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. 1997 Jun;65(6):2480–2482. doi: 10.1128/iai.65.6.2480-2482.1997

Binding of Helicobacter pylori to sialic acid-containing glycolipids of various origins separated on thin-layer chromatograms.

H Miller-Podraza 1, M A Milh 1, S Teneberg 1, K A Karlsson 1
PMCID: PMC175348  PMID: 9169796

Abstract

Two standard strains of Helicobacter pylori, grown on solid or in liquid medium, were studied for their binding to sialic acid-containing glycosphingolipids on thin-layer plates. NCTC 11637, but not strain 11638, bound to mixtures of gangliosides of various human and animal origins with similar binding patterns and also to polyglycosylceramides of human erythrocytes, leukocytes, and placenta. There was an apparent specificity for NeuAc alpha3Gal of the neolacto series of gangliosides, since NeuAc alpha6Gal or ganglio-series gangliosides did not bind.

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Selected References

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  1. Evans D. G., Evans D. J., Jr Adhesion properties of Helicobacter pylori. Methods Enzymol. 1995;253:336–360. doi: 10.1016/s0076-6879(95)53029-0. [DOI] [PubMed] [Google Scholar]
  2. Evans D. G., Evans D. J., Jr, Moulds J. J., Graham D. Y. N-acetylneuraminyllactose-binding fibrillar hemagglutinin of Campylobacter pylori: a putative colonization factor antigen. Infect Immun. 1988 Nov;56(11):2896–2906. doi: 10.1128/iai.56.11.2896-2906.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. Hirmo S., Kelm S., Schauer R., Nilsson B., Wadström T. Adhesion of Helicobacter pylori strains to alpha-2,3-linked sialic acids. Glycoconj J. 1996 Dec;13(6):1005–1011. doi: 10.1007/BF01053196. [DOI] [PubMed] [Google Scholar]
  5. Huesca M., Borgia S., Hoffman P., Lingwood C. A. Acidic pH changes receptor binding specificity of Helicobacter pylori: a binary adhesion model in which surface heat shock (stress) proteins mediate sulfatide recognition in gastric colonization. Infect Immun. 1996 Jul;64(7):2643–2648. doi: 10.1128/iai.64.7.2643-2648.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kamisago S., Iwamori M., Tai T., Mitamura K., Yazaki Y., Sugano K. Role of sulfatides in adhesion of Helicobacter pylori to gastric cancer cells. Infect Immun. 1996 Feb;64(2):624–628. doi: 10.1128/iai.64.2.624-628.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karlsson K. A. Preparation of total nonacid glycolipids for overlay analysis of receptors for bacteria and viruses and for other studies. Methods Enzymol. 1987;138:212–220. doi: 10.1016/0076-6879(87)38018-8. [DOI] [PubMed] [Google Scholar]
  8. Lelwala-Guruge J., Ljungh A., Wadström T. Haemagglutination patterns of Helicobacter pylori. Frequency of sialic acid-specific and non-sialic acid-specific haemagglutinins. APMIS. 1992 Oct;100(10):908–913. [PubMed] [Google Scholar]
  9. Miller-Podraza H., Andersson C., Karlsson K. A. New method for the isolation of polyglycosylceramides from human erythrocyte membranes. Biochim Biophys Acta. 1993 Jul 1;1168(3):330–339. doi: 10.1016/0005-2760(93)90190-k. [DOI] [PubMed] [Google Scholar]
  10. Miller-Podraza H., Milh M. A., Bergström J., Karlsson K. A. Recognition of glycoconjugates by Helicobacter pylori: an apparently high-affinity binding of human polyglycosylceramides, a second sialic acid-based specificity. Glycoconj J. 1996 Jun;13(3):453–460. doi: 10.1007/BF00731478. [DOI] [PubMed] [Google Scholar]
  11. Saitoh T., Natomi H., Zhao W. L., Okuzumi K., Sugano K., Iwamori M., Nagai Y. Identification of glycolipid receptors for Helicobacter pylori by TLC-immunostaining. FEBS Lett. 1991 May 6;282(2):385–387. doi: 10.1016/0014-5793(91)80519-9. [DOI] [PubMed] [Google Scholar]
  12. Stroud M. R., Handa K., Ito K., Salyan M. E., Fang H., Levery S. B., Hakamori S., Reinhold B. B., Reinhold V. N. Myeloglycan, a series of E-selectin-binding polylactosaminolipids found in normal human leukocytes and myelocytic leukemia HL60 cells. Biochem Biophys Res Commun. 1995 Apr 26;209(3):777–787. doi: 10.1006/bbrc.1995.1568. [DOI] [PubMed] [Google Scholar]
  13. Stroud M. R., Handa K., Salyan M. E., Ito K., Levery S. B., Hakomori S., Reinhold B. B., Reinhold V. N. Monosialogangliosides of human myelogenous leukemia HL60 cells and normal human leukocytes. 2. Characterization of E-selectin binding fractions, and structural requirements for physiological binding to E-selectin. Biochemistry. 1996 Jan 23;35(3):770–778. doi: 10.1021/bi952461g. [DOI] [PubMed] [Google Scholar]

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