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. 1997 Feb;65(2):843–846. doi: 10.1128/iai.65.2.843-846.1997

Coccoid and spiral Helicobacter pylori differ in their abilities to adhere to gastric epithelial cells and induce interleukin-8 secretion.

S P Cole 1, D Cirillo 1, M F Kagnoff 1, D G Guiney 1, L Eckmann 1
PMCID: PMC176138  PMID: 9009355

Abstract

Helicobacter pylori exists as an actively dividing spiral form and a nonculturable, but viable, metabolizing coccoid form. Both forms are present in the stomach, but their relative pathophysiologic significances are unknown. Here we show that the coccoid form of H. pylori, in contrast to the spiral form, binds poorly to gastric epithelial cells and induces little, if any, interleukin-8 secretion by these cells.

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Selected References

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  1. Berry V., Jennings K., Woodnutt G. Bactericidal and morphological effects of amoxicillin on Helicobacter pylori. Antimicrob Agents Chemother. 1995 Aug;39(8):1859–1861. doi: 10.1128/aac.39.8.1859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blaser M. J., Parsonnet J. Parasitism by the "slow" bacterium Helicobacter pylori leads to altered gastric homeostasis and neoplasia. J Clin Invest. 1994 Jul;94(1):4–8. doi: 10.1172/JCI117336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bode G., Mauch F., Malfertheiner P. The coccoid forms of Helicobacter pylori. Criteria for their viability. Epidemiol Infect. 1993 Dec;111(3):483–490. doi: 10.1017/s0950268800057216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catrenich C. E., Makin K. M. Characterization of the morphologic conversion of Helicobacter pylori from bacillary to coccoid forms. Scand J Gastroenterol Suppl. 1991;181:58–64. [PubMed] [Google Scholar]
  5. Cellini L., Allocati N., Angelucci D., Iezzi T., Di Campli E., Marzio L., Dainelli B. Coccoid Helicobacter pylori not culturable in vitro reverts in mice. Microbiol Immunol. 1994;38(11):843–850. doi: 10.1111/j.1348-0421.1994.tb02136.x. [DOI] [PubMed] [Google Scholar]
  6. Cellini L., Allocati N., Di Campli E., Dainelli B. Helicobacter pylori: a fickle germ. Microbiol Immunol. 1994;38(1):25–30. doi: 10.1111/j.1348-0421.1994.tb01740.x. [DOI] [PubMed] [Google Scholar]
  7. Chan W. Y., Hui P. K., Leung K. M., Chow J., Kwok F., Ng C. S. Coccoid forms of Helicobacter pylori in the human stomach. Am J Clin Pathol. 1994 Oct;102(4):503–507. doi: 10.1093/ajcp/102.4.503. [DOI] [PubMed] [Google Scholar]
  8. Cover T. L., Tummuru M. K., Cao P., Thompson S. A., Blaser M. J. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J Biol Chem. 1994 Apr 8;269(14):10566–10573. [PubMed] [Google Scholar]
  9. Crabtree J. E., Wyatt J. I., Trejdosiewicz L. K., Peichl P., Nichols P. H., Ramsay N., Primrose J. N., Lindley I. J. Interleukin-8 expression in Helicobacter pylori infected, normal, and neoplastic gastroduodenal mucosa. J Clin Pathol. 1994 Jan;47(1):61–66. doi: 10.1136/jcp.47.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Eaton K. A., Catrenich C. E., Makin K. M., Krakowka S. Virulence of coccoid and bacillary forms of Helicobacter pylori in gnotobiotic piglets. J Infect Dis. 1995 Feb;171(2):459–462. doi: 10.1093/infdis/171.2.459. [DOI] [PubMed] [Google Scholar]
  11. Eckmann L., Jung H. C., Schürer-Maly C., Panja A., Morzycka-Wroblewska E., Kagnoff M. F. Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of interleukin 8. Gastroenterology. 1993 Dec;105(6):1689–1697. doi: 10.1016/0016-5085(93)91064-o. [DOI] [PubMed] [Google Scholar]
  12. Gribbon L. T., Barer M. R. Oxidative metabolism in nonculturable Helicobacter pylori and Vibrio vulnificus cells studied by substrate-enhanced tetrazolium reduction and digital image processing. Appl Environ Microbiol. 1995 Sep;61(9):3379–3384. doi: 10.1128/aem.61.9.3379-3384.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Huang J., O'Toole P. W., Doig P., Trust T. J. Stimulation of interleukin-8 production in epithelial cell lines by Helicobacter pylori. Infect Immun. 1995 May;63(5):1732–1738. doi: 10.1128/iai.63.5.1732-1738.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hulten K., Han S. W., Enroth H., Klein P. D., Opekun A. R., Gilman R. H., Evans D. G., Engstrand L., Graham D. Y., El-Zaatari F. A. Helicobacter pylori in the drinking water in Peru. Gastroenterology. 1996 Apr;110(4):1031–1035. doi: 10.1053/gast.1996.v110.pm8612990. [DOI] [PubMed] [Google Scholar]
  15. Janas B., Czkwianianc E., Bak-Romaniszyn L., Bartel H., Tosik D., Płaneta-Małecka I. Electron microscopic study of association between coccoid forms of Helicobacter pylori and gastric epithelial cells. Am J Gastroenterol. 1995 Oct;90(10):1829–1833. [PubMed] [Google Scholar]
  16. Noach L. A., Rolf T. M., Tytgat G. N. Electron microscopic study of association between Helicobacter pylori and gastric and duodenal mucosa. J Clin Pathol. 1994 Aug;47(8):699–704. doi: 10.1136/jcp.47.8.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reynolds D. J., Penn C. W. Characteristics of Helicobacter pylori growth in a defined medium and determination of its amino acid requirements. Microbiology. 1994 Oct;140(Pt 10):2649–2656. doi: 10.1099/00221287-140-10-2649. [DOI] [PubMed] [Google Scholar]
  18. Segal E. D., Falkow S., Tompkins L. S. Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins. Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1259–1264. doi: 10.1073/pnas.93.3.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shahamat M., Mai U., Paszko-Kolva C., Kessel M., Colwell R. R. Use of autoradiography to assess viability of Helicobacter pylori in water. Appl Environ Microbiol. 1993 Apr;59(4):1231–1235. doi: 10.1128/aem.59.4.1231-1235.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tummuru M. K., Cover T. L., Blaser M. J. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infect Immun. 1993 May;61(5):1799–1809. doi: 10.1128/iai.61.5.1799-1809.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tummuru M. K., Sharma S. A., Blaser M. J. Helicobacter pylori picB, a homologue of the Bordetella pertussis toxin secretion protein, is required for induction of IL-8 in gastric epithelial cells. Mol Microbiol. 1995 Dec;18(5):867–876. doi: 10.1111/j.1365-2958.1995.18050867.x. [DOI] [PubMed] [Google Scholar]

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