Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1986 Feb;29(2):315–319. doi: 10.1128/aac.29.2.315

Plasmid-encoded amikacin resistance in multiresistant strains of Klebsiella pneumoniae isolated from neonates with meningitis.

M Woloj, M E Tolmasky, M C Roberts, J H Crosa
PMCID: PMC176398  PMID: 3521478

Abstract

Two multiresistant Klebsiella pneumoniae strains isolated from cerebrospinal fluid of human neonates were analyzed for their plasmid content. Two of the plasmids harbored by these strains, pJHCMW1 (11 kilobase pairs) and pJHCMW4 (75 kilobase pairs), carried genetic determinants for amikacin resistance. These plasmids also encoded resistance to kanamycin, tobramycin, and ampicillin which could be transferred to Escherichia coli by conjugation. Extracts from transconjugant derivatives carrying pJHCMW4 produced an acetyltransferase activity that acetylated all three aminoglycosides. Transconjugant derivatives carrying pJHCMW1 encoded both acetylating and phosphorylating activities. Southern blot hybridization analysis indicated considerable DNA homology between these two plasmids.

Full text

PDF
315

Images in this article

317Image
on p.317

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bongaerts G. P., Kaptijn G. M. Aminoglycoside phosphotransferase-II-mediated amikacin resistance in Escherichia coli. Antimicrob Agents Chemother. 1981 Sep;20(3):344–350. doi: 10.1128/aac.20.3.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  5. Cherubin C. E., Corrado M. L., Nair S. R., Gombert M. E., Landesman S., Humbert G. Treatment of gram-negative bacillary meningitis: role of the new cephalosporin antibiotics. Rev Infect Dis. 1982 Sep-Oct;4 (Suppl):S453–S464. doi: 10.1093/clinids/4.supplement_2.s453. [DOI] [PubMed] [Google Scholar]
  6. Coombe R. G., George A. M. New plasmid-mediated aminoglycoside adenylyltransferase of broad substrate range that adenylylates amikacin. Antimicrob Agents Chemother. 1981 Jul;20(1):75–80. doi: 10.1128/aac.20.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crosa J. H., Olarte J., Mata L. J., Luttropp L. K., Peñaranda M. E. Characterization of an R-plasmid associated with ampicillin resistance in Shigella dysenteriae type 1 isolated from epidemics. Antimicrob Agents Chemother. 1977 Mar;11(3):553–558. doi: 10.1128/aac.11.3.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crosa J. H., Schiewe M. H., Falkow S. Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum. Infect Immun. 1977 Nov;18(2):509–513. doi: 10.1128/iai.18.2.509-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Datta N., Hughes V. M., Nugent M. E., Richards H. Plasmids and transposons and their stability and mutability in bacteria isolated during an outbreak of hospital infection. Plasmid. 1979 Apr;2(2):182–196. doi: 10.1016/0147-619x(79)90037-4. [DOI] [PubMed] [Google Scholar]
  10. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  11. Guiney D. G., Helinski D. R. The DNA-protein relaxation complex of the plasmid RK2: location of the site-specific nick in the region of the proposed origin of transfer. Mol Gen Genet. 1979 Oct 3;176(2):183–189. doi: 10.1007/BF00273212. [DOI] [PubMed] [Google Scholar]
  12. Haas M. J., Dowding J. E. Aminoglycoside-modifying enzymes. Methods Enzymol. 1975;43:611–628. doi: 10.1016/0076-6879(75)43124-x. [DOI] [PubMed] [Google Scholar]
  13. John J. F., Jr, McNeill W. F., Price K. E., Kresel P. A. Evidence for a chromosomal site specifying amikacin resistance in multiresistant Serratia marcescens. Antimicrob Agents Chemother. 1982 Apr;21(4):587–591. doi: 10.1128/aac.21.4.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Levine J. F., Maslow M. J., Leibowitz R. E., Pollock A. A., Hanna B. A., Schaefler S., Simberkoff M. S., Rahal J. J., Jr Amikacin-resistant gram-negative bacilli: correlation of occurrence with amikacin use. J Infect Dis. 1985 Feb;151(2):295–300. doi: 10.1093/infdis/151.2.295. [DOI] [PubMed] [Google Scholar]
  16. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. doi: 10.1016/0147-619x(78)90056-2. [DOI] [PubMed] [Google Scholar]
  17. McConaughy B. L., Laird C. D., McCarthy B. J. Nucleic acid reassociation in formamide. Biochemistry. 1969 Aug;8(8):3289–3295. doi: 10.1021/bi00836a024. [DOI] [PubMed] [Google Scholar]
  18. Meyer J. F., Nies B. A., Wiedemann B. Amikacin resistance mediated by multiresistance transposon Tn2424. J Bacteriol. 1983 Aug;155(2):755–760. doi: 10.1128/jb.155.2.755-760.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Murray B. E., Moellering R. C., Jr In-vivo acquisition of two different types of aminoglycoside resistance by a single strain of Klebsiella pneumoniae causing severe infection. Ann Intern Med. 1982 Feb;96(2):176–180. doi: 10.7326/0003-4819-96-2-176. [DOI] [PubMed] [Google Scholar]
  20. O'Brien T. F., Ross D. G., Guzman M. A., Medeiros A. A., Hedges R. W., Botstein D. Dissemination of an antibiotic resistance plasmid in hospital patient flora. Antimicrob Agents Chemother. 1980 Apr;17(4):537–543. doi: 10.1128/aac.17.4.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Perlin M. H., Lerner S. A. Amikacin resistance associated with a plasmid-borne aminoglycoside phosphotransferase in Escherichia coli. Antimicrob Agents Chemother. 1979 Nov;16(5):598–604. doi: 10.1128/aac.16.5.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Radika K., Northrop D. B. Correlation of antibiotic resistance with Vmax/Km ratio of enzymatic modification of aminoglycosides by kanamycin acetyltransferase. Antimicrob Agents Chemother. 1984 Apr;25(4):479–482. doi: 10.1128/aac.25.4.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  24. Rubens C. E., McNeill W. F., Farrar W. E., Jr Evolution of multiple-antibiotic-resistance plasmids mediated by transposable plasmid deoxyribonucleic acid sequences. J Bacteriol. 1979 Nov;140(2):713–719. doi: 10.1128/jb.140.2.713-719.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sadowski P. L., Peterson B. C., Gerding D. N., Cleary P. P. Physical characterization of ten R plasmids obtained from an outbreak of nosocomial Klebsiella pneumoniae infections. Antimicrob Agents Chemother. 1979 Apr;15(4):616–624. doi: 10.1128/aac.15.4.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith A. L., Smith D. H. Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin. J Infect Dis. 1974 Apr;129(4):391–401. doi: 10.1093/infdis/129.4.391. [DOI] [PubMed] [Google Scholar]
  27. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  28. Tolmasky M. E., Crosa J. H. Molecular cloning and expression of genetic determinants for the iron uptake system mediated by the Vibrio anguillarum plasmid pJM1. J Bacteriol. 1984 Dec;160(3):860–866. doi: 10.1128/jb.160.3.860-866.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Walter M. A., Potter S. A., Crosa J. H. Iron uptake system medicated by Vibrio anguillarum plasmid pJM1. J Bacteriol. 1983 Nov;156(2):880–887. doi: 10.1128/jb.156.2.880-887.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES