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. 1997 Oct;65(10):4337–4340. doi: 10.1128/iai.65.10.4337-4340.1997

Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages.

P A Denoel 1, R M Crawford 1, M S Zygmunt 1, A Tibor 1, V E Weynants 1, F Godfroid 1, D L Hoover 1, J J Letesson 1
PMCID: PMC175622  PMID: 9317046

Abstract

A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.

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Selected References

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