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. 1997 Nov;65(11):4806–4812. doi: 10.1128/iai.65.11.4806-4812.1997

Invasion of intestinal epithelia in vitro by the parasitic nematode Trichinella spiralis.

T ManWarren 1, L Gagliardo 1, J Geyer 1, C McVay 1, S Pearce-Kelling 1, J Appleton 1
PMCID: PMC175690  PMID: 9353069

Abstract

Studies of nematode establishment in intestinal niches has been hindered by the lack of a readily manipulated in vitro assay. In this report, experiments are described wherein the larval stage of the parasitic nematode Trichinella spiralis was shown to invade epithelial cell monolayers in vitro. Larvae penetrated cells and migrated through them, leaving trails of dead cells in their wake. Cells derived from five different species were susceptible to invasion, reflecting the broad host range of T. spiralis in vivo. Epithelial cells derived from large and small intestines and kidneys were susceptible. Fibroblast and muscle cells were resistant. Larvae deposited glycoprotein antigens in the cells they invaded. Although the function of these antigens is unknown, they are targeted by rat antibodies that cause T. spiralis to be expelled from the intestine. The model system described provides the means to further investigate this process as well as the mechanisms by which this parasitic nematode establishes its intestinal niche.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleton J. A., McGregor D. D. Characterization of the immune mediator of rapid expulsion of Trichinella spiralis in suckling rats. Immunology. 1987 Nov;62(3):477–484. [PMC free article] [PubMed] [Google Scholar]
  2. Appleton J. A., McGregor D. D. Rapid expulsion of Trichinella spiralis in suckling rats. Science. 1984 Oct 5;226(4670):70–72. doi: 10.1126/science.6474191. [DOI] [PubMed] [Google Scholar]
  3. Appleton J. A., Schain L. R., McGregor D. D. Rapid expulsion of Trichinella spiralis in suckling rats: mediation by monoclonal antibodies. Immunology. 1988 Nov;65(3):487–492. [PMC free article] [PubMed] [Google Scholar]
  4. Arasu P., Ellis L. A., Iglesias R., Ubeira F. M., Appleton J. A. Molecular analysis of antigens targeted by protective antibodies in rapid expulsion of Trichinella spiralis. Mol Biochem Parasitol. 1994 Jun;65(2):201–211. doi: 10.1016/0166-6851(94)90072-8. [DOI] [PubMed] [Google Scholar]
  5. Bell R. G., McGregor D. D. Trichinella spiralis: expression of rapid expulsion in rats exposed to an abbreviated enteral infection. Exp Parasitol. 1979 Aug;48(1):42–50. doi: 10.1016/0014-4894(79)90053-5. [DOI] [PubMed] [Google Scholar]
  6. Castro G. A., Badial-Aceves F., Adams P. R., Copeland E. M., Dudrick S. J. Response to immunized, parenterally nourished rats to challenge infection with the nematode, Trichinella spiralis. J Nutr. 1976 Oct;106(10):1484–1491. doi: 10.1093/jn/106.10.1484. [DOI] [PubMed] [Google Scholar]
  7. Crum E. D., Despommier D. D., McGregor D. D. Immunity to Trichinella spiralis. I. Transfer of resistance by two classes of lymphocytes. Immunology. 1977 Dec;33(6):787–795. [PMC free article] [PubMed] [Google Scholar]
  8. Drake L., Korchev Y., Bashford L., Djamgoz M., Wakelin D., Ashall F., Bundy D. The major secreted product of the whipworm, Trichuris, is a pore-forming protein. Proc Biol Sci. 1994 Sep 22;257(1350):255–261. doi: 10.1098/rspb.1994.0123. [DOI] [PubMed] [Google Scholar]
  9. Dunn I. J., Wright K. A. The response of the intestinal epithelium in B10.A mice to infection with Trichinella spiralis. J Parasitol. 1987 Aug;73(4):712–722. [PubMed] [Google Scholar]
  10. Ellis L. A., Reason A. J., Morris H. R., Dell A., Iglesias R., Ubeira F. M., Appleton J. A. Glycans as targets for monoclonal antibodies that protect rats against Trichinella spiralis. Glycobiology. 1994 Oct;4(5):585–592. doi: 10.1093/glycob/4.5.585. [DOI] [PubMed] [Google Scholar]
  11. Lee T. D., Wright K. A. The morphology of the attachment and probable feeding site of the nematode Trichuris muris (Schrank, 1788) Hall, 1916. Can J Zool. 1978 Sep;56(9):1889–1905. doi: 10.1139/z78-258. [DOI] [PubMed] [Google Scholar]
  12. Love R. J., Ogilvie B. M., McLaren D. J. The immune mechanism which expels the intestinal stage of Trichinella spiralis from rats. Immunology. 1976 Jan;30(1):7–15. [PMC free article] [PubMed] [Google Scholar]
  13. McLaren D. J. Nematode sense organs. Adv Parasitol. 1976;14:195–265. doi: 10.1016/s0065-308x(08)60515-1. [DOI] [PubMed] [Google Scholar]
  14. Otubu O. E., Carlisle-Nowak M. S., McGregor D. D., Jacobson R. H., Appleton J. A. Trichinella spiralis: the effect of specific antibody on muscle larvae in the small intestines of weaned rats. Exp Parasitol. 1993 Jun;76(4):394–400. doi: 10.1006/expr.1993.1048. [DOI] [PubMed] [Google Scholar]
  15. Stewart G. L., Despommier D. D., Burnham J., Raines K. M. Trichinella spiralis: behavior, structure, and biochemistry of larvae following exposure to components of the host enteric environment. Exp Parasitol. 1987 Apr;63(2):195–204. doi: 10.1016/0014-4894(87)90162-7. [DOI] [PubMed] [Google Scholar]
  16. Su X. Z., Prestwood A. K., McGraw R. A. Cloning and expression of complementary DNA encoding an antigen of Trichinella spiralis. Mol Biochem Parasitol. 1991 Apr;45(2):331–336. doi: 10.1016/0166-6851(91)90101-b. [DOI] [PubMed] [Google Scholar]
  17. Vassilatis D. K., Despommier D., Misek D. E., Polvere R. I., Gold A. M., Van der Ploeg L. H. Analysis of a 43-kDa glycoprotein from the intracellular parasitic nematode Trichinella spiralis. J Biol Chem. 1992 Sep 15;267(26):18459–18465. [PubMed] [Google Scholar]
  18. Wakelin D., Lloyd M. Immunity to primary and challenge infections of Trichinella spiralis in mice: a re-examination of conventional parameters. Parasitology. 1976 Apr;72(2):173–182. doi: 10.1017/s0031182000048472. [DOI] [PubMed] [Google Scholar]
  19. Wright K. A. Trichinella spiralis: an intracellular parasite in the intestinal phase. J Parasitol. 1979 Jun;65(3):441–445. [PubMed] [Google Scholar]
  20. Wright K. A., Weidman E., Hong H. The distribution of cells killed by Trichinella spiralis in the mucosal epithelium of two strains of mice. J Parasitol. 1987 Oct;73(5):935–939. [PubMed] [Google Scholar]
  21. Zarlenga D. S., Gamble H. R. Molecular cloning and expression of an immunodominant 53-kDa excretory-secretory antigen from Trichinella spiralis muscle larvae. Mol Biochem Parasitol. 1990 Sep-Oct;42(2):165–174. doi: 10.1016/0166-6851(90)90159-j. [DOI] [PubMed] [Google Scholar]

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