Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1997 Nov;65(11):4711–4717. doi: 10.1128/iai.65.11.4711-4717.1997

Differences in coughing and other responses to intrabronchial infection with Bordetella pertussis among strains of rats.

E Hall 1, R Parton 1, A C Wardlaw 1
PMCID: PMC175676  PMID: 9353055

Abstract

Four strains of rats were each infected intrabronchially with approximately 10(8) CFU of Bordetella pertussis 18-323 encased in fine agarose beads. After 8 days, Sprague-Dawley rats developed the highest incidence of coughing paroxysms, as monitored with voice-activated tape recorders; Brown Norway, Lewis, and Hooded Lister rats coughed significantly less frequently. Marked leukocytosis, with counts up to four times the normal levels, and retardation of normal weight gain occurred in all four rat strains. Both coughing and leukocytosis were greater in animals that were infected at 4 weeks of age than in those infected at 6 weeks of age. Total serum immunoglobulin E (IgE) rose in all four rat strains 9- to 244-fold by day 8 after infection and returned to near preinfection levels at 6 weeks. Sprague-Dawley and Lewis rats, which had the lowest basal levels of total IgE in serum, showed the greatest degrees of elevation. All four rat strains had IgG to B. pertussis whole-cell sonicate and to filamentous hemagglutinin in 6-week-postinfection sera. However, the strains differed in production of IgG to pertussis toxin, with Sprague-Dawley rats having the highest titers and Hooded Lister and Lewis rats being nonresponders. These studies highlight the importance of rat strain as a variable in the coughing-rat model of pertussis and validate the choice of the Sprague-Dawley rats in previous studies.

Full Text

The Full Text of this article is available as a PDF (796.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coffman R. L., Lebman D. A., Rothman P. Mechanism and regulation of immunoglobulin isotype switching. Adv Immunol. 1993;54:229–270. doi: 10.1016/s0065-2776(08)60536-2. [DOI] [PubMed] [Google Scholar]
  2. Hall E. P., Gault E. A. Suppression of IgE responses by hyperimmune serum in rats. Immunology. 1986 Dec;59(4):577–581. [PMC free article] [PubMed] [Google Scholar]
  3. Hall E., Parton R., Wardlaw A. C. Cough production, leucocytosis and serology of rats infected intrabronchially with Bordetella pertussis. J Med Microbiol. 1994 Mar;40(3):205–213. doi: 10.1099/00222615-40-3-205. [DOI] [PubMed] [Google Scholar]
  4. Haus M., Weinberg E. G., Malherbe D. Specific IgE antibodies to Bordetella pertussis after immunisation in infancy. Lancet. 1988 Mar 26;1(8587):711–711. doi: 10.1016/s0140-6736(88)91518-8. [DOI] [PubMed] [Google Scholar]
  5. Hedenskog S., Björkstén B., Blennow M., Granström G., Granström M. Immunoglobulin E response to pertussis toxin in whooping cough and after immunization with a whole-cell and an acellular pertussis vaccine. Int Arch Allergy Appl Immunol. 1989;89(2-3):156–161. doi: 10.1159/000234939. [DOI] [PubMed] [Google Scholar]
  6. Heininger U., Stehr K., Schmitt-Grohé S., Lorenz C., Rost R., Christenson P. D., Uberall M., Cherry J. D. Clinical characteristics of illness caused by Bordetella parapertussis compared with illness caused by Bordetella pertussis. Pediatr Infect Dis J. 1994 Apr;13(4):306–309. doi: 10.1097/00006454-199404000-00011. [DOI] [PubMed] [Google Scholar]
  7. Hirashima M., Yodoi J., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. V. formation of IgE-potentiating factor by T lymphocytes from rats treated with Bordetella pertussis vaccine. J Immunol. 1981 Mar;126(3):838–842. [PubMed] [Google Scholar]
  8. Hozbor D., Rodriguez M. E., Yantorno O. Use of cyclodextrin as an agent to induce excretion of Bordetella pertussis antigens. FEMS Immunol Med Microbiol. 1994 Aug;9(2):117–124. doi: 10.1111/j.1574-695X.1994.tb00482.x. [DOI] [PubMed] [Google Scholar]
  9. Ishizaka K. Regulation of immunoglobin E biosynthesis. Adv Immunol. 1989;47:1–44. [PubMed] [Google Scholar]
  10. Lindsay D. S., Parton R., Wardlaw A. C. Adjuvant effect of pertussis toxin on the production of anti-ovalbumin IgE in mice and lack of direct correlation between PCA and ELISA. Int Arch Allergy Immunol. 1994 Nov;105(3):281–288. doi: 10.1159/000236770. [DOI] [PubMed] [Google Scholar]
  11. MORSE S. I. STUDIES ON THE LYMPHOCYTOSIS INDUCED IN MICE BY BORDETELLA PERTUSSIS. J Exp Med. 1965 Jan 1;121:49–68. doi: 10.1084/jem.121.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mu H. H., Sewell W. A. Regulation of DTH and IgE responses by IL-4 and IFN-gamma in immunized mice given pertussis toxin. Immunology. 1994 Dec;83(4):639–645. [PMC free article] [PubMed] [Google Scholar]
  13. Munoz J. J., Arai H., Bergman R. K., Sadowski P. L. Biological activities of crystalline pertussigen from Bordetella pertussis. Infect Immun. 1981 Sep;33(3):820–826. doi: 10.1128/iai.33.3.820-826.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Olson L. C. Pertussis. Medicine (Baltimore) 1975 Nov;54(6):427–469. doi: 10.1097/00005792-197511000-00001. [DOI] [PubMed] [Google Scholar]
  15. Parton R., Hall E., Wardlaw A. C. Responses to Bordetella pertussis mutant strains and to vaccination in the coughing rat model of pertussis. J Med Microbiol. 1994 May;40(5):307–312. doi: 10.1099/00222615-40-5-307. [DOI] [PubMed] [Google Scholar]
  16. Pittman M. The concept of pertussis as a toxin-mediated disease. Pediatr Infect Dis. 1984 Sep-Oct;3(5):467–486. doi: 10.1097/00006454-198409000-00019. [DOI] [PubMed] [Google Scholar]
  17. Tada T., Okumura K., Ochiai T., Iwasa S. Effect of lymphocytosis-promoting factor of Bordetella pertussis on the immune response. II. Adjuvant effect for the production of reaginic antibody in the rat. Int Arch Allergy Appl Immunol. 1972;43(2):207–216. doi: 10.1159/000230838. [DOI] [PubMed] [Google Scholar]
  18. Torre D., Issi M., Chelazzi G., Fiori G. P., Sampietro C. Total serum IgE levels in children with pertussis. Am J Dis Child. 1990 Mar;144(3):290–291. doi: 10.1001/archpedi.1990.02150270040022. [DOI] [PubMed] [Google Scholar]
  19. Wirsing von Koenig C. H., Finger H. Specific IgE-antibodies to Bordetella pertussis antigens during whooping cough. Lancet. 1989 Apr 1;1(8640):728–728. doi: 10.1016/s0140-6736(89)92245-9. [DOI] [PubMed] [Google Scholar]
  20. Wirsing von König C. H., Finger H. Role of pertussis toxin in causing symptoms of Bordetella parapertussis infection. Eur J Clin Microbiol Infect Dis. 1994 Jun;13(6):455–458. doi: 10.1007/BF01974634. [DOI] [PubMed] [Google Scholar]
  21. Woods D. E., Franklin R., Cryz S. J., Jr, Ganss M., Peppler M., Ewanowich C. Development of a rat model for respiratory infection with Bordetella pertussis. Infect Immun. 1989 Apr;57(4):1018–1024. doi: 10.1128/iai.57.4.1018-1024.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES