Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1990 Jul;34(7):1435–1439. doi: 10.1128/aac.34.7.1435

In situ enzyme-linked immunosorbent assay to quantitate in vitro development of Eimeria tenella.

J A Olson 1
PMCID: PMC175995  PMID: 2201258

Abstract

An in situ enzyme-linked immunosorbent assay was developed to measure in vitro development of Eimeria tenella. The assay used a polyclonal, anti-merozoite serum produced by immunization with culture-derived, chromatographically purified merozoites. Although this antiserum cross-reacted with sporozoite-infected cultures (by indirect immunofluorescence and in situ enzyme-linked immunosorbent assay), it clearly distinguished the increase in antigen synthesized throughout intracellular growth. The assay can be used for high-throughput, anticoccidial drug screening, for which it gives quantitative results that are comparable to the published radiometric [( 3H]uracil incorporation) endpoint.

Full text

PDF
1435

Images in this article

1437Image
on p.1437
1438Image
on p.1438

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. André P. M., Narbonne C. H., Donnio P. Y., Ruffault A., Fauconnier B. Evaluation of herpes simplex virus susceptibility to acyclovir using an enzyme-linked immunosorbent assay. Ann Inst Pasteur Virol. 1988 Apr-Jun;139(2):185–195. doi: 10.1016/s0769-2617(88)80016-9. [DOI] [PubMed] [Google Scholar]
  2. Berkowitz F. E., Levin M. J. Use of an enzyme-linked immunosorbent assay performed directly on fixed infected cell monolayers for evaluating drugs against varicella-zoster virus. Antimicrob Agents Chemother. 1985 Aug;28(2):207–210. doi: 10.1128/aac.28.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlson P. L. Adaptation of enzyme-linked immunoassay for detection of avian and murine antibodies to Eimeria. Avian Dis. 1984 Oct-Dec;28(4):1115–1119. [PubMed] [Google Scholar]
  4. Danforth H. D., McAndrew S. J. Hybridoma antibody characterization of stage-specific and stage-cross-reactive antigens of Eimeria tenella. J Parasitol. 1987 Oct;73(5):985–992. [PubMed] [Google Scholar]
  5. De Titto E. H., Araujo F. G. Enzyme immunoassay in cell monolayers for evaluation of in vitro activity of chemotherapeutic agents against Trypanosoma cruzi. Am J Trop Med Hyg. 1988 Sep;39(3):274–281. doi: 10.4269/ajtmh.1988.39.274. [DOI] [PubMed] [Google Scholar]
  6. Derouin F., Chastang C. Enzyme immunoassay to assess effect of antimicrobial agents on Toxoplasma gondii in tissue culture. Antimicrob Agents Chemother. 1988 Mar;32(3):303–307. doi: 10.1128/aac.32.3.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feit C., Bartal A. H., Tauber G., Dymbort G., Hirshaut Y. An enzyme-linked immunosorbent assay (ELISA) for the detection of monoclonal antibodies recognizing surface antigens expressed on viable cells. J Immunol Methods. 1983 Mar 25;58(3):301–308. doi: 10.1016/0022-1759(83)90358-7. [DOI] [PubMed] [Google Scholar]
  8. Gilbert J. M., Bhanushali J. K., McDougald L. R. An enzyme-linked immunosorbent assay for coccidiosis in chickens: correlation of antibody levels with prior exposure to coccidia in the laboratory and in the field. Avian Dis. 1988 Oct-Dec;32(4):688–694. [PubMed] [Google Scholar]
  9. Hollingdale M. R., Kilejian A. Purification of Plasmodium lophurae exoerythrocytic merozoites by an ion exchange column. J Protozool. 1979 Nov;26(4):616–619. doi: 10.1111/j.1550-7408.1979.tb04207.x. [DOI] [PubMed] [Google Scholar]
  10. Kang J. O., Pai C. H. In situ enzyme immunoassay for antiviral susceptibility testing of respiratory syncytial virus. Am J Clin Pathol. 1989 Mar;91(3):323–326. doi: 10.1093/ajcp/91.3.323. [DOI] [PubMed] [Google Scholar]
  11. Kilejian A. A unique histidine-rich polypeptide from the malaria parasite, Plasmodium lophurae. J Biol Chem. 1974 Jul 25;249(14):4650–4655. [PubMed] [Google Scholar]
  12. Merli A., Canessa A., Melioli G. Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture. J Clin Microbiol. 1985 Jan;21(1):88–91. doi: 10.1128/jcm.21.1.88-91.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mirabelli C. K., Bartus H., Bartus J. O., Johnson R., Mong S. M., Sung C. P., Crooke S. T. Application of a tissue culture microtiter test for the detection of cytotoxic agents from natural products. J Antibiot (Tokyo) 1985 Jun;38(6):758–766. doi: 10.7164/antibiotics.38.758. [DOI] [PubMed] [Google Scholar]
  14. Nilheden E., Jeansson S., Vahlne A. Typing of herpes simplex virus by an enzyme-linked immunosorbent assay with monoclonal antibodies. J Clin Microbiol. 1983 Apr;17(4):677–680. doi: 10.1128/jcm.17.4.677-680.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Onaga H., Saeki H., Hoshi S., Ueda S. An enzyme-linked immunosorbent assay for serodiagnosis of coccidiosis in chickens: use of a single serum dilution. Avian Dis. 1986 Oct-Dec;30(4):658–661. [PubMed] [Google Scholar]
  16. Patton W. H. Eimeria tenella: cultivation of the asexual stages in cultured animal cells. Science. 1965 Nov 5;150(3697):767–769. doi: 10.1126/science.150.3697.767. [DOI] [PubMed] [Google Scholar]
  17. Posner M. R., Antoniou D., Griffin J., Schlossman S. F., Lazarus H. An enzyme-linked immunosorbent assay (ELISA) for the detection of monoclonal antibodies to cell surface antigens on viable cells. J Immunol Methods. 1982;48(1):23–31. doi: 10.1016/0022-1759(82)90206-x. [DOI] [PubMed] [Google Scholar]
  18. Rabalais G. P., Levin M. J., Berkowitz F. E. Rapid herpes simplex virus susceptibility testing using an enzyme-linked immunosorbent assay performed in situ on fixed virus-infected monolayers. Antimicrob Agents Chemother. 1987 Jun;31(6):946–948. doi: 10.1128/aac.31.6.946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rose M. E., Mockett A. P. Antibodies to coccidia: detection by the enzyme-linked immunosorbent assay (ELISA). Parasite Immunol. 1983 Sep;5(5):479–489. doi: 10.1111/j.1365-3024.1983.tb00762.x. [DOI] [PubMed] [Google Scholar]
  20. Saatara Oz H., Markham R. J., Bemrick W. J., Stromberg B. E. Enzyme-linked immunosorbent assay and indirect haemagglutination techniques for measurement of antibody responses to Eimeria tenella in experimentally infected chickens. J Parasitol. 1984 Dec;70(6):859–863. [PubMed] [Google Scholar]
  21. Schmatz D. M., Crane M. S., Murray P. K. Eimeria tenella: parasite-specific incorporation of 3H-uracil as a quantitative measure of intracellular development. J Protozool. 1986 Feb;33(1):109–114. doi: 10.1111/j.1550-7408.1986.tb05568.x. [DOI] [PubMed] [Google Scholar]
  22. Schmatz D. M., Crane M. S., Murray P. K. Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. J Protozool. 1984 Feb;31(1):181–183. doi: 10.1111/j.1550-7408.1984.tb04314.x. [DOI] [PubMed] [Google Scholar]
  23. Strout R. G., Quellette C. A. Eimeria tenella: screening of chemotherapeutic compounds in cell cultures. Exp Parasitol. 1973 Jun;33(3):477–485. doi: 10.1016/0014-4894(73)90115-x. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES