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. 2003 Mar;62(3):236–241. doi: 10.1136/ard.62.3.236

Myeloid related protein 8 and 14 secretion reflects phagocyte activation and correlates with disease activity in juvenile idiopathic arthritis treated with autologous stem cell transplantation

N Wulffraat 1, P Haas 1, M Frosch 1, I M de Kleer 1, T Vogl 1, D Brinkman 1, P Quartier 1, J Roth 1, W Kuis 1
PMCID: PMC1754451  PMID: 12594109

Abstract

Objectives: To determine whether myeloid related proteins (MRP8/MRP14), a complex of two S100 proteins related to neutrophil and monocyte activation, might be used as a marker for disease activity, and as an early indicator of relapse in juvenile idiopathic arthritis.

Patients and methods: A group of 12 patients who underwent an autologous haematopoietic stem cell transplantation (ASCT) for refractory juvenile idiopathic arthritis (JIA) were studied. MRP8/MRP14 serum concentrations were determined by a sandwich enzyme linked immunosorbent assay (ELISA) as described. Improvement from baseline was described by a definition of improvement employing a core set of criteria as detailed previously by Giannini.

Results: After ASCT, MRP8/MRP14 serum concentrations in JIA showed a positive correlation with the Child Health Assessment Questionnaire (CHAQ; r=0.80) and erythrocyte sedimentation rate (r=0.45), but not with the total leucocyte count (r=0.26). Mean MRP8/MRP14 serum concentrations dropped markedly in the first three months after ASCT (p=0.0039) and clinical parameters of disease activity such as CHAQ markedly improved (p=0.0039). During a transient relapse there was an increase in MRP8/MRP14.

Conclusions: MRP8/MRP14 serum concentration can be used as a marker for disease activity in patients who receive an ASCT for refractory JIA. This indicates a role of macrophage activation in the pathogenesis of JIA. The occurrence of MAS in three patients in this study was not preceded by significant changes in MRP8/MRP14 concentration.

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Figure 1.

Figure 1

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MRP8/MRP14 serum concentration decreases after stem cell transplantation.

Figure 2.

Figure 2

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Correlation between ESR and MRP8/MRP14 concentration. BMT, bone marrow transplant. Please note the logarithmic scale of the y axis.

Figure 3.

Figure 3

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MRP8/MRP14 serum concentrations in different autoimmune diseases and healthy controls. SCT, stem cell transplant. Data are presented as means (SEM). Please note the logarithmic scale of the y axis.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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