Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Jan;177(2):473–476. doi: 10.1128/jb.177.2.473-476.1995

Mapping of genes involved in macromolecular synthesis on the chromosome of Streptomyces coelicolor A3(2).

G P van Wezel 1, M J Buttner 1, E Vijgenboom 1, L Bosch 1, D A Hopwood 1, H M Kieser 1
PMCID: PMC176614  PMID: 7814340

Abstract

The genes for the beta, beta', and seven sigma factor subunits of RNA polymerase, for elongation factors EF-Tu1 and EF-Tu3, and for six rRNA operons were mapped on the combined genetic and physical map of the Streptomyces coelicolor chromosome. Like the previously mapped tRNA genes, the RNA polymerase and rRNA genes map to scattered positions. The lack of rRNA operons in the immediate vicinity of the origin of replication (oriC) and the absence of tRNA genes in any of the rRNA operons are novel features of the Streptomyces chromosome.

Full Text

The Full Text of this article is available as a PDF (204.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aboshkiwa M., al-Ani B., Coleman G., Rowland G. Cloning and physical mapping of the Staphylococcus aureus rplL, rpoB and rpoC genes, encoding ribosomal protein L7/L12 and RNA polymerase subunits beta and beta'. J Gen Microbiol. 1992 Sep;138(9):1875–1880. doi: 10.1099/00221287-138-9-1875. [DOI] [PubMed] [Google Scholar]
  2. An G., Friesen J. D. The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. Gene. 1980 Dec;12(1-2):33–39. doi: 10.1016/0378-1119(80)90013-x. [DOI] [PubMed] [Google Scholar]
  3. Baylis H. A., Bibb M. J. Organisation of the ribosomal RNA genes in Streptomyces coelicolor A3(2). Mol Gen Genet. 1988 Feb;211(2):191–196. doi: 10.1007/BF00330593. [DOI] [PubMed] [Google Scholar]
  4. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol. 1981 May 15;148(2):107–127. doi: 10.1016/0022-2836(81)90508-8. [DOI] [PubMed] [Google Scholar]
  5. Brown K. L., Wood S., Buttner M. J. Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3(2); renaturation of a sigma subunit using GroEL. Mol Microbiol. 1992 May;6(9):1133–1139. doi: 10.1111/j.1365-2958.1992.tb01551.x. [DOI] [PubMed] [Google Scholar]
  6. Buttner M. J., Chater K. F., Bibb M. J. Cloning, disruption, and transcriptional analysis of three RNA polymerase sigma factor genes of Streptomyces coelicolor A3(2). J Bacteriol. 1990 Jun;172(6):3367–3378. doi: 10.1128/jb.172.6.3367-3378.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Buttner M. J., Lewis C. G. Construction and characterization of Streptomyces coelicolor A3(2) mutants that are multiply deficient in the nonessential hrd-encoded RNA polymerase sigma factors. J Bacteriol. 1992 Aug;174(15):5165–5167. doi: 10.1128/jb.174.15.5165-5167.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Buttner M. J., Smith A. M., Bibb M. J. At least three different RNA polymerase holoenzymes direct transcription of the agarase gene (dagA) of Streptomyces coelicolor A3(2). Cell. 1988 Feb 26;52(4):599–607. doi: 10.1016/0092-8674(88)90472-2. [DOI] [PubMed] [Google Scholar]
  9. Calcutt M. J., Schmidt F. J. Conserved gene arrangement in the origin region of the Streptomyces coelicolor chromosome. J Bacteriol. 1992 May;174(10):3220–3226. doi: 10.1128/jb.174.10.3220-3226.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Casjens S., Huang W. M. Linear chromosomal physical and genetic map of Borrelia burgdorferi, the Lyme disease agent. Mol Microbiol. 1993 May;8(5):967–980. doi: 10.1111/j.1365-2958.1993.tb01641.x. [DOI] [PubMed] [Google Scholar]
  11. Chater K. F., Bruton C. J., Plaskitt K. A., Buttner M. J., Méndez C., Helmann J. D. The developmental fate of S. coelicolor hyphae depends upon a gene product homologous with the motility sigma factor of B. subtilis. Cell. 1989 Oct 6;59(1):133–143. doi: 10.1016/0092-8674(89)90876-3. [DOI] [PubMed] [Google Scholar]
  12. Chater K. F. Rifampicin-resistant mutants of Streptomyces coelicolor A3(2). J Gen Microbiol. 1974 Jan;80(1):277–290. doi: 10.1099/00221287-80-1-277. [DOI] [PubMed] [Google Scholar]
  13. Flett F., Platt J., Oliver S. G. Isolation and characterization of temperature-sensitive mutants of Streptomyces coelicolor A3(2) blocked in macromolecular synthesis. J Gen Microbiol. 1992 Mar;138(3):579–585. doi: 10.1099/00221287-138-3-579. [DOI] [PubMed] [Google Scholar]
  14. Forchhammer K., Leinfelder W., Böck A. Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein. Nature. 1989 Nov 23;342(6248):453–456. doi: 10.1038/342453a0. [DOI] [PubMed] [Google Scholar]
  15. Honoré N., Bergh S., Chanteau S., Doucet-Populaire F., Eiglmeier K., Garnier T., Georges C., Launois P., Limpaiboon T., Newton S. Nucleotide sequence of the first cosmid from the Mycobacterium leprae genome project: structure and function of the Rif-Str regions. Mol Microbiol. 1993 Jan;7(2):207–214. doi: 10.1111/j.1365-2958.1993.tb01112.x. [DOI] [PubMed] [Google Scholar]
  16. Hopwood D. A. Nonrandom Location of Temperature-Sensitive Mutants on the Linkage Map of STREPTOMYCES COELICOLOR. Genetics. 1966 Nov;54(5):1169–1176. doi: 10.1093/genetics/54.5.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kieser H. M., Kieser T., Hopwood D. A. A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome. J Bacteriol. 1992 Sep;174(17):5496–5507. doi: 10.1128/jb.174.17.5496-5507.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  19. Lawlor E. J., Baylis H. A., Chater K. F. Pleiotropic morphological and antibiotic deficiencies result from mutations in a gene encoding a tRNA-like product in Streptomyces coelicolor A3(2). Genes Dev. 1987 Dec;1(10):1305–1310. doi: 10.1101/gad.1.10.1305. [DOI] [PubMed] [Google Scholar]
  20. Leblond P., Redenbach M., Cullum J. Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2). J Bacteriol. 1993 Jun;175(11):3422–3429. doi: 10.1128/jb.175.11.3422-3429.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lin Y. S., Kieser H. M., Hopwood D. A., Chen C. W. The chromosomal DNA of Streptomyces lividans 66 is linear. Mol Microbiol. 1993 Dec;10(5):923–933. doi: 10.1111/j.1365-2958.1993.tb00964.x. [DOI] [PubMed] [Google Scholar]
  22. Lonetto M. A., Brown K. L., Rudd K. E., Buttner M. J. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7573–7577. doi: 10.1073/pnas.91.16.7573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lonetto M., Gribskov M., Gross C. A. The sigma 70 family: sequence conservation and evolutionary relationships. J Bacteriol. 1992 Jun;174(12):3843–3849. doi: 10.1128/jb.174.12.3843-3849.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mendez C., Chater K. F. Cloning of whiG, a gene critical for sporulation of Streptomyces coelicolor A3(2). J Bacteriol. 1987 Dec;169(12):5715–5720. doi: 10.1128/jb.169.12.5715-5720.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Musialowski M. S., Flett F., Scott G. B., Hobbs G., Smith C. P., Oliver S. G. Functional evidence that the principal DNA replication origin of the Streptomyces coelicolor chromosome is close to the dnaA-gyrB region. J Bacteriol. 1994 Aug;176(16):5123–5125. doi: 10.1128/jb.176.16.5123-5125.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Médigue C., Viari A., Hénaut A., Danchin A. Escherichia coli molecular genetic map (1500 kbp): update II. Mol Microbiol. 1991 Nov;5(11):2629–2640. doi: 10.1111/j.1365-2958.1991.tb01972.x. [DOI] [PubMed] [Google Scholar]
  27. Redenbach M., Flett F., Piendl W., Glocker I., Rauland U., Wafzig O., Kliem R., Leblond P., Cullum J. The Streptomyces lividans 66 chromosome contains a 1 MB deletogenic region flanked by two amplifiable regions. Mol Gen Genet. 1993 Nov;241(3-4):255–262. doi: 10.1007/BF00284676. [DOI] [PubMed] [Google Scholar]
  28. Sedlmeier R., Werner T., Kieser H. M., Hopwood D. A., Schmieger H. tRNA genes of Streptomyces lividans: new sequences and comparison of structure and organization with those of other bacteria. J Bacteriol. 1994 Sep;176(17):5550–5553. doi: 10.1128/jb.176.17.5550-5553.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shiina T., Tanaka K., Takahashi H. Sequence of hrdB, an essential gene encoding sigma-like transcription factor of Streptomyces coelicolor A3(2): homology to principal sigma factors. Gene. 1991 Oct 30;107(1):145–148. doi: 10.1016/0378-1119(91)90308-x. [DOI] [PubMed] [Google Scholar]
  30. Tanaka K., Shiina T., Takahashi H. Multiple principal sigma factor homologs in eubacteria: identification of the "rpoD box". Science. 1988 Nov 18;242(4881):1040–1042. doi: 10.1126/science.3194753. [DOI] [PubMed] [Google Scholar]
  31. Tanaka K., Shiina T., Takahashi H. Nucleotide sequence of genes hrdA, hrdC, and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes. Mol Gen Genet. 1991 Oct;229(3):334–340. doi: 10.1007/BF00267453. [DOI] [PubMed] [Google Scholar]
  32. Versalovic J., Koeuth T., Britton R., Geszvain K., Lupski J. R. Conservation and evolution of the rpsU-dnaG-rpoD macromolecular synthesis operon in bacteria. Mol Microbiol. 1993 Apr;8(2):343–355. doi: 10.1111/j.1365-2958.1993.tb01578.x. [DOI] [PubMed] [Google Scholar]
  33. Vijgenboom E., Woudt L. P., Heinstra P. W., Rietveld K., van Haarlem J., van Wezel G. P., Shochat S., Bosch L. Three tuf-like genes in the kirromycin producer Streptomyces ramocissimus. Microbiology. 1994 Apr;140(Pt 4):983–998. doi: 10.1099/00221287-140-4-983. [DOI] [PubMed] [Google Scholar]
  34. Xie W. Q., Jäger K., Potts M. Cyanobacterial RNA polymerase genes rpoC1 and rpoC2 correspond to rpoC of Escherichia coli. J Bacteriol. 1989 Apr;171(4):1967–1973. doi: 10.1128/jb.171.4.1967-1973.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Yokota T., Sugisaki H., Takanami M., Kaziro Y. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Gene. 1980 Dec;12(1-2):25–31. doi: 10.1016/0378-1119(80)90012-8. [DOI] [PubMed] [Google Scholar]
  36. Zakrzewska-Czerwińska J., Schrempf H. Characterization of an autonomously replicating region from the Streptomyces lividans chromosome. J Bacteriol. 1992 Apr;174(8):2688–2693. doi: 10.1128/jb.174.8.2688-2693.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. van Wezel G. P., Vijgenboom E., Bosch L. A comparative study of the ribosomal RNA operons of Streptomyces coelicolor A3(2) and sequence analysis of rrnA. Nucleic Acids Res. 1991 Aug 25;19(16):4399–4403. doi: 10.1093/nar/19.16.4399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. van Wezel G. P., Woudt L. P., Vervenne R., Verdurmen M. L., Vijgenboom E., Bosch L. Cloning and sequencing of the tuf genes of Streptomyces coelicolor A3(2). Biochim Biophys Acta. 1994 Oct 18;1219(2):543–547. doi: 10.1016/0167-4781(94)90085-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES