Abstract
The Staphylococcus aureus plasmid pSK1 carries Tn4001, a 4.7-kilobase (kb) transposon which specifies resistance to gentamicin, tobramycin, and kanamycin. In addition, pSK1 mediates resistance to trimethoprim and linked resistance to ethidium bromide (Ebr) and to quaternary ammonium compounds (Qar). Restriction endonuclease analysis of pSK1 and a deleted derivative of pSK1 revealed that the gene(s) responsible for Ebr Qar lies within a 5.2-kb HindIII fragment. This fragment has been cloned into the Escherichia coli plasmid vector pBR322, and transformants of an E. coli K-12 strain exhibited Ebr Qar. Subcloning of the 5.2-kb insert, combined with data from electron microscopic analysis of deleted derivatives of pSK1, located the Ebr Qar determinant(s) on a 2.3-kb segment of pSK1 DNA.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Archer G. L., Johnston J. L. Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides. Antimicrob Agents Chemother. 1983 Jul;24(1):70–77. doi: 10.1128/aac.24.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
- Hohn B. In vitro packaging of lambda and cosmid DNA. Methods Enzymol. 1979;68:299–309. doi: 10.1016/0076-6879(79)68021-7. [DOI] [PubMed] [Google Scholar]
- Horinouchi S., Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol. 1982 May;150(2):815–825. doi: 10.1128/jb.150.2.815-825.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iordanescu S., Surdeanu M. Two restriction and modification systems in Staphylococcus aureus NCTC8325. J Gen Microbiol. 1976 Oct;96(2):277–281. doi: 10.1099/00221287-96-2-277. [DOI] [PubMed] [Google Scholar]
- Jaffe H. W., Sweeney H. M., Weinstein R. A., Kabins S. A., Nathan C., Cohen S. Structural and phenotypic varieties of gentamicin resistance plasmids in hospital strains of Staphylococcus aureus and coagulase-negative staphylococci. Antimicrob Agents Chemother. 1982 May;21(5):773–779. doi: 10.1128/aac.21.5.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnston L. H., Dyke K. G. Ethidium bromide resistance, a new marker on the staphylococcal penicillinase plasmid. J Bacteriol. 1969 Dec;100(3):1413–1414. doi: 10.1128/jb.100.3.1413-1414.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lyon B. R., Iuorio J. L., May J. W., Skurray R. A. Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. J Med Microbiol. 1984 Feb;17(1):79–89. doi: 10.1099/00222615-17-1-79. [DOI] [PubMed] [Google Scholar]
- Lyon B. R., May J. W., Skurray R. A. Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1983 Jun;23(6):817–826. doi: 10.1128/aac.23.6.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lyon B. R., May J. W., Skurray R. A. Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Mol Gen Genet. 1984;193(3):554–556. doi: 10.1007/BF00382099. [DOI] [PubMed] [Google Scholar]
- McDonnell R. W., Sweeney H. M., Cohen S. Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother. 1983 Jan;23(1):151–160. doi: 10.1128/aac.23.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ray A., Skurray R. Cloning and polypeptide analysis of the leading region in F plasmid DNA transfer. Plasmid. 1983 May;9(3):262–272. doi: 10.1016/0147-619x(83)90004-5. [DOI] [PubMed] [Google Scholar]
- Townsend D. E., Greed L., Ashdown N., Grubb W. B. Plasmid-mediated resistance to quaternary ammonium compounds in methicillin-resistant Staphylococcus aureus. Med J Aust. 1983 Oct 1;2(7):310–310. [PubMed] [Google Scholar]
- Townsend D. E., Grubb W. B., Ashdown N. Gentamicin resistance in methicillin-resistant Staphylococcus aureus. Pathology. 1983 Apr;15(2):169–174. doi: 10.3109/00313028309084707. [DOI] [PubMed] [Google Scholar]