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. 1985 Feb;27(2):172–176. doi: 10.1128/aac.27.2.172

Involvement of oxidative damage in erythrocyte lysis induced by amphotericin B.

J Brajtburg, S Elberg, D R Schwartz, A Vertut-Croquin, D Schlessinger, G S Kobayashi, G Medoff
PMCID: PMC176232  PMID: 3985601

Abstract

Lysis of human erythrocytes induced by amphotericin B was retarded when the oxygen tension of the incubation mixture was reduced or when the antioxidant catalase was added; lysis was accelerated when cells were preincubated with the prooxidant ascorbate. In the atmosphere of reduced oxygen tension, the erythrocytes containing carboxyhemoglobin lysed at a slower rate than did the cells containing oxyhemoglobin. Consistent with a role for oxidative damage in lysis, the mixture of erythrocytes and amphotericin B showed an increase in malonyldialdehyde, the product of peroxidation, which paralleled the progression of hemolysis. In contrast, the permeabilizing effect of amphotericin B, measured as a decrease in intracellular K+, was not affected by changes in oxygen tension, catalase, or ascorbate treatment. These results imply that oxidant damage is involved in the lytic, but not in the permeabilizing, action of amphotericin B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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