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. 1988 Dec;32(12):1797–1800. doi: 10.1128/aac.32.12.1797

The cryptic tetracycline resistance determinant on Tn4400 mediates tetracycline degradation as well as tetracycline efflux.

B H Park 1, S B Levy 1
PMCID: PMC176021  PMID: 3072922

Abstract

Escherichia coli containing the cryptic tetracycline resistance determinant (class F) from the Bacteroides fragilis transposon Tn4400 on plasmid pGAT400 expressed a detoxification of tetracycline as well as an active efflux of tetracycline. This finding concurs with the report of detoxification for a related tetracycline resistance determinant from B. fragilis on Tn4351 (B. S. Speer and A. Salyers, J. Bacteriol. 170:1423-1429, 1987), which specifies a 10-fold-higher resistance than Tn4400. Inactivation of tetracycline occurred at an initial rate of congruent to 0.7 micrograms of tetracycline per h per 10(8) cells, as determined by biologic assay and chromatographic analysis. The detoxification is a chemical degradation which can occur in the absence of energy-dependent efflux. The products of this degradation were not substrates for active transport into susceptible cells or out of pGAT400-containing E. coli. These results indicate that Tn4400 mediates two functionally different mechanisms for tetracycline resistance: an active efflux of tetracycline and a degradation of tetracycline.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Guiney D. G., Hasegawa P., Davis C. E. Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7203–7206. doi: 10.1073/pnas.81.22.7203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Guiney D. G., Jr, Hasegawa P., Davis C. E. Expression in Escherichia coli of cryptic tetracycline resistance genes from bacteroides R plasmids. Plasmid. 1984 May;11(3):248–252. doi: 10.1016/0147-619x(84)90031-3. [DOI] [PubMed] [Google Scholar]
  3. Guiney D. G., Jr, Hasegawa P., Davis C. E. Homology between clindamycin resistance plasmids in Bacteroides. Plasmid. 1984 May;11(3):268–271. doi: 10.1016/0147-619x(84)90035-0. [DOI] [PubMed] [Google Scholar]
  4. Guiney D. G., Yakobson E. Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3595–3598. doi: 10.1073/pnas.80.12.3595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984 Jul;12(1):19–36. doi: 10.1016/0147-619x(84)90063-5. [DOI] [PubMed] [Google Scholar]
  6. Levy S. B., McMurry L. Detection of an inducible membrane protein associated with R-factor-mediated tetracycline resistance. Biochem Biophys Res Commun. 1974 Feb 27;56(4):1060–1068. doi: 10.1016/s0006-291x(74)80296-2. [DOI] [PubMed] [Google Scholar]
  7. Matthews B. G., Guiney D. G. Characterization and mapping of regions encoding clindamycin resistance, tetracycline resistance, and a replication function on the Bacteroides R plasmid pCP1. J Bacteriol. 1986 Aug;167(2):517–521. doi: 10.1128/jb.167.2.517-521.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McMurry L. M., Park B. H., Burdett V., Levy S. B. Energy-dependent efflux mediated by class L (tetL) tetracycline resistance determinant from streptococci. Antimicrob Agents Chemother. 1987 Oct;31(10):1648–1650. doi: 10.1128/aac.31.10.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McMurry L., Levy S. B. Two transport systems for tetracycline in sensitive Escherichia coli: critical role for an initial rapid uptake system insensitive to energy inhibitors. Antimicrob Agents Chemother. 1978 Aug;14(2):201–209. doi: 10.1128/aac.14.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McMurry L., Petrucci R. E., Jr, Levy S. B. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974–3977. doi: 10.1073/pnas.77.7.3974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Park B. H., Hendricks M., Malamy M. H., Tally F. P., Levy S. B. Cryptic tetracycline resistance determinant (class F) from Bacteroides fragilis mediates resistance in Escherichia coli by actively reducing tetracycline accumulation. Antimicrob Agents Chemother. 1987 Nov;31(11):1739–1743. doi: 10.1128/aac.31.11.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robillard N. J., Tally F. P., Malamy M. H. Tn4400, a compound transposon isolated from Bacteroides fragilis, functions in Escherichia coli. J Bacteriol. 1985 Dec;164(3):1248–1255. doi: 10.1128/jb.164.3.1248-1255.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith C. J., Gonda M. A. Comparison of the transposon-like structures encoding clindamycin resistance in Bacteroides R-plasmids. Plasmid. 1985 May;13(3):182–192. doi: 10.1016/0147-619x(85)90041-1. [DOI] [PubMed] [Google Scholar]
  14. Speer B. S., Salyers A. A. Characterization of a novel tetracycline resistance that functions only in aerobically grown Escherichia coli. J Bacteriol. 1988 Apr;170(4):1423–1429. doi: 10.1128/jb.170.4.1423-1429.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tally F. P., Snydman D. R., Shimell M. J., Malamy M. H. Characterization of pBFTM10, a clindamycin-erythromycin resistance transfer factor from Bacteroides fragilis. J Bacteriol. 1982 Aug;151(2):686–691. doi: 10.1128/jb.151.2.686-691.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Welch R. A., Jones K. R., Macrina F. L. Transferable lincosamide-macrolide resistance in Bacteroides. Plasmid. 1979 Apr;2(2):261–268. doi: 10.1016/0147-619x(79)90044-1. [DOI] [PubMed] [Google Scholar]
  17. Welch R. A., Macrina F. L. Physical characterization of Bacteroides fragilis R plasmid pBF4. J Bacteriol. 1981 Feb;145(2):867–872. doi: 10.1128/jb.145.2.867-872.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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