Abstract
Clones expressing fragments of the flagellin protein of Salmonella enteritidis were constructed and screened with a g,m-specific monoclonal antibody. Results showed that the g,m epitope is localized between amino acids 258 and 348 of the flagellin. The fliC gene, encoding the flagellin of S. enteritidis, was proven to be the only flagellin gene present in S. enteritidis.
Full Text
The Full Text of this article is available as a PDF (233.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baay M. F., Huis in 't Veld J. H. Alternative antigens reduce cross-reactions in an ELISA for the detection of Salmonella enteritidis in poultry. J Appl Bacteriol. 1993 Mar;74(3):243–247. doi: 10.1111/j.1365-2672.1993.tb03021.x. [DOI] [PubMed] [Google Scholar]
- Beltran P., Musser J. M., Helmuth R., Farmer J. J., 3rd, Frerichs W. M., Wachsmuth I. K., Ferris K., McWhorter A. C., Wells J. G., Cravioto A. Toward a population genetic analysis of Salmonella: genetic diversity and relationships among strains of serotypes S. choleraesuis, S. derby, S. dublin, S. enteritidis, S. heidelberg, S. infantis, S. newport, and S. typhimurium. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7753–7757. doi: 10.1073/pnas.85.20.7753. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clouthier S. C., Müller K. H., Doran J. L., Collinson S. K., Kay W. W. Characterization of three fimbrial genes, sefABC, of Salmonella enteritidis. J Bacteriol. 1993 May;175(9):2523–2533. doi: 10.1128/jb.175.9.2523-2533.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galán J. E., Curtiss R., 3rd Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6383–6387. doi: 10.1073/pnas.86.16.6383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hyman H. C., Trachtenberg S. Point mutations that lock Salmonella typhimurium flagellar filaments in the straight right-handed and left-handed forms and their relation to filament superhelicity. J Mol Biol. 1991 Jul 5;220(1):79–88. doi: 10.1016/0022-2836(91)90382-g. [DOI] [PubMed] [Google Scholar]
- Joys T. M., Schödel F. Epitope mapping of the d flagellar antigen of Salmonella muenchen. Infect Immun. 1991 Sep;59(9):3330–3332. doi: 10.1128/iai.59.9.3330-3332.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kanto S., Okino H., Aizawa S., Yamaguchi S. Amino acids responsible for flagellar shape are distributed in terminal regions of flagellin. J Mol Biol. 1991 Jun 5;219(3):471–480. doi: 10.1016/0022-2836(91)90187-b. [DOI] [PubMed] [Google Scholar]
- Kusters J. G., Jager E. J., van der Zeijst B. A. Improvement of the cloning linker of the bacterial expression vector pEX. Nucleic Acids Res. 1989 Oct 11;17(19):8007–8007. doi: 10.1093/nar/17.19.8007. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masten B. J., Joys T. M. Molecular analyses of the Salmonella g. . . flagellar antigen complex. J Bacteriol. 1993 Sep;175(17):5359–5365. doi: 10.1128/jb.175.17.5359-5365.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Namba K., Yamashita I., Vonderviszt F. Structure of the core and central channel of bacterial flagella. Nature. 1989 Dec 7;342(6250):648–654. doi: 10.1038/342648a0. [DOI] [PubMed] [Google Scholar]
- Newton S. M., Jacob C. O., Stocker B. A. Immune response to cholera toxin epitope inserted in Salmonella flagellin. Science. 1989 Apr 7;244(4900):70–72. doi: 10.1126/science.2468182. [DOI] [PubMed] [Google Scholar]
- Newton S. M., Kotb M., Poirier T. P., Stocker B. A., Beachey E. H. Expression and immunogenicity of a streptococcal M protein epitope inserted in Salmonella flagellin. Infect Immun. 1991 Jun;59(6):2158–2165. doi: 10.1128/iai.59.6.2158-2165.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newton S. M., Wasley R. D., Wilson A., Rosenberg L. T., Miller J. F., Stocker B. A. Segment IV of a Salmonella flagellin gene specifies flagellar antigen epitopes. Mol Microbiol. 1991 Feb;5(2):419–425. doi: 10.1111/j.1365-2958.1991.tb02124.x. [DOI] [PubMed] [Google Scholar]
- Petter J. G. Detection of two smooth colony phenotypes in a Salmonella enteritidis isolate which vary in their ability to contaminate eggs. Appl Environ Microbiol. 1993 Sep;59(9):2884–2890. doi: 10.1128/aem.59.9.2884-2890.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Popoff M. Y., Bockemühl J., McWhorter-Murlin A. Supplement 1990 (no. 34) to the Kauffmann-White scheme. Res Microbiol. 1991 Nov-Dec;142(9):1029–1033. doi: 10.1016/0923-2508(91)90014-2. [DOI] [PubMed] [Google Scholar]
- Rodrigue D. C., Tauxe R. V., Rowe B. International increase in Salmonella enteritidis: a new pandemic? Epidemiol Infect. 1990 Aug;105(1):21–27. doi: 10.1017/s0950268800047609. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selander R. K., Smith N. H., Li J., Beltran P., Ferris K. E., Kopecko D. J., Rubin F. A. Molecular evolutionary genetics of the cattle-adapted serovar Salmonella dublin. J Bacteriol. 1992 Jun;174(11):3587–3592. doi: 10.1128/jb.174.11.3587-3592.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- St Louis M. E., Morse D. L., Potter M. E., DeMelfi T. M., Guzewich J. J., Tauxe R. V., Blake P. A. The emergence of grade A eggs as a major source of Salmonella enteritidis infections. New implications for the control of salmonellosis. JAMA. 1988 Apr 8;259(14):2103–2107. [PubMed] [Google Scholar]
- Stone B. J., Garcia C. M., Badger J. L., Hassett T., Smith R. I., Miller V. L. Identification of novel loci affecting entry of Salmonella enteritidis into eukaryotic cells. J Bacteriol. 1992 Jun;174(12):3945–3952. doi: 10.1128/jb.174.12.3945-3952.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorns C. J., Sojka M. G., Chasey D. Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody. J Clin Microbiol. 1990 Nov;28(11):2409–2414. doi: 10.1128/jcm.28.11.2409-2414.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turcotte C., Woodward M. J. Cloning, DNA nucleotide sequence and distribution of the gene encoding the SEF14 fimbrial antigen of Salmonella enteritidis. J Gen Microbiol. 1993 Jul;139(7):1477–1485. doi: 10.1099/00221287-139-7-1477. [DOI] [PubMed] [Google Scholar]
- Wei L. N., Joys T. M. Covalent structure of three phase-1 flagellar filament proteins of Salmonella. J Mol Biol. 1985 Dec 20;186(4):791–803. doi: 10.1016/0022-2836(85)90397-3. [DOI] [PubMed] [Google Scholar]
- van Vliet A. H., Jongejan F., van der Zeijst B. A. Phylogenetic position of Cowdria ruminantium (Rickettsiales) determined by analysis of amplified 16S ribosomal DNA sequences. Int J Syst Bacteriol. 1992 Jul;42(3):494–498. doi: 10.1099/00207713-42-3-494. [DOI] [PubMed] [Google Scholar]
- van Zijderveld F. G., van Zijderveld-van Bemmel A. M., Anakotta J. Comparison of four different enzyme-linked immunosorbent assays for serological diagnosis of Salmonella enteritidis infections in experimentally infected chickens. J Clin Microbiol. 1992 Oct;30(10):2560–2566. doi: 10.1128/jcm.30.10.2560-2566.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]