Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Feb;177(3):843–846. doi: 10.1128/jb.177.3.843-846.1995

A spontaneous hemadsorption-negative mutant of Mycoplasma pneumoniae exhibits a truncated adhesin-related 30-kilodalton protein and lacks the cytadherence-accessory protein HMW1.

G Layh-Schmitt 1, H Hilbert 1, E Pirkl 1
PMCID: PMC176669  PMID: 7836325

Abstract

A spontaneous, hemadsorption-negative mutant of Mycoplasma pneumoniae lacks the cytoskeleton-forming HMW1 protein and exhibits a truncated adhesin-related 30-kDa protein. Genetic analyses revealed deletion of one nucleotide in the hmw1 gene and loss of eight repeated sequences comprising 144 nucleotides in the gene for the adhesin-related 30-kDa protein.

Full Text

The Full Text of this article is available as a PDF (229.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albertini A. M., Hofer M., Calos M. P., Miller J. H. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions. Cell. 1982 Jun;29(2):319–328. doi: 10.1016/0092-8674(82)90148-9. [DOI] [PubMed] [Google Scholar]
  2. Barile M. F., Chandler D. K., Yoshida H., Grabowski M. W., Harasawa R., Razin S. Parameters of Mycoplasma pneumoniae infection in Syrian hamsters. Infect Immun. 1988 Sep;56(9):2443–2449. doi: 10.1128/iai.56.9.2443-2449.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baseman J. B., Cole R. M., Krause D. C., Leith D. K. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol. 1982 Sep;151(3):1514–1522. doi: 10.1128/jb.151.3.1514-1522.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baseman J. B., Morrison-Plummer J., Drouillard D., Puleo-Scheppke B., Tryon V. V., Holt S. C. Identification of a 32-kilodalton protein of Mycoplasma pneumoniae associated with hemadsorption. Isr J Med Sci. 1987 May;23(5):474–479. [PubMed] [Google Scholar]
  5. Boxer L. M., Korn D. Structural and enzymological characterization of the homogeneous deoxyribonucleic acid polymerase from Mycoplasma orale. Biochemistry. 1979 Oct 16;18(21):4742–4749. [PubMed] [Google Scholar]
  6. Dallo S. F., Chavoya A., Baseman J. B. Characterization of the gene for a 30-kilodalton adhesion-related protein of Mycoplasma pneumoniae. Infect Immun. 1990 Dec;58(12):4163–4165. doi: 10.1128/iai.58.12.4163-4165.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feldner J., Bredt W. Analysis of polypeptides of mutants of Mycoplasma pneumoniae that lack the ability to haemadsorb. J Gen Microbiol. 1983 Mar;129(3):841–848. doi: 10.1099/00221287-129-3-841. [DOI] [PubMed] [Google Scholar]
  8. Feldner J., Göbel U., Bredt W. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature. 1982 Aug 19;298(5876):765–767. doi: 10.1038/298765a0. [DOI] [PubMed] [Google Scholar]
  9. Franzoso G., Hu P. C., Meloni G. A., Barile M. F. The immunodominant 90-kilodalton protein is localized on the terminal tip structure of Mycoplasma pneumoniae. Infect Immun. 1993 Apr;61(4):1523–1530. doi: 10.1128/iai.61.4.1523-1530.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hansen E. J., Wilson R. M., Baseman J. B. Isolation of mutants of Mycoplasma pneumoniae defective in hemadsorption. Infect Immun. 1979 Mar;23(3):903–906. doi: 10.1128/iai.23.3.903-906.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hansen E. J., Wilson R. M., Clyde W. A., Jr, Baseman J. B. Characterization of hemadsorption-negative mutants of Mycoplasma pneumoniae. Infect Immun. 1981 Apr;32(1):127–136. doi: 10.1128/iai.32.1.127-136.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  13. Hu P. C., Cole R. M., Huang Y. S., Graham J. A., Gardner D. E., Collier A. M., Clyde W. A., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. doi: 10.1126/science.6801766. [DOI] [PubMed] [Google Scholar]
  14. Hu P. C., Collier A. M., Baseman J. B. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J Exp Med. 1977 May 1;145(5):1328–1343. doi: 10.1084/jem.145.5.1328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hu P. C., Collier A. M., Clyde W. A., Jr Serological comparison of virulent and avirulent Mycoplasma pneumoniae by monoclonal antibodies. Isr J Med Sci. 1984 Sep;20(9):870–873. [PubMed] [Google Scholar]
  16. Inamine J. M., Denny T. P., Loechel S., Schaper U., Huang C. H., Bott K. F., Hu P. C. Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniae. Gene. 1988 Apr 29;64(2):217–229. doi: 10.1016/0378-1119(88)90337-x. [DOI] [PubMed] [Google Scholar]
  17. Inamine J. M., Loechel S., Hu P. C. Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae. Gene. 1988 Dec 15;73(1):175–183. doi: 10.1016/0378-1119(88)90323-x. [DOI] [PubMed] [Google Scholar]
  18. Krause D. C., Lee K. K. Juxtaposition of the genes encoding Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW3. Gene. 1991 Oct 30;107(1):83–89. doi: 10.1016/0378-1119(91)90300-z. [DOI] [PubMed] [Google Scholar]
  19. Krause D. C., Leith D. K., Baseman J. B. Reacquisition of specific proteins confers virulence in Mycoplasma pneumoniae. Infect Immun. 1983 Feb;39(2):830–836. doi: 10.1128/iai.39.2.830-836.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Krause D. C., Leith D. K., Wilson R. M., Baseman J. B. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect Immun. 1982 Mar;35(3):809–817. doi: 10.1128/iai.35.3.809-817.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  22. Layh-Schmitt G., Herrmann R. Localization and biochemical characterization of the ORF6 gene product of the Mycoplasma pneumoniae P1 operon. Infect Immun. 1992 Jul;60(7):2906–2913. doi: 10.1128/iai.60.7.2906-2913.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Layh-Schmitt G., Herrmann R. Spatial arrangement of gene products of the P1 operon in the membrane of Mycoplasma pneumoniae. Infect Immun. 1994 Mar;62(3):974–979. doi: 10.1128/iai.62.3.974-979.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Layh-Schmitt G. The ORF6 gene product of the P1 operon of Mycoplasma pneumoniae. Zentralbl Bakteriol. 1993 Apr;278(2-3):287–295. doi: 10.1016/s0934-8840(11)80845-5. [DOI] [PubMed] [Google Scholar]
  25. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  26. Needleman S. B., Wunsch C. D. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol. 1970 Mar;48(3):443–453. doi: 10.1016/0022-2836(70)90057-4. [DOI] [PubMed] [Google Scholar]
  27. Ogle K. F., Lee K. K., Krause D. C. Nucleotide sequence analysis reveals novel features of the phase-variable cytadherence accessory protein HMW3 of Mycoplasma pneumoniae. Infect Immun. 1992 Apr;60(4):1633–1641. doi: 10.1128/iai.60.4.1633-1641.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Proft T., Herrmann R. Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins. Mol Microbiol. 1994 Jul;13(2):337–348. doi: 10.1111/j.1365-2958.1994.tb00427.x. [DOI] [PubMed] [Google Scholar]
  29. Razin S. The mycoplasmas. Microbiol Rev. 1978 Jun;42(2):414–470. doi: 10.1128/mr.42.2.414-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sperker B., Hu P., Herrmann R. Identification of gene products of the P1 operon of Mycoplasma pneumoniae. Mol Microbiol. 1991 Feb;5(2):299–306. doi: 10.1111/j.1365-2958.1991.tb02110.x. [DOI] [PubMed] [Google Scholar]
  32. Stevens M. K., Krause D. C. Disulfide-linked protein associated with Mycoplasma pneumoniae cytadherence phase variation. Infect Immun. 1990 Oct;58(10):3430–3433. doi: 10.1128/iai.58.10.3430-3433.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Stevens M. K., Krause D. C. Localization of the Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW4 in the cytoskeletonlike Triton shell. J Bacteriol. 1991 Feb;173(3):1041–1050. doi: 10.1128/jb.173.3.1041-1050.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Stevens M. K., Krause D. C. Mycoplasma pneumoniae cytadherence phase-variable protein HMW3 is a component of the attachment organelle. J Bacteriol. 1992 Jul;174(13):4265–4274. doi: 10.1128/jb.174.13.4265-4274.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Su C. J., Chavoya A., Baseman J. B. Spontaneous mutation results in loss of the cytadhesin (P1) of Mycoplasma pneumoniae. Infect Immun. 1989 Oct;57(10):3237–3239. doi: 10.1128/iai.57.10.3237-3239.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Su C. J., Tryon V. V., Baseman J. B. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae. Infect Immun. 1987 Dec;55(12):3023–3029. doi: 10.1128/iai.55.12.3023-3029.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Wenzel R., Herrmann R. Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res. 1988 Sep 12;16(17):8323–8336. doi: 10.1093/nar/16.17.8323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Yayoshi M., Sasaki T., Yoshioka M. Relationship between an 85 kDa protein and the protective effects of Mycoplasma pneumoniae. Microbiol Immunol. 1992;36(5):455–464. doi: 10.1111/j.1348-0421.1992.tb02044.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES