Abstract
BACKGROUND: Endothelin-1 (ET-1) is a potent bronchoconstrictor which may have a role in the pathogenesis of asthma. The levels of ET-1 in saliva, induced sputum, and plasma from asthmatic and non-asthmatic subjects were compared. METHODS: Sputum induction was performed on 28 asthmatic subjects and nine normal volunteers. ET-1 levels were measured in plasma, saliva, and sputum samples and reversed phase high performance liquid chromatography (RP-HPLC) was performed on saliva and sputum samples. RESULTS: ET-1 was present in the following order of concentration in both normal and asthmatic subjects: saliva > sputum > plasma (saliva, median 30.1 and 23.9 pg/ ml, respectively; sputum, median 15.5 and 11.2 pg/ml; plasma, median 3.1 and 3.6 pg/ ml). There were no differences between asthmatic and normal subjects in the levels of ET-1 in each fluid. The levels of ET-1 in asthmatic subjects were not influenced by whether or not they were taking inhaled steroids. RP- HPLC of sputum and saliva confirmed the presence of ET-1 in these fluids. CONCLUSIONS: Levels of ET-1 can be measured in saliva and sputum obtained by sputum induction in asthmatic and healthy subjects and, although no difference was found in basal levels of ET-1 in sputum, saliva and plasma between normal subjects and asthmatics without bronchoconstriction, it is apparent that ET-1 is produced or released locally within the respiratory tract in concentrations higher than those in plasma.
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Selected References
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- Lam H. C., Takahashi K., Ghatei M. A., Warrens A. N., Rees A. J., Bloom S. R. Immunoreactive endothelin in human plasma, urine, milk, and saliva. J Cardiovasc Pharmacol. 1991;17 (Suppl 7):S390–S393. doi: 10.1097/00005344-199100177-00109. [DOI] [PubMed] [Google Scholar]
- Maestrelli P., Saetta M., Di Stefano A., Calcagni P. G., Turato G., Ruggieri M. P., Roggeri A., Mapp C. E., Fabbri L. M. Comparison of leukocyte counts in sputum, bronchial biopsies, and bronchoalveolar lavage. Am J Respir Crit Care Med. 1995 Dec;152(6 Pt 1):1926–1931. doi: 10.1164/ajrccm.152.6.8520757. [DOI] [PubMed] [Google Scholar]
- Mattoli S., Soloperto M., Marini M., Fasoli A. Levels of endothelin in the bronchoalveolar lavage fluid of patients with symptomatic asthma and reversible airflow obstruction. J Allergy Clin Immunol. 1991 Sep;88(3 Pt 1):376–384. doi: 10.1016/0091-6749(91)90100-3. [DOI] [PubMed] [Google Scholar]
- Pizzichini E., Pizzichini M. M., Efthimiadis A., Evans S., Morris M. M., Squillace D., Gleich G. J., Dolovich J., Hargreave F. E. Indices of airway inflammation in induced sputum: reproducibility and validity of cell and fluid-phase measurements. Am J Respir Crit Care Med. 1996 Aug;154(2 Pt 1):308–317. doi: 10.1164/ajrccm.154.2.8756799. [DOI] [PubMed] [Google Scholar]
- Redington A. E., Springall D. R., Ghatei M. A., Lau L. C., Bloom S. R., Holgate S. T., Polak J. M., Howarth P. H. Endothelin in bronchoalveolar lavage fluid and its relation to airflow obstruction in asthma. Am J Respir Crit Care Med. 1995 Apr;151(4):1034–1039. doi: 10.1164/ajrccm.151.4.7697227. [DOI] [PubMed] [Google Scholar]
- Vittori E., Marini M., Fasoli A., De Franchis R., Mattoli S. Increased expression of endothelin in bronchial epithelial cells of asthmatic patients and effect of corticosteroids. Am Rev Respir Dis. 1992 Nov;146(5 Pt 1):1320–1325. doi: 10.1164/ajrccm/146.5_Pt_1.1320. [DOI] [PubMed] [Google Scholar]