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. 1995 Jan;177(1):247–251. doi: 10.1128/jb.177.1.247-251.1995

Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.

J Ras 1, P W Van Ophem 1, W N Reijnders 1, R J Van Spanning 1, J A Duine 1, A H Stouthamer 1, N Harms 1
PMCID: PMC176581  PMID: 7798140

Abstract

NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.

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Selected References

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