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. 1997 Aug;65(8):3171–3179. doi: 10.1128/iai.65.8.3171-3179.1997

Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages.

T Madan 1, P Eggleton 1, U Kishore 1, P Strong 1, S S Aggrawal 1, P U Sarma 1, K B Reid 1
PMCID: PMC175448  PMID: 9234771

Abstract

To determine whether the lung surfactant proteins A (SP-A) and D (SP-D) are involved in the initial protective immunity against opportunistic pulmonary fungal infections caused by Aspergillus fumigatus, we performed a series of in vitro functional studies to see if SP-A and SP-D enhanced binding, phagocytosis, activation, and killing of A. fumigatus conidia by human alveolar macrophages and circulating neutrophils. Both SP-A and SP-D bound to carbohydrate structures on A. fumigatus conidia in a calcium-dependent manner. SP-A and SP-D were also chemoattractant and significantly enhanced agglutination and binding of conidia to alveolar macrophages and neutrophils. Furthermore, in the presence of SP-A and SP-D, the phagocytosis, oxidative burst, and killing of A. fumigatus conidia by neutrophils were significantly increased. These findings indicate that SP-A and SP-D may have an important immunological role in the early antifungal defense responses in the lung, through inhibiting infectivity of conidia by agglutination and by enhancing uptake and killing of A. fumigatus by phagocytic cells.

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Selected References

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  1. Azuma I., Kimura H., Hirao F., Tsubura E., Yamamura Y. Biochemical and immunological studies on aspergillus. II. Immunological properties of protein and polysaccharide fractions obtained from Aspergillus fumigatus. Mycopathol Mycol Appl. 1969 May 23;37(3):289–303. doi: 10.1007/BF02051363. [DOI] [PubMed] [Google Scholar]
  2. Bardana E. J., Jr The clinical spectrum of aspergillosis--part 2: classification and description of saprophytic, allergic, and invasive variants of human disease. Crit Rev Clin Lab Sci. 1981;13(2):85–159. doi: 10.3109/10408368009106445. [DOI] [PubMed] [Google Scholar]
  3. Binder R. E., Faling L. J., Pugatch R. D., Mahasaen C., Snider G. L. Chronic necrotizing pulmonary aspergillosis: a discrete clinical entity. Medicine (Baltimore) 1982 Mar;61(2):109–124. doi: 10.1097/00005792-198203000-00005. [DOI] [PubMed] [Google Scholar]
  4. Chroneos Z. C., Abdolrasulnia R., Whitsett J. A., Rice W. R., Shepherd V. L. Purification of a cell-surface receptor for surfactant protein A. J Biol Chem. 1996 Jul 5;271(27):16375–16383. doi: 10.1074/jbc.271.27.16375. [DOI] [PubMed] [Google Scholar]
  5. Eggleton P., Ghebrehiwet B., Coburn J. P., Sastry K. N., Zaner K. S., Tauber A. I. Characterization of the human neutrophil C1q receptor and functional effects of free ligand on activated neutrophils. Blood. 1994 Sep 1;84(5):1640–1649. [PubMed] [Google Scholar]
  6. Eggleton P., Wang L., Penhallow J., Crawford N., Brown K. A. Differences in oxidative response of subpopulations of neutrophils from healthy subjects and patients with rheumatoid arthritis. Ann Rheum Dis. 1995 Nov;54(11):916–923. doi: 10.1136/ard.54.11.916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hartshorn K. L., Crouch E. C., White M. R., Eggleton P., Tauber A. I., Chang D., Sastry K. Evidence for a protective role of pulmonary surfactant protein D (SP-D) against influenza A viruses. J Clin Invest. 1994 Jul;94(1):311–319. doi: 10.1172/JCI117323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hearn V. M., Sietsma J. H. Chemical and immunological analysis of the Aspergillus fumigatus cell wall. Microbiology. 1994 Apr;140(Pt 4):789–795. doi: 10.1099/00221287-140-4-789. [DOI] [PubMed] [Google Scholar]
  9. Holmskov U., Lawson P., Teisner B., Tornoe I., Willis A. C., Morgan C., Koch C., Reid K. B. Isolation and characterization of a new member of the scavenger receptor superfamily, glycoprotein-340 (gp-340), as a lung surfactant protein-D binding molecule. J Biol Chem. 1997 May 23;272(21):13743–13749. doi: 10.1074/jbc.272.21.13743. [DOI] [PubMed] [Google Scholar]
  10. Kishore U., Eggleton P., Reid K. B. Modular organization of carbohydrate recognition domains in animal lectins. Matrix Biol. 1997 Mar;15(8-9):583–592. doi: 10.1016/s0945-053x(97)90035-4. [DOI] [PubMed] [Google Scholar]
  11. Korfhagen T. R., Bruno M. D., Ross G. F., Huelsman K. M., Ikegami M., Jobe A. H., Wert S. E., Stripp B. R., Morris R. E., Glasser S. W. Altered surfactant function and structure in SP-A gene targeted mice. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9594–9599. doi: 10.1073/pnas.93.18.9594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kuan S. F., Rust K., Crouch E. Interactions of surfactant protein D with bacterial lipopolysaccharides. Surfactant protein D is an Escherichia coli-binding protein in bronchoalveolar lavage. J Clin Invest. 1992 Jul;90(1):97–106. doi: 10.1172/JCI115861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kurup V. P., Kumar A. Immunodiagnosis of aspergillosis. Clin Microbiol Rev. 1991 Oct;4(4):439–456. doi: 10.1128/cmr.4.4.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Latge J. P., Debeaupuis J. P., Sarfati J., Diaquin M., Paris S. Cell wall antigens in Aspergillus fumigatus. Arch Med Res. 1993 Autumn;24(3):269–274. [PubMed] [Google Scholar]
  15. Levitz S. M., Diamond R. D. A rapid colorimetric assay of fungal viability with the tetrazolium salt MTT. J Infect Dis. 1985 Nov;152(5):938–945. doi: 10.1093/infdis/152.5.938. [DOI] [PubMed] [Google Scholar]
  16. Lu J., Willis A. C., Reid K. B. Purification, characterization and cDNA cloning of human lung surfactant protein D. Biochem J. 1992 Jun 15;284(Pt 3):795–802. doi: 10.1042/bj2840795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miyamura K., Malhotra R., Hoppe H. J., Reid K. B., Phizackerley P. J., Macpherson P., López Bernal A. Surfactant proteins A (SP-A) and D (SP-D): levels in human amniotic fluid and localization in the fetal membranes. Biochim Biophys Acta. 1994 Jan 20;1210(3):303–307. doi: 10.1016/0005-2760(94)90233-x. [DOI] [PubMed] [Google Scholar]
  18. Reid K. B. Structure/function relationships in the collectins (mammalian lectins containing collagen-like regions). Biochem Soc Trans. 1993 May;21(2):464–468. doi: 10.1042/bst0210464. [DOI] [PubMed] [Google Scholar]
  19. Rinaldi M. G. Invasive aspergillosis. Rev Infect Dis. 1983 Nov-Dec;5(6):1061–1077. doi: 10.1093/clinids/5.6.1061. [DOI] [PubMed] [Google Scholar]
  20. Schaffner A., Douglas H., Braude A. Selective protection against conidia by mononuclear and against mycelia by polymorphonuclear phagocytes in resistance to Aspergillus. Observations on these two lines of defense in vivo and in vitro with human and mouse phagocytes. J Clin Invest. 1982 Mar;69(3):617–631. doi: 10.1172/JCI110489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schelenz S., Malhotra R., Sim R. B., Holmskov U., Bancroft G. J. Binding of host collectins to the pathogenic yeast Cryptococcus neoformans: human surfactant protein D acts as an agglutinin for acapsular yeast cells. Infect Immun. 1995 Sep;63(9):3360–3366. doi: 10.1128/iai.63.9.3360-3366.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wang J. Y., Kishore U., Lim B. L., Strong P., Reid K. B. Interaction of human lung surfactant proteins A and D with mite (Dermatophagoides pteronyssinus) allergens. Clin Exp Immunol. 1996 Nov;106(2):367–373. doi: 10.1046/j.1365-2249.1996.d01-838.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wright J. R., Youmans D. C. Pulmonary surfactant protein A stimulates chemotaxis of alveolar macrophage. Am J Physiol. 1993 Apr;264(4 Pt 1):L338–L344. doi: 10.1152/ajplung.1993.264.4.L338. [DOI] [PubMed] [Google Scholar]
  24. de Repentigny L., Petitbois S., Boushira M., Michaliszyn E., Sénéchal S., Gendron N., Montplaisir S. Acquired immunity in experimental murine aspergillosis is mediated by macrophages. Infect Immun. 1993 Sep;61(9):3791–3802. doi: 10.1128/iai.61.9.3791-3802.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. van Iwaarden F., Welmers B., Verhoef J., Haagsman H. P., van Golde L. M. Pulmonary surfactant protein A enhances the host-defense mechanism of rat alveolar macrophages. Am J Respir Cell Mol Biol. 1990 Jan;2(1):91–98. doi: 10.1165/ajrcmb/2.1.91. [DOI] [PubMed] [Google Scholar]

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