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. 1986 Oct;30(4):570–573. doi: 10.1128/aac.30.4.570

Comparative methods for detection of thymidine kinase-deficient herpes simplex virus type 1 strains.

J Harmenberg, V A Sundqvist, H Gadler, B Levén, G Brännström, B Wahren
PMCID: PMC176482  PMID: 3024561

Abstract

Four methods for analyzing viral susceptibility to antiviral substances were compared. In two methods viral products were measured: late viral proteins were measured by an enzyme-linked immunosorbent assay and viral DNA was measured by DNA hybridization. Infectious virus was quantified in the other two assays as the number of plaques and the yield of virus. The enzyme-linked immunosorbent assay procedure in our hands detected the smallest amounts (lowest proportions) of thymidine kinase-deficient herpes simplex virus type 1 mixed with wild-type virus. The thymidine kinase-deficient proportion of the herpes simplex virus type 1 isolate increased rapidly in the presence of acyclovir in cell culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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