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. 1985 Feb;27(2):172–176. doi: 10.1128/aac.27.2.172

Involvement of oxidative damage in erythrocyte lysis induced by amphotericin B.

J Brajtburg, S Elberg, D R Schwartz, A Vertut-Croquin, D Schlessinger, G S Kobayashi, G Medoff
PMCID: PMC176232  PMID: 3985601

Abstract

Lysis of human erythrocytes induced by amphotericin B was retarded when the oxygen tension of the incubation mixture was reduced or when the antioxidant catalase was added; lysis was accelerated when cells were preincubated with the prooxidant ascorbate. In the atmosphere of reduced oxygen tension, the erythrocytes containing carboxyhemoglobin lysed at a slower rate than did the cells containing oxyhemoglobin. Consistent with a role for oxidative damage in lysis, the mixture of erythrocytes and amphotericin B showed an increase in malonyldialdehyde, the product of peroxidation, which paralleled the progression of hemolysis. In contrast, the permeabilizing effect of amphotericin B, measured as a decrease in intracellular K+, was not affected by changes in oxygen tension, catalase, or ascorbate treatment. These results imply that oxidant damage is involved in the lytic, but not in the permeabilizing, action of amphotericin B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brajtburg J., Elberg S., Bolard J., Kobayashi G. S., Levy R. A., Ostlund R. E., Jr, Schlessinger D., Medoff G. Interaction of plasma proteins and lipoproteins with amphotericin B. J Infect Dis. 1984 Jun;149(6):986–997. doi: 10.1093/infdis/149.6.986. [DOI] [PubMed] [Google Scholar]
  2. Brajtburg J., Medoff G., Kobayashi G. S., Elberg S., Finegold C. Permeabilizing and hemolytic action of large and small polyene antibiotics on human erythrocytes. Antimicrob Agents Chemother. 1980 Oct;18(4):586–592. doi: 10.1128/aac.18.4.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen W. C., Chou D. L., Feingold D. S. Dissociation between ion permeability and the lethal action of polyene antibiotics on Candida albicans. Antimicrob Agents Chemother. 1978 Jun;13(6):914–917. doi: 10.1128/aac.13.6.914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cybulska B., Mazerski J., Borowski E., Gary-Bobo C. M. Haemolytic activity of aromatic heptaenes. A group of polyene macrolide antifungal antibiotics. Biochem Pharmacol. 1984 Jan 1;33(1):41–46. doi: 10.1016/0006-2952(84)90368-x. [DOI] [PubMed] [Google Scholar]
  5. Fridovich I. Superoxide radical: an endogenous toxicant. Annu Rev Pharmacol Toxicol. 1983;23:239–257. doi: 10.1146/annurev.pa.23.040183.001323. [DOI] [PubMed] [Google Scholar]
  6. Gutteridge J. M., Thomas A. H. Thiobarbituric acid reactivity of autoxidized candicidin. Biochem Med. 1980 Oct;24(2):194–200. doi: 10.1016/0006-2944(80)90012-5. [DOI] [PubMed] [Google Scholar]
  7. Hamada T., Furuya M., Hodate K. Protective effects of vitamin E and dithiothreitol against the hemolysis of rat and goat erythrocytes induced by Tween 20 with or without ascorbic acid and azide. Experientia. 1982 Apr 15;38(4):462–467. doi: 10.1007/BF01952638. [DOI] [PubMed] [Google Scholar]
  8. Medoff G., Brajtburg J., Kobayashi G. S., Bolard J. Antifungal agents useful in therapy of systemic fungal infections. Annu Rev Pharmacol Toxicol. 1983;23:303–330. doi: 10.1146/annurev.pa.23.040183.001511. [DOI] [PubMed] [Google Scholar]
  9. Mowri H., Nojima S., Inoue K. Effect of lipid composition of liposomes on their sensitivity to peroxidation. J Biochem. 1984 Feb;95(2):551–558. doi: 10.1093/oxfordjournals.jbchem.a134638. [DOI] [PubMed] [Google Scholar]
  10. Ryall R. G., Story C. J. An equilibrium model of the oxygen association curve of normal human erythrocytes under standardized conditions. Clin Chem. 1983 Oct;29(10):1819–1822. [PubMed] [Google Scholar]
  11. Sippel J. E., Levine H. B. Annulment of amphotericin B inhibition of Coccidioides immitis endospores. Effects on growth, respiration and morphogenesis. Sabouraudia. 1969 Oct;7(3):159–168. [PubMed] [Google Scholar]
  12. Trotta R. J., Sullivan S. G., Stern A. Lipid peroxidation and haemoglobin degradation in red blood cells exposed to t-butyl hydroperoxide. The relative roles of haem- and glutathione-dependent decomposition of t-butyl hydroperoxide and membrane lipid hydroperoxides in lipid peroxidation and haemolysis. Biochem J. 1983 Jun 15;212(3):759–772. doi: 10.1042/bj2120759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vladimirov Y. A., Olenev V. I., Suslova T. B., Cheremisina Z. P. Lipid peroxidation in mitochondrial membrane. Adv Lipid Res. 1980;17:173–249. doi: 10.1016/b978-0-12-024917-6.50011-2. [DOI] [PubMed] [Google Scholar]
  14. Weis M. R., Levine H. B. Inactivation of amphotericin B by reducing agents: influences on growth inhibition of Candida albicans and lysis of erythrocytes. Sabouraudia. 1972 Jul;10(2):132–142. doi: 10.1080/00362177285190271. [DOI] [PubMed] [Google Scholar]

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