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. 1997 Oct;65(10):4146–4151. doi: 10.1128/iai.65.10.4146-4151.1997

Protective efficacy of antibodies to the Staphylococcus aureus type 5 capsular polysaccharide in a modified model of endocarditis in rats.

J C Lee 1, J S Park 1, S E Shepherd 1, V Carey 1, A Fattom 1
PMCID: PMC175596  PMID: 9317020

Abstract

The protective efficacy of antibodies to the Staphylococcus aureus type 5 capsular polysaccharide (CP5) was examined in a modified model of catheter-induced endocarditis. Rats were catheterized by surgically passing a polyethylene catheter through the right carotid artery and aortic valve into the left ventricle. The following day, the rats were injected by the intraperitoneal (i.p.) route with immunoglobulin G (IgG) purified from nonimmunized rabbits or from rabbits immunized with a conjugate vaccine composed of CP5 and CP8 linked covalently to recombinant Pseudomonas aeruginosa exotoxoid A. One day after passive immunization, the animals were challenged i.p. with one of three serotype 5 S. aureus isolates (strain Reynolds, Lowenstein, or VP) or nontypeable strain 521. Protection was evaluated by comparing quantitative cultures of blood, endocardial vegetations, and kidneys from control and immune animals. For experiments performed with S. aureus Reynolds and Lowenstein, rats given capsular antibodies (645 microg of CP5-specific IgG) showed a significantly (P < 0.05) lower prevalence of endocarditis than rats injected with nonimmune IgG. Similarly, quantitative cultures of the blood, kidneys, and aortic valve vegetations revealed that fewer S. aureus cells were recovered from rats given capsule-specific IgG than from rats administered nonimmune IgG. Rats challenged with strain VP were protected with 1.145 mg of CP5-specific IgG. Capsular antibodies did not protect against infection elicited by a nontypeable strain. These results demonstrate that capsular antibodies elicited by immunization with a polysaccharide-protein conjugate vaccine protect experimental animals against serotype 5 S. aureus infection in a modified model of endocarditis.

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Selected References

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  1. Baddour L. M., Christensen G. D., Hester M. G., Bisno A. L. Production of experimental endocarditis by coagulase-negative staphylococci: variability in species virulence. J Infect Dis. 1984 Nov;150(5):721–727. doi: 10.1093/infdis/150.5.721. [DOI] [PubMed] [Google Scholar]
  2. FISHER S. A heat stable protective staphylococcal antigen. Aust J Exp Biol Med Sci. 1960 Dec;38:479–485. doi: 10.1038/icb.1960.53. [DOI] [PubMed] [Google Scholar]
  3. Fattom A. I., Sarwar J., Ortiz A., Naso R. A Staphylococcus aureus capsular polysaccharide (CP) vaccine and CP-specific antibodies protect mice against bacterial challenge. Infect Immun. 1996 May;64(5):1659–1665. doi: 10.1128/iai.64.5.1659-1665.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fattom A., Schneerson R., Szu S. C., Vann W. F., Shiloach J., Karakawa W. W., Robbins J. B. Synthesis and immunologic properties in mice of vaccines composed of Staphylococcus aureus type 5 and type 8 capsular polysaccharides conjugated to Pseudomonas aeruginosa exotoxin A. Infect Immun. 1990 Jul;58(7):2367–2374. doi: 10.1128/iai.58.7.2367-2374.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hienz S. A., Schennings T., Heimdahl A., Flock J. I. Collagen binding of Staphylococcus aureus is a virulence factor in experimental endocarditis. J Infect Dis. 1996 Jul;174(1):83–88. doi: 10.1093/infdis/174.1.83. [DOI] [PubMed] [Google Scholar]
  6. Kuypers J. M., Proctor R. A. Reduced adherence to traumatized rat heart valves by a low-fibronectin-binding mutant of Staphylococcus aureus. Infect Immun. 1989 Aug;57(8):2306–2312. doi: 10.1128/iai.57.8.2306-2312.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee J. C., Perez N. E., Hopkins C. A., Pier G. B. Purified capsular polysaccharide-induced immunity to Staphylococcus aureus infection. J Infect Dis. 1988 Apr;157(4):723–730. doi: 10.1093/infdis/157.4.723. [DOI] [PubMed] [Google Scholar]
  8. Lee J. C. The prospects for developing a vaccine against Staphylococcus aureus. Trends Microbiol. 1996 Apr;4(4):162–166. doi: 10.1016/0966-842x(96)10021-4. [DOI] [PubMed] [Google Scholar]
  9. Moreillon P., Entenza J. M., Francioli P., McDevitt D., Foster T. J., François P., Vaudaux P. Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis. Infect Immun. 1995 Dec;63(12):4738–4743. doi: 10.1128/iai.63.12.4738-4743.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nemeth J., Lee J. C. Antibodies to capsular polysaccharides are not protective against experimental Staphylococcus aureus endocarditis. Infect Immun. 1995 Feb;63(2):375–380. doi: 10.1128/iai.63.2.375-380.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Santoro J., Levison M. E. Rat model of experimental endocarditis. Infect Immun. 1978 Mar;19(3):915–918. doi: 10.1128/iai.19.3.915-918.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schennings T., Heimdahl A., Coster K., Flock J. I. Immunization with fibronectin binding protein from Staphylococcus aureus protects against experimental endocarditis in rats. Microb Pathog. 1993 Sep;15(3):227–236. doi: 10.1006/mpat.1993.1073. [DOI] [PubMed] [Google Scholar]
  13. Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol. 1985 Nov;22(5):828–834. doi: 10.1128/jcm.22.5.828-834.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Xu S., Arbeit R. D., Lee J. C. Phagocytic killing of encapsulated and microencapsulated Staphylococcus aureus by human polymorphonuclear leukocytes. Infect Immun. 1992 Apr;60(4):1358–1362. doi: 10.1128/iai.60.4.1358-1362.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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