Abstract
Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-point chromosomal comparison revealed only two inversions and a few translocations. Forces known to promote rearrangement, common in the haloarchaea, have been ineffective in changing global gene order throughout the nearly 10(7) years of these species' divergent evolution.
Full Text
The Full Text of this article is available as a PDF (222.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ben-Mahrez K., Sougakoff W., Nakayama M., Kohiyama M. Stimulation of an alpha like DNA polymerase by v-myc related protein of Halobacterium halobium. Arch Microbiol. 1988 Jan;149(3):175–180. doi: 10.1007/BF00422002. [DOI] [PubMed] [Google Scholar]
- Charlebois R. L., Hofman J. D., Schalkwyk L. C., Lam W. L., Doolittle W. F. Genome mapping in halobacteria. Can J Microbiol. 1989 Jan;35(1):21–29. doi: 10.1139/m89-004. [DOI] [PubMed] [Google Scholar]
- Charlebois R. L., Schalkwyk L. C., Hofman J. D., Doolittle W. F. Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2. J Mol Biol. 1991 Dec 5;222(3):509–524. doi: 10.1016/0022-2836(91)90493-p. [DOI] [PubMed] [Google Scholar]
- Cohen A., Lam W. L., Charlebois R. L., Doolittle W. F., Schalkwyk L. C. Localizing genes on the map of the genome of Haloferax volcanii, one of the Archaea. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1602–1606. doi: 10.1073/pnas.89.5.1602. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Conover R. K., Doolittle W. F. Characterization of a gene involved in histidine biosynthesis in Halobacterium (Haloferax) volcanii: isolation and rapid mapping by transformation of an auxotroph with cosmid DNA. J Bacteriol. 1990 Jun;172(6):3244–3249. doi: 10.1128/jb.172.6.3244-3249.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DasSarma S. Mechanisms of genetic variability in Halobacterium halobium: the purple membrane and gas vesicle mutations. Can J Microbiol. 1989 Jan;35(1):65–72. doi: 10.1139/m89-010. [DOI] [PubMed] [Google Scholar]
- Hackett N. R., Bobovnikova Y., Heyrovska N. Conservation of chromosomal arrangement among three strains of the genetically unstable archaeon Halobacterium salinarium. J Bacteriol. 1994 Dec;176(24):7711–7718. doi: 10.1128/jb.176.24.7711-7718.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmes M. L., Dyall-Smith M. L. Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria. J Bacteriol. 1991 Jan;173(2):642–648. doi: 10.1128/jb.173.2.642-648.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Itoh T. Complete nucleotide sequence of the ribosomal 'A' protein operon from the archaebacterium, Halobacterium halobium. Eur J Biochem. 1988 Sep 15;176(2):297–303. doi: 10.1111/j.1432-1033.1988.tb14281.x. [DOI] [PubMed] [Google Scholar]
- Kamekura M., Seno Y. Nucleotide sequences of 16S rRNA encoding genes from halophilic archaea Halococcus morrhuae NRC16008 and Haloferax mediterranei ATCC33500. Nucleic Acids Res. 1992 Jul 11;20(13):3517–3517. doi: 10.1093/nar/20.13.3517. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krawiec S., Riley M. Organization of the bacterial chromosome. Microbiol Rev. 1990 Dec;54(4):502–539. doi: 10.1128/mr.54.4.502-539.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lam W. L., Cohen A., Tsouluhas D., Doolittle W. F. Genes for tryptophan biosynthesis in the archaebacterium Haloferax volcanii. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6614–6618. doi: 10.1073/pnas.87.17.6614. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lam W. L., Doolittle W. F. Shuttle vectors for the archaebacterium Halobacterium volcanii. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5478–5482. doi: 10.1073/pnas.86.14.5478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lam W. L., Logan S. M., Doolittle W. F. Genes for tryptophan biosynthesis in the halophilic archaebacterium Haloferax volcanii: the trpDFEG cluster. J Bacteriol. 1992 Mar;174(5):1694–1697. doi: 10.1128/jb.174.5.1694-1697.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leffers H., Gropp F., Lottspeich F., Zillig W., Garrett R. A. Sequence, organization, transcription and evolution of RNA polymerase subunit genes from the archaebacterial extreme halophiles Halobacterium halobium and Halococcus morrhuae. J Mol Biol. 1989 Mar 5;206(1):1–17. doi: 10.1016/0022-2836(89)90519-6. [DOI] [PubMed] [Google Scholar]
- López-García P., Abad J. P., Smith C., Amils R. Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome. Nucleic Acids Res. 1992 May 25;20(10):2459–2464. doi: 10.1093/nar/20.10.2459. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ochman H., Wilson A. C. Evolution in bacteria: evidence for a universal substitution rate in cellular genomes. J Mol Evol. 1987;26(1-2):74–86. doi: 10.1007/BF02111283. [DOI] [PubMed] [Google Scholar]
- Pfeifer F., Betlach M. Genome organization in Halobacterium halobium: a 70 kb island of more (AT) rich DNA in the chromosome. Mol Gen Genet. 1985;198(3):449–455. doi: 10.1007/BF00332938. [DOI] [PubMed] [Google Scholar]
- Pfeifer F., Blaseio U., Horne M. Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants. Can J Microbiol. 1989 Jan;35(1):96–100. doi: 10.1139/m89-015. [DOI] [PubMed] [Google Scholar]
- Pfeifer F., Blaseio U. Transposition burst of the ISH27 insertion element family in Halobacterium halobium. Nucleic Acids Res. 1990 Dec 11;18(23):6921–6925. doi: 10.1093/nar/18.23.6921. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfeifer F., Weidinger G., Goebel W. Genetic variability in Halobacterium halobium. J Bacteriol. 1981 Jan;145(1):375–381. doi: 10.1128/jb.145.1.375-381.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenshine I., Mevarech M. Isolation and partial characterization of plasmids found in three Halobacterium volcanii isolates. Can J Microbiol. 1989 Jan;35(1):92–95. doi: 10.1139/m89-014. [DOI] [PubMed] [Google Scholar]
- Sapienza C., Doolittle W. F. Unusual physical organization of the Halobacterium genome. Nature. 1982 Feb 4;295(5848):384–389. doi: 10.1038/295384a0. [DOI] [PubMed] [Google Scholar]
- Sapienza C., Rose M. R., Doolittle W. F. High-frequency genomic rearrangements involving archaebacterial repeat sequence elements. Nature. 1982 Sep 9;299(5879):182–185. doi: 10.1038/299182a0. [DOI] [PubMed] [Google Scholar]
- Spiridonova V. A., Akhmanova A. S., Kagramanova V. K., Köpke A. K., Mankin A. S. Ribosomal protein gene cluster of Halobacterium halobium: nucleotide sequence of the genes coding for S3 and L29 equivalent ribosomal proteins. Can J Microbiol. 1989 Jan;35(1):153–159. doi: 10.1139/m89-023. [DOI] [PubMed] [Google Scholar]
- St Jean A., Trieselmann B. A., Charlebois R. L. Physical map and set of overlapping cosmid clones representing the genome of the archaeon Halobacterium sp. GRB. Nucleic Acids Res. 1994 Apr 25;22(8):1476–1483. doi: 10.1093/nar/22.8.1476. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vettakkorumakankav N. N., Stevenson K. J. Dihydrolipoamide dehydrogenase from Haloferax volcanii: gene cloning, complete primary structure, and comparison to other dihydrolipoamide dehydrogenases. Biochem Cell Biol. 1992 Aug;70(8):656–663. doi: 10.1139/o92-101. [DOI] [PubMed] [Google Scholar]
- Weidinger G., Klotz G., Goebel W. A large plasmid from Halobacterium halobium carrying genetic information for gas vacuole formation. Plasmid. 1979 Jul;2(3):377–386. doi: 10.1016/0147-619x(79)90021-0. [DOI] [PubMed] [Google Scholar]