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Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 2001 Mar;60(3):242–247. doi: 10.1136/ard.60.3.242

Prolactin enhances the in vitro production of IgG in peripheral blood mononuclear cells from patients with systemic lupus erythematosus but not from healthy controls

A Jacobi 1, W Rohde 1, H Volk 1, T Dorner 1, G Burmester 1, F Hiepe 1
PMCID: PMC1753559  PMID: 11171686

Abstract

OBJECTIVES—Recent evidence suggests that prolactin (PRL) plays a part in the pathogenesis of systemic lupus erythematosus (SLE). Because B cell hyperreactivity and autoantibodies are characteristic hallmarks of SLE, this study aimed at assessing the impact of this pituitary hormone on IgG production by stimulating peripheral blood mononuclear cells (PBMC) with PRL.
METHODS—PBMC from 11 patients with SLE assessed by the ECLAM score and eight healthy controls were incubated with PRL and cultured for seven days. IgG production was measured by enzyme linked immunosorbent assay (ELISA).
RESULTS—Spontaneous IgG production of SLE PBMC was significantly enhanced compared with that found in healthy controls. After PRL stimulation, the IgG concentrations of supernatants from SLE PBMC were significantly higher than those of unstimulated PBMC (median 394 ng/ml). Of note, the physiological concentration of PRL (20 ng/ml) induced IgG production more effectively (median 1139 ng/ml) than PRL at 100 ng/ml (median 1029 ng/ml). In contrast, preincubation with PRL did not stimulate IgG production in normal PBMC. A significant correlation between PRL induced IgG production and the disease activity (ECLAM) of the patients with SLE was seen. Moreover, the maximum amount of PRL induced IgG depended on the serum PRL concentrations of the patients with SLE.
CONCLUSIONS—The results suggest that PBMC from patients with SLE have an extraordinarily high susceptibility to PRL, showing the most striking effect at a concentration usually found in vivo. This indicates a potential role for mild hyperprolactinaemia in the pathogenesis of SLE, influencing both IgG production and disease activity.



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Figure 1  .

Figure 1  

Concentration of total IgG in the supernatants of peripheral blood mononuclear cells (PBMC) obtained from 11 patients with systemic lupus erythematosus (SLE) after incubation with prolactin (PRL) at concentrations of 20 and 100 ng/ml, respectively, and without PRL (0) for seven days. Significant differences were seen between the IgG production by PBMC from patients with SLE after and without stimulation with PRL (20 and 100 ng/ml, respectively) (Wilcoxon test and Bonferroni correction, p=0.043).

Figure 2  .

Figure 2  

Correlation of the IgG concentration of the supernatants of peripheral blood mononuclear cells from patients with systemic lupus erythematosus (SLE) after incubation with prolactin (PRL) at two concentrations (CPRL = 20 ng/ml and 100 ng/ml) and the ECLAM score of the patients with SLE analysed.

Selected References

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