Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1985 Nov;28(5):643–647. doi: 10.1128/aac.28.5.643

Effect of the imidazole derivative lombazole on the ultrastructure of Staphylococcus epidermidis and Candida albicans.

D Barug, K de Groot
PMCID: PMC176349  PMID: 3911878

Abstract

Lombazole, an antimicrobial agent of the imidazole class, induced profound ultrastructural changes in Staphylococcus epidermidis and Candida albicans, as observed by freeze fracture electron microscopy. In S. epidermidis cells, the primary effect on ultrastructure was characterized by a distinct change in the morphology of the plasma membrane. Secondary effects of lombazole were cell wall thickening, accumulations of lipidlike material, abnormal cell division, severe change of shape, separation of the plasma membrane from the cell wall, and disruption of cells. The alterations in C. albicans were characterized by the deformation of and a decrease in the number of invaginations in the protoplasmic fracture face and corresponding ridges on the exoplasmic fracture face and by separation of the plasma membrane from the cell wall, leaving a gap which frequently contained small vesicles. Moreover, a considerable thickening of the cell wall occurred at localized regions. These structural alterations are discussed in relation to biochemical changes which may correlate with these phenomena.

Full text

PDF
643

Images in this article

645Image
on p.645
646Image
on p.646

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barug D., Bastiaanse H. B. An evaluation of the antifungal effect of bifonazole on Torulopsis glabrata and Candida albicans under various in vitro test conditions. Arzneimittelforschung. 1983;33(4):524–528. [PubMed] [Google Scholar]
  2. Barug D., de Groot C. Microscopic studies of Candida albicans and Torulopsis glabrata after in vitro treatment with bifonazole. Freeze fracture electron microscopy. Arzneimittelforschung. 1983;33(4):538–545. [PubMed] [Google Scholar]
  3. De Nollin S., Borgers M. The ultrastructure of Candida albicans after in vitro treatment with miconazole. Sabouraudia. 1974 Nov;12(3):341–351. [PubMed] [Google Scholar]
  4. Giesbrecht P., Wecke J., Reinicke B. On the morphogenesis of the cell wall of staphylococci. Int Rev Cytol. 1976;44:225–318. doi: 10.1016/s0074-7696(08)61651-4. [DOI] [PubMed] [Google Scholar]
  5. Iwata K., Yamaguchi H., Hiratani T. Mode of action of clotrimazole. Sabouraudia. 1973 Jul;11(2):158–166. doi: 10.1080/00362177385190321. [DOI] [PubMed] [Google Scholar]
  6. Koike M., Iida K., Nakashima K., Tokunaga J. Small-cell formation of Staphylococcus aureus by subinhibitory concentrations of nitrofuran derivatives. J Bacteriol. 1974 Oct;120(1):524–526. doi: 10.1128/jb.120.1.524-526.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morgan C., Rosenkranz H. S., Carr H. S., Rose H. M. Electron microscopy of chloramphenicol-treated Escherichia coli. J Bacteriol. 1967 Jun;93(6):1987–2002. doi: 10.1128/jb.93.6.1987-2002.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Preusser H. J. Die Wirkung von Econazol auf die Feinstruktur der Zellen von Trichophyton rubrum. Mykosen. 1975 Nov;18(11):453–465. [PubMed] [Google Scholar]
  9. Preusser H. J., Rostek H. Freeze-fracture studies of the plasmalemma of Candida albicans after treatment with econazole-nitrate. Sabouraudia. 1979 Dec;17(4):389–398. [PubMed] [Google Scholar]
  10. Sargent M. G. Surface extension and the cell cycle in prokaryotes. Adv Microb Physiol. 1978;18:105–176. doi: 10.1016/s0065-2911(08)60416-6. [DOI] [PubMed] [Google Scholar]
  11. Smith P. F., Wilkinson B. J. Differential methicillin susceptibilities of peptidoglycan syntheses in methicillin-resistant Staphylococcus aureus. J Bacteriol. 1981 Nov;148(2):610–617. doi: 10.1128/jb.148.2.610-617.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Van Venetie R., Verkleij A. J. Analysis of the hexagonal II phase and its relations to lipidic particles and the lamellar phase. A freeze-fracture study. Biochim Biophys Acta. 1981 Jul 20;645(2):262–269. doi: 10.1016/0005-2736(81)90197-8. [DOI] [PubMed] [Google Scholar]
  13. Voigt W. H., Plempel M. Elektronenmikroskopische Untersuchungen an humapathogenen Pilzen. I. Ultrastrukturelle Veränderungen von Candida-albicans-Zellen durch Clotrimazol im Terexperiment. Arzneimittelforschung. 1974 Apr;24(4):508–515. [PubMed] [Google Scholar]
  14. Yamaguchi H., Hiratani T., Plempel M. In vitro studies of a new imidazole antimycotic, bifonazole, in comparison with clotrimazole and miconazole. Arzneimittelforschung. 1983;33(4):546–551. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES