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. 2003 Nov;62(Suppl 2):ii90–ii93. doi: 10.1136/ard.62.suppl_2.ii90

Prospects for the development of small molecular weight compounds to replace anti-tumour necrosis factor biological agents

B Foxwell, E Andreakos, F Brennan, M Feldmann, C Smith, M Conron
PMCID: PMC1766755  PMID: 14532159

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Figure 1.

Figure 1

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NIK is inessential for constitutive TNF production by primary rheumatoid synoviocytes (A) and sarcoid bronchoalveolar lavage (BAL) (B). Dissociated rheumatoid synoviocytes or cells from a BAL were uninfected or infected with an empty adenovirus (Ad0) or adenoviruses expressing NIK (AdNIK), or the kinase dead version of NIK (AdNIKkd) (multiplicity of infection (moi)=100 (A); moi=150 (B)). Cell supernatants were collected after 24 hours, and secreted TNF levels were determined by enzyme linked immunosorbent assay (ELISA). Data represent one experiment and are representative of three independent experiments using separate donors (SEM).

Figure 2.

Figure 2

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IKK2 has varying roles for constitutive TNF production by primary rheumatoid synoviocytes (A) and sarcoid BAL (B). Dissociated rheumatoid synoviocytes or cells from a BAL were uninfected (un) or infected with an empty adenovirus (Ad0) or adenoviruses expressing kinase dead version of IKK2 (IKK2dn), or IκBα (moi=100 (A); moi=150, (B)). Cell supernatants were collected after 24 hours, and secreted TNF levels were determined by ELISA. Data represent one experiment and are representative of three independent experiments using separate donors (SEM). The data are given as a percentage of TNF production by infected cells.

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