Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Jan;177(1):238–241. doi: 10.1128/jb.177.1.238-241.1995

The TyrR protein of Escherichia coli is a class I transcription activator.

B Lawley 1, N Fujita 1, A Ishihama 1, A J Pittard 1
PMCID: PMC176579  PMID: 7798138

Abstract

The purified TyrR protein and phenylalanine were sufficient to activate in vitro transcription from the tyrP promoter by wild-type RNA polymerase. Such TyrR-mediated activation did not occur when the mutant alpha 235 RNA polymerase was used, indicating that TyrR is a class I transcription activator.

Full Text

The Full Text of this article is available as a PDF (469.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews A. E., Dickson B., Lawley B., Cobbett C., Pittard A. J. Importance of the position of TYR R boxes for repression and activation of the tyrP and aroF genes in Escherichia coli. J Bacteriol. 1991 Aug;173(16):5079–5085. doi: 10.1128/jb.173.16.5079-5085.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andrews A. E., Lawley B., Pittard A. J. Mutational analysis of repression and activation of the tyrP gene in Escherichia coli. J Bacteriol. 1991 Aug;173(16):5068–5078. doi: 10.1128/jb.173.16.5068-5078.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ho Y. S., Wulff D. L., Rosenberg M. Bacteriophage lambda protein cII binds promoters on the opposite face of the DNA helix from RNA polymerase. Nature. 1983 Aug 25;304(5928):703–708. doi: 10.1038/304703a0. [DOI] [PubMed] [Google Scholar]
  4. Igarashi K., Hanamura A., Makino K., Aiba H., Aiba H., Mizuno T., Nakata A., Ishihama A. Functional map of the alpha subunit of Escherichia coli RNA polymerase: two modes of transcription activation by positive factors. Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8958–8962. doi: 10.1073/pnas.88.20.8958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Igarashi K., Ishihama A. Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP. Cell. 1991 Jun 14;65(6):1015–1022. doi: 10.1016/0092-8674(91)90553-b. [DOI] [PubMed] [Google Scholar]
  6. Ishihama A. Protein-protein communication within the transcription apparatus. J Bacteriol. 1993 May;175(9):2483–2489. doi: 10.1128/jb.175.9.2483-2489.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kasian P. A., Davidson B. E., Pittard J. Molecular analysis of the promoter operator region of the Escherichia coli K-12 tyrP gene. J Bacteriol. 1986 Aug;167(2):556–561. doi: 10.1128/jb.167.2.556-561.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kolb A., Igarashi K., Ishihama A., Lavigne M., Buckle M., Buc H. E. coli RNA polymerase, deleted in the C-terminal part of its alpha-subunit, interacts differently with the cAMP-CRP complex at the lacP1 and at the galP1 promoter. Nucleic Acids Res. 1993 Jan 25;21(2):319–326. doi: 10.1093/nar/21.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kumar A., Grimes B., Fujita N., Makino K., Malloch R. A., Hayward R. S., Ishihama A. Role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation. J Mol Biol. 1994 Jan 14;235(2):405–413. doi: 10.1006/jmbi.1994.1001. [DOI] [PubMed] [Google Scholar]
  10. Lee H. S., Ishihama A., Kustu S. The C terminus of the alpha subunit of RNA polymerase is not essential for transcriptional activation of sigma 54 holoenzyme. J Bacteriol. 1993 Apr;175(8):2479–2482. doi: 10.1128/jb.175.8.2479-2482.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Li M., Moyle H., Susskind M. M. Target of the transcriptional activation function of phage lambda cI protein. Science. 1994 Jan 7;263(5143):75–77. doi: 10.1126/science.8272867. [DOI] [PubMed] [Google Scholar]
  12. Maeda S., Ozawa Y., Mizuno T., Mizushima S. Stereospecific positioning of the cis-acting sequence with respect to the canonical promoter is required for activation of the ompC gene by a positive regulator, OmpR, in Escherichia coli. J Mol Biol. 1988 Aug 5;202(3):433–441. doi: 10.1016/0022-2836(88)90276-8. [DOI] [PubMed] [Google Scholar]
  13. Makino K., Amemura M., Kim S. K., Nakata A., Shinagawa H. Role of the sigma 70 subunit of RNA polymerase in transcriptional activation by activator protein PhoB in Escherichia coli. Genes Dev. 1993 Jan;7(1):149–160. doi: 10.1101/gad.7.1.149. [DOI] [PubMed] [Google Scholar]
  14. Makino K., Shinagawa H., Amemura M., Kimura S., Nakata A., Ishihama A. Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro. J Mol Biol. 1988 Sep 5;203(1):85–95. doi: 10.1016/0022-2836(88)90093-9. [DOI] [PubMed] [Google Scholar]
  15. Menendez M., Kolb A., Buc H. A new target for CRP action at the malT promoter. EMBO J. 1987 Dec 20;6(13):4227–4234. doi: 10.1002/j.1460-2075.1987.tb02771.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Menon K. P., Lee N. L. Activation of ara operons by a truncated AraC protein does not require inducer. Proc Natl Acad Sci U S A. 1990 May;87(10):3708–3712. doi: 10.1073/pnas.87.10.3708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sakumi K., Igarashi K., Sekiguchi M., Ishihama A. The Ada protein is a class I transcription factor of Escherichia coli. J Bacteriol. 1993 Apr;175(8):2455–2457. doi: 10.1128/jb.175.8.2455-2457.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stock J. B., Ninfa A. J., Stock A. M. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev. 1989 Dec;53(4):450–490. doi: 10.1128/mr.53.4.450-490.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tao K., Fujita N., Ishihama A. Involvement of the RNA polymerase alpha subunit C-terminal region in co-operative interaction and transcriptional activation with OxyR protein. Mol Microbiol. 1993 Mar;7(6):859–864. doi: 10.1111/j.1365-2958.1993.tb01176.x. [DOI] [PubMed] [Google Scholar]
  20. Whipp M. J., Pittard A. J. Regulation of aromatic amino acid transport systems in Escherichia coli K-12. J Bacteriol. 1977 Nov;132(2):453–461. doi: 10.1128/jb.132.2.453-461.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yang J., Camakaris H., Pittard A. J. Mutations in the tyrR gene of Escherichia coli which affect TyrR-mediated activation but not TyrR-mediated repression. J Bacteriol. 1993 Oct;175(19):6372–6375. doi: 10.1128/jb.175.19.6372-6375.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yang J., Ganesan S., Sarsero J., Pittard A. J. A genetic analysis of various functions of the TyrR protein of Escherichia coli. J Bacteriol. 1993 Mar;175(6):1767–1776. doi: 10.1128/jb.175.6.1767-1776.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES