CELL BIOLOGY. For the article “MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myogenic cells,” by Joan C. Ritland Politz Fan Zhang and Thoru Pederson, which appeared in issue 50, December 12, 2006, of Proc Natl Acad Sci USA (103:18957–18962; first published November 29, 2006; 10.1073/pnas.0609466103), the authors note that in Fig. 5, A and C were transposed. The corrected figure and its legend appear below. This error does not affect the conclusions of the article.
Fig. 5.
miR-206 is not concentrated in nucleolar FCs but is concentrated in the GC. L6 myoblasts were subjected to immunostaining for UBF (to mark FCs, A–H) or nucleostemin (to mark the GC, I–P) followed by in situ hybridization to miR-206, and image stacks were captured and subjected to 3D deconvolution. Enlarged images of deconvolved nucleoli show miR-206 (A and D) and corresponding UBF (C and F) distribution, and pseudocolored images (B and E) show miR-206 (red) and UBF (green) overlap (yellow). Linescan in G corresponds to the line in B, and linescan in H corresponds to the line in E. Similarly, enlarged images of deconvolved nucleoli show miR-206 (I and L) and corresponding nucleostemin (K and N) distribution, and pseudocolored images (J and M) show miR-206 (red) and nucleostemin (green) overlap (yellow). Linescan in O corresponds to the line in J, and linescan in P corresponds to the line in N. Images are 9.9 μm wide (A–C), 5.9 μm wide (D–F), 7 μm wide (I–K), and 7.3 μm wide (L–N).