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Infection and Immunity logoLink to Infection and Immunity
. 1997 Jun;65(6):2475–2479. doi: 10.1128/iai.65.6.2475-2479.1997

The lipopolysaccharide O side chain of Vibrio vulnificus serogroup E is a virulence determinant for eels.

C Amaro 1, B Fouz 1, E G Biosca 1, E Marco-Noales 1, R Collado 1
PMCID: PMC175347  PMID: 9169795

Abstract

Vibrio vulnificus is a gram-negative bacterium capable of producing septicemic infections in eels and immunocompromised humans. Two biotypes are classically recognized, with the virulence for eels being specific to strains belonging to biotype 2, which constitutes a homogeneous lipopolysaccharide (LPS)-based O serogroup (which we have designated serogroup E). In the present study we demonstrated that the O side chain of this LPS determines the selective virulence of biotype 2 for eels: (i) biotype 1 strains (which do not belong to serogroup E) are destroyed by the bactericidal action of nonimmune eel serum (NIS) through activation of the alternative pathway of complement, (ii) biotype 2 strains (of serogroup E) are resistant to NIS, and (iii) rough mutants of biotype 2 lacking the O polysaccharide side chain are sensitive to NIS and avirulent for eels.

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Selected References

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  1. Amaro C., Biosca E. G., Fouz B., Alcaide E., Esteve C. Evidence that water transmits Vibrio vulnificus biotype 2 infections to eels. Appl Environ Microbiol. 1995 Mar;61(3):1133–1137. doi: 10.1128/aem.61.3.1133-1137.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amaro C., Biosca E. G., Fouz B., Garay E. Electrophoretic analysis of heterogeneous lipopolysaccharides from various strains of Vibrio vulnificus biotypes 1 and 2 by silver staining and immunoblotting. Curr Microbiol. 1992 Aug;25(2):99–104. doi: 10.1007/BF01570967. [DOI] [PubMed] [Google Scholar]
  3. Amaro C., Biosca E. G., Fouz B., Toranzo A. E., Garay E. Role of iron, capsule, and toxins in the pathogenicity of Vibrio vulnificus biotype 2 for mice. Infect Immun. 1994 Feb;62(2):759–763. doi: 10.1128/iai.62.2.759-763.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Amaro C., Biosca E. G. Vibrio vulnificus biotype 2, pathogenic for eels, is also an opportunistic pathogen for humans. Appl Environ Microbiol. 1996 Apr;62(4):1454–1457. doi: 10.1128/aem.62.4.1454-1457.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Binns M. M., Vaughan S., Timmis K. N. 'O'-antigens are essential virulence factors of Shigella sonnei and Shigella dysenteriae 1. Zentralbl Bakteriol Mikrobiol Hyg B. 1985 Jun;181(1-2):197–205. [PubMed] [Google Scholar]
  6. Biosca E. G., Amaro C., Larsen J. L., Pedersen K. Phenotypic and genotypic characterization of Vibrio vulnificus: proposal for the substitution of the subspecific taxon biotype for serovar. Appl Environ Microbiol. 1997 Apr;63(4):1460–1466. doi: 10.1128/aem.63.4.1460-1466.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Biosca E. G., Amaro C. Toxic and enzymatic activities of Vibrio vulnificus biotype 2 with respect to host specificity. Appl Environ Microbiol. 1996 Jul;62(7):2331–2337. doi: 10.1128/aem.62.7.2331-2337.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Biosca E. G., Fouz B., Alcaide E., Amaro C. Siderophore-mediated iron acquisition mechanisms in Vibrio vulnificus biotype 2. Appl Environ Microbiol. 1996 Mar;62(3):928–935. doi: 10.1128/aem.62.3.928-935.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Biosca E. G., Garay E., Toranzo A. E., Amaro C. Comparison of outer membrane protein profiles of Vibrio vulnificus biotypes 1 and 2. FEMS Microbiol Lett. 1993 Mar 1;107(2-3):217–222. doi: 10.1111/j.1574-6968.1993.tb06033.x. [DOI] [PubMed] [Google Scholar]
  10. Biosca E. G., Llorens H., Garay E., Amaro C. Presence of a capsule in Vibrio vulnificus biotype 2 and its relationship to virulence for eels. Infect Immun. 1993 May;61(5):1611–1618. doi: 10.1128/iai.61.5.1611-1618.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Burreson E. M., Frizzell L. J. The seasonal antibody response in juvenile summer flounder (Paralichthys dentatus) to the hemoflagellate Trypanoplasma bullocki. Vet Immunol Immunopathol. 1986 Jun;12(1-4):395–402. doi: 10.1016/0165-2427(86)90146-7. [DOI] [PubMed] [Google Scholar]
  12. Cipriano R. C., Pyle J. B., Starliper C. E., Pyle S. W. Detection of Vibrio anguillarum antigen by the dot blot assay. J Wildl Dis. 1985 Jul;21(3):211–218. doi: 10.7589/0090-3558-21.3.211. [DOI] [PubMed] [Google Scholar]
  13. Eidinger D., Bello E., Mates A. The heterocytotoxicity of human serum. I. Activation of the alternative complement pathway by heterologous target cells. Cell Immunol. 1977 Mar 1;29(1):174–186. doi: 10.1016/0008-8749(77)90286-6. [DOI] [PubMed] [Google Scholar]
  14. Engels W., Endert J., Kamps M. A., van Boven C. P. Role of lipopolysaccharide in opsonization and phagocytosis of Pseudomonas aeruginosa. Infect Immun. 1985 Jul;49(1):182–189. doi: 10.1128/iai.49.1.182-189.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Fine D. P., Marney S. R., Jr, Colley D. G., Sergent J. S., Des Prez R. M. C3 shunt activation in human serum chelated with EGTA. J Immunol. 1972 Oct;109(4):807–809. [PubMed] [Google Scholar]
  16. Fouz B., Mazoy R., Lemos M. L., del Olmo M. J., Amaro C. Utilization of hemin and hemoglobin by Vibrio vulnificus biotype 2. Appl Environ Microbiol. 1996 Aug;62(8):2806–2810. doi: 10.1128/aem.62.8.2806-2810.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Helms S. D., Oliver J. D., Travis J. C. Role of heme compounds and haptoglobin in Vibrio vulnificus pathogenicity. Infect Immun. 1984 Aug;45(2):345–349. doi: 10.1128/iai.45.2.345-349.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hoben H. J., Somasegaran P. Comparison of the Pour, Spread, and Drop Plate Methods for Enumeration of Rhizobium spp. in Inoculants Made from Presterilized Peat. Appl Environ Microbiol. 1982 Nov;44(5):1246–1247. doi: 10.1128/aem.44.5.1246-1247.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kreger A., DeChatelet L., Shirley P. Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. J Infect Dis. 1981 Sep;144(3):244–248. doi: 10.1093/infdis/144.3.244. [DOI] [PubMed] [Google Scholar]
  20. Kreger A., Lockwood D. Detection of extracellular toxin(s) produced by Vibrio vulnificus. Infect Immun. 1981 Aug;33(2):583–590. doi: 10.1128/iai.33.2.583-590.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Martin S. J., Siebeling R. J. Identification of Vibrio vulnificus O serovars with antilipopolysaccharide monoclonal antibody. J Clin Microbiol. 1991 Aug;29(8):1684–1688. doi: 10.1128/jcm.29.8.1684-1688.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Matsuyama H., Tanaka K., Nakao M., Yano T. Characterization of the alternative complement pathway of carp. Dev Comp Immunol. 1988 Spring;12(2):403–408. doi: 10.1016/0145-305x(88)90015-8. [DOI] [PubMed] [Google Scholar]
  23. Merino S., Albertí S., Tomás J. M. Aeromonas salmonicida resistance to complement-mediated killing. Infect Immun. 1994 Dec;62(12):5483–5490. doi: 10.1128/iai.62.12.5483-5490.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mäkelä P. H., Hovi M., Saxén H., Valtonen M., Valtonen V. Salmonella, complement and mouse macrophages. Immunol Lett. 1988 Nov;19(3):217–222. doi: 10.1016/0165-2478(88)90145-9. [DOI] [PubMed] [Google Scholar]
  25. Oliver J. D., Warner R. A., Cleland D. R. Distribution and ecology of Vibrio vulnificus and other lactose-fermenting marine vibrios in coastal waters of the southeastern United States. Appl Environ Microbiol. 1982 Dec;44(6):1404–1414. doi: 10.1128/aem.44.6.1404-1414.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Simpson L. M., Oliver J. D. Siderophore production by Vibrio vulnificus. Infect Immun. 1983 Aug;41(2):644–649. doi: 10.1128/iai.41.2.644-649.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sunyer J. O., Tort L. Natural hemolytic and bactericidal activities of sea bream Sparus aurata serum are effected by the alternative complement pathway. Vet Immunol Immunopathol. 1995 Apr;45(3-4):333–345. doi: 10.1016/0165-2427(94)05430-z. [DOI] [PubMed] [Google Scholar]
  28. Tison D. L., Nishibuchi M., Greenwood J. D., Seidler R. J. Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl Environ Microbiol. 1982 Sep;44(3):640–646. doi: 10.1128/aem.44.3.640-646.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yoshida S., Ogawa M., Mizuguchi Y. Relation of capsular materials and colony opacity to virulence of Vibrio vulnificus. Infect Immun. 1985 Feb;47(2):446–451. doi: 10.1128/iai.47.2.446-451.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. al-Hendy A., Toivanen P., Skurnik M. Lipopolysaccharide O side chain of Yersinia enterocolitica O:3 is an essential virulence factor in an orally infected murine model. Infect Immun. 1992 Mar;60(3):870–875. doi: 10.1128/iai.60.3.870-875.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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