Abstract
OBJECTIVE—To evaluate the in vivo biocompatibility of three different devices following interventional closure of a patent ductus arteriosus (PDA) in an animal model. MATERIALS AND METHODS—A medical grade stainless steel coil (n = 8), a nickel/titanium coil (n = 10), and a polyvinylalcohol foam plug knitted on a titanium wire frame (n = 11) were used for interventional closure of PDA in a neonatal lamb model. The PDA had been maintained by repetitive angioplasty. Between one and 278 days after implantation the animals were killed and the ductal block removed. In addition to standard histology and scanning electron microscopy, immunohistochemical staining for biocompatibility screening was also undertaken. RESULTS—Electron microscopy revealed the growth of a cellular layer in a cobblestone pattern on the implant surfaces with blood contact, which was completed as early as five weeks after implantation of all devices. Immunohistochemical staining of these superficial cells showed an endothelial cell phenotype. After initial thrombus formation causing occlusion of the PDA after implantation there was ingrowth of fibromuscular cells resembling smooth muscle cells. Transformation of thrombotic material was completed within six weeks in the polyvinylalcohol plug and around the nickel/titanium coil, and within six months after implantation of the stainless steel coil. An implant related foreign body reaction was seen in only one of the stainless steel coil specimens and in two of the nickel/titanium coil specimens. CONCLUSION—After implantation, organisation of thrombotic material with ingrowth of fibromuscular cells was demonstrated in a material dependent time pattern. The time it took for endothelium to cover the implants was independent of the type of implant. Little or no inflammatory reaction of the surrounding tissue was seen nine months after implantation. Keywords: congenital heart disease; patent ductus arteriosus; catheter technique; biocompatibility
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Figure 1 .
The implants. Top, foam polyvinylalcohol plug; bottom left, stainless steel coil; bottom right, nickel/titanium coil.
Figure 2 .
Cobblestone pattern of endothelial cells on the surface of the aortic orifice of a ductus arteriosus 112 days after interventional closure with a polyvinylalcohol plug (lamb 6). Electron microscopy × 600.
Figure 3 .
Positive immunohistochemical staining for von Willebrand factor in superficial endothelial cells (smaller arrows) covering the aortic orifice of a ductus arteriosus 117 days after implantation of a polyvinylalcohol plug. Larger arrows indicate foam material. × 800.
Figure 4 .
Central ductal portion filled with thrombotic material around coil loops in a lamb 107 days after stainless steel coil implantation. Methylmethacrylate embedded, ground section, toluidine blue stain. Black areas are the coil sections and the arrows indicate calcification. VW, vessel wall. × 200.
Figure 5 .
Central portion of an occluded ductus arteriosus in a lamb 32 days after nickel/titanium coil implantation, filled with fibromuscular cells. Methylmethacrylate embedded, ground section, toluidine blue stain. Black areas are the coil sections. × 200.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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