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. 1997 Dec;65(12):5376–5380. doi: 10.1128/iai.65.12.5376-5380.1997

Quantification of conserved antigens in Helicobacter pylori during different culture conditions.

C Lindholm 1, J Osek 1, A M Svennerholm 1
PMCID: PMC175778  PMID: 9393845

Abstract

In this study, we raised monoclonal antibodies (MAbs) against three conserved Helicobacter pylori antigens, i.e., the N-acetylneuraminyllactose-binding fibrillar hemagglutinin, HpaA; the flagellin subunits, FlaA and FlaB; and a species-specific 26-kDa protein. The MAbs were used for the development of sensitive inhibition enzyme-linked immunosorbent assays for quantification of these antigens in H. pylori during various culture conditions. The quantities of these antigens varied considerably (up to 8-fold) during different culture procedures and between strains (up to 10-fold).

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Selected References

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