsters and sputum from patients with natural disease. Am Rev Respir Dis. 1974 Dec;110(6):765–773. doi: 10.1164/arrd.1974.110.6P1.765. [DOI] [PubMed] [Google Scholar]
  6. Glew M. D., Markham P. F., Browning G. F., Walker I. D. Expression studies on four members of the pMGA multigene family in Mycoplasma gallisepticum S6. Microbiology. 1995 Nov;141(Pt 11):3005–3014. doi: 10.1099/13500872-141-11-3005. [DOI] [PubMed] [Google Scholar]
  7. Hansen E. J., Wilson R. M., Baseman J. B. Isolation of mutants of Mycoplasma pneumoniae defective in hemadsorption. Infect Immun. 1979 Mar;23(3):903–906. doi: 10.1128/iai.23.3.903-906.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hansen E. J., Wilson R. M., Clyde W. A., Jr, Baseman J. B. Characterization of hemadsorption-negative mutants of Mycoplasma pneumoniae. Infect Immun. 1981 Apr;32(1):127–136. doi: 10.1128/iai.32.1.127-136.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kahane I. Adhesion of mycoplasmas. Methods Enzymol. 1995;253:367–373. doi: 10.1016/s0076-6879(95)53031-2. [DOI] [PubMed] [Google Scholar]
  10. Kahane I., Horowitz S. Adherence of mycoplasma to cell surfaces. Subcell Biochem. 1993;20:225–241. doi: 10.1007/978-1-4615-2924-8_8. [DOI] [PubMed] [Google Scholar]
  11. Krause D. C., Baseman J. B. Mycoplasma pneumoniae proteins that selectively bind to host cells. Infect Immun. 1982 Jul;37(1):382–386. doi: 10.1128/iai.37.1.382-386.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Krause D. C., Leith D. K., Baseman J. B. Reacquisition of specific proteins confers virulence in Mycoplasma pneumoniae. Infect Immun. 1983 Feb;39(2):830–836. doi: 10.1128/iai.39.2.830-836.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Krause D. C., Leith D. K., Wilson R. M., Baseman J. B. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect Immun. 1982 Mar;35(3):809–817. doi: 10.1128/iai.35.3.809-817.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lipman R. P., Clyde W. A., Jr The interrelationship of virulence, cytadsorption, and peroxide formation in Mycoplasma pneumoniae. Proc Soc Exp Biol Med. 1969 Sep;131(4):1163–1167. doi: 10.3181/00379727-131-34061. [DOI] [PubMed] [Google Scholar]
  16. Manchee R. J., Taylor-Robinson D. Haemadsorption and haemagglutination by mycoplasmas. J Gen Microbiol. 1968 Mar;50(3):465–478. doi: 10.1099/00221287-50-3-465. [DOI] [PubMed] [Google Scholar]
  17. Manchee R. J., Taylor-Robinson D. Utilization of neuraminic acid receptors by mycoplasmas. J Bacteriol. 1969 Jun;98(3):914–919. doi: 10.1128/jb.98.3.914-919.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Markham P. F., Glew M. D., Brandon M. R., Walker I. D., Whithear K. G. Characterization of a major hemagglutinin protein from Mycoplasma gallisepticum. Infect Immun. 1992 Sep;60(9):3885–3891. doi: 10.1128/iai.60.9.3885-3891.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Murphy G. L., Cannon J. G. Genetics of surface protein variation in Neisseria gonorrhoeae. Bioessays. 1988 Jul;9(1):7–11. doi: 10.1002/bies.950090103. [DOI] [PubMed] [Google Scholar]
  20. Razin S., Jacobs E. Mycoplasma adhesion. J Gen Microbiol. 1992 Mar;138(3):407–422. doi: 10.1099/00221287-138-3-407. [DOI] [PubMed] [Google Scholar]
  21. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Razin S. Peculiar properties of mycoplasmas: the smallest self-replicating prokaryotes. FEMS Microbiol Lett. 1992 Dec 15;100(1-3):423–431. doi: 10.1111/j.1574-6968.1992.tb14072.x. [DOI] [PubMed] [Google Scholar]
  23. Robertson J. A., Sherburne R. Hemadsorption by colonies of Ureaplasma urealyticum. Infect Immun. 1991 Jun;59(6):2203–2206. doi: 10.1128/iai.59.6.2203-2206.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rosengarten R., Wise K. S. Phenotypic switching in mycoplasmas: phase variation of diverse surface lipoproteins. Science. 1990 Jan 19;247(4940):315–318. doi: 10.1126/science.1688663. [DOI] [PubMed] [Google Scholar]
  25. Rosengarten R., Yogev D. Variant colony surface antigenic phenotypes within mycoplasma strain populations: implications for species identification and strain standardization. J Clin Microbiol. 1996 Jan;34(1):149–158. doi: 10.1128/jcm.34.1.149-158.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Weiss A. A., Falkow S. Genetic analysis of phase change in Bordetella pertussis. Infect Immun. 1984 Jan;43(1):263–269. doi: 10.1128/iai.43.1.263-269.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES