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. 1997 Aug;65(8):3474–3477. doi: 10.1128/iai.65.8.3474-3477.1997

Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model.

J A Bénitez 1, R G Spelbrink 1, A Silva 1, T E Phillips 1, C M Stanley 1, M Boesman-Finkelstein 1, R A Finkelstein 1
PMCID: PMC175493  PMID: 9234816

Abstract

Choleragenic vibrios adhered to and multiplied on monolayers of the highly differentiated mucin-secreting cell line HT29-18N2. Their adherence followed first-order kinetics, was dependent on the concentration of vibrios, and was partially inhibited by lipopolysaccharide. Comparison of genetically modified vibrios showed that flagella, an active toxR gene, and the virulence cassette were not essential for initial binding. Inactivation of the hemagglutinin/protease increased binding. This highly differentiated human intestinal cell line provides a versatile new approach for studying major events occurring during intestinal colonization: adherence, multiplication, and detachment.

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Selected References

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