Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Mar;177(5):1380–1382. doi: 10.1128/jb.177.5.1380-1382.1995

Strain-dependent variation in carbon source regulation of nucleus-encoded mitochondrial proteins of Saccharomyces cerevisiae.

T A Brown 1, B L Trumpower 1
PMCID: PMC176746  PMID: 7868614

Abstract

Nuclear genes encoding mitochondrial proteins are regulated by carbon source with significant heterogeneity among four Saccharomyces cerevisiae strains. This strain-dependent variation is seen both in respiratory capacity of the cells and in the expression of beta-galactosidase reporter fusions to the promoters of CYB2, CYC1, CYC3, MnSOD, and RPO41.

Full Text

The Full Text of this article is available as a PDF (185.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borralho L. M., Malamud D. R., Panek A. D., Tenan M. N., Oliveira D. E., Mattoon J. R. Parallel changes in catabolite repression of haem biosynthesis and cytochromes in repression-resistant mutants of Saccharomyces cerevisiae. J Gen Microbiol. 1989 May;135(5):1217–1227. doi: 10.1099/00221287-135-5-1217. [DOI] [PubMed] [Google Scholar]
  2. Dumont M. E., Ernst J. F., Hampsey D. M., Sherman F. Identification and sequence of the gene encoding cytochrome c heme lyase in the yeast Saccharomyces cerevisiae. EMBO J. 1987 Jan;6(1):235–241. doi: 10.1002/j.1460-2075.1987.tb04744.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Entian K. D. Glucose repression: a complex regulatory system in yeast. Microbiol Sci. 1986 Dec;3(12):366–371. [PubMed] [Google Scholar]
  4. Fröhlich K. U., Entian K. D., Mecke D. The primary structure of the yeast hexokinase PII gene (HXK2) which is responsible for glucose repression. Gene. 1985;36(1-2):105–111. doi: 10.1016/0378-1119(85)90074-5. [DOI] [PubMed] [Google Scholar]
  5. Guarente L., Lalonde B., Gifford P., Alani E. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell. 1984 Feb;36(2):503–511. doi: 10.1016/0092-8674(84)90243-5. [DOI] [PubMed] [Google Scholar]
  6. Guarente L. Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol. 1983;101:181–191. doi: 10.1016/0076-6879(83)01013-7. [DOI] [PubMed] [Google Scholar]
  7. Guiard B. Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)-lactate cytochrome c oxidoreductase (cytochrome b2). EMBO J. 1985 Dec 1;4(12):3265–3272. doi: 10.1002/j.1460-2075.1985.tb04076.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kim K. S., Rosenkrantz M. S., Guarente L. Saccharomyces cerevisiae contains two functional citrate synthase genes. Mol Cell Biol. 1986 Jun;6(6):1936–1942. doi: 10.1128/mcb.6.6.1936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lodi T., Guiard B. Complex transcriptional regulation of the Saccharomyces cerevisiae CYB2 gene encoding cytochrome b2: CYP1(HAP1) activator binds to the CYB2 upstream activation site UAS1-B2. Mol Cell Biol. 1991 Jul;11(7):3762–3772. doi: 10.1128/mcb.11.7.3762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marres C. A., Van Loon A. P., Oudshoorn P., Van Steeg H., Grivell L. A., Slater E. C. Nucleotide sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein. Eur J Biochem. 1985 Feb 15;147(1):153–161. doi: 10.1111/j.1432-1033.1985.tb08731.x. [DOI] [PubMed] [Google Scholar]
  11. Masters B. S., Stohl L. L., Clayton D. A. Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7. Cell. 1987 Oct 9;51(1):89–99. doi: 10.1016/0092-8674(87)90013-4. [DOI] [PubMed] [Google Scholar]
  12. Myers A. M., Tzagoloff A., Kinney D. M., Lusty C. J. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene. 1986;45(3):299–310. doi: 10.1016/0378-1119(86)90028-4. [DOI] [PubMed] [Google Scholar]
  13. Perlman P. S., Mahler H. R. Derepression of mitochondria and their enzymes in yeast: regulatory aspects. Arch Biochem Biophys. 1974 May;162(1):248–271. doi: 10.1016/0003-9861(74)90125-8. [DOI] [PubMed] [Google Scholar]
  14. Rothstein R. J., Sherman F. Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions. Genetics. 1980 Apr;94(4):871–889. doi: 10.1093/genetics/94.4.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schüller H. J., Entian K. D. Extragenic suppressors of yeast glucose derepression mutants leading to constitutive synthesis of several glucose-repressible enzymes. J Bacteriol. 1991 Mar;173(6):2045–2052. doi: 10.1128/jb.173.6.2045-2052.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sikorski R. S., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989 May;122(1):19–27. doi: 10.1093/genetics/122.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith M., Leung D. W., Gillam S., Astell C. R., Montgomery D. L., Hall B. D. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae. Cell. 1979 Apr;16(4):753–761. doi: 10.1016/0092-8674(79)90091-6. [DOI] [PubMed] [Google Scholar]
  18. Szekely E., Montgomery D. L. Glucose represses transcription of Saccharomyces cerevisiae nuclear genes that encode mitochondrial components. Mol Cell Biol. 1984 May;4(5):939–946. doi: 10.1128/mcb.4.5.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Westerbeek-Marres C. A., Moore M. M., Autor A. P. Regulation of manganese superoxide dismutase in Saccharomyces cerevisiae. The role of respiratory chain activity. Eur J Biochem. 1988 Jul 1;174(4):611–620. doi: 10.1111/j.1432-1033.1988.tb14142.x. [DOI] [PubMed] [Google Scholar]
  20. Wilcoxen S. E., Peterson C. R., Winkley C. S., Keller M. J., Jaehning J. A. Two forms of RPO41-dependent RNA polymerase. Regulation of the RNA polymerase by glucose repression may control yeast mitochondrial gene expression. J Biol Chem. 1988 Sep 5;263(25):12346–12351. [PubMed] [Google Scholar]
  21. Wright R. M., Poyton R. O. Release of two Saccharomyces cerevisiae cytochrome genes, COX6 and CYC1, from glucose repression requires the SNF1 and SSN6 gene products. Mol Cell Biol. 1990 Mar;10(3):1297–1300. doi: 10.1128/mcb.10.3.1297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zimmermann F. K., Scheel I. Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Mol Gen Genet. 1977 Jul 7;154(1):75–82. doi: 10.1007/BF00265579. [DOI] [PubMed] [Google Scholar]
  23. Zitomer R. S., Montgomery D. L., Nichols D. L., Hall B. D. Transcriptional regulation of the yeast cytochrome c gene. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3627–3631. doi: 10.1073/pnas.76.8.3627. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES