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. 1995 Apr;177(8):1976–1980. doi: 10.1128/jb.177.8.1976-1980.1995

Protein shift and antigenic variation in the S-layer of Campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapA homologs.

M M Garcia 1, C L Lutze-Wallace 1, A S Denes 1, M D Eaglesome 1, E Holst 1, M J Blaser 1
PMCID: PMC176838  PMID: 7721688

Abstract

Campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a C. fetus-negative heifer. Isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kDa S-layer protein. Immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kDa S-layer protein and other C. fetus S-layer proteins demonstrated antigenic shifts. Genomic digests of the isolates probed with a 75-mer oligonucleotide of the conserved sapA region also indicated that antigenic variation of the S-layer is accompanied by DNA rearrangement.

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Selected References

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  1. Blaser M. J., Gotschlich E. C. Surface array protein of Campylobacter fetus. Cloning and gene structure. J Biol Chem. 1990 Aug 25;265(24):14529–14535. [PubMed] [Google Scholar]
  2. Blaser M. J., Hopkins J. A., Berka R. M., Vasil M. L., Wang W. L. Identification and characterization of Campylobacter jejuni outer membrane proteins. Infect Immun. 1983 Oct;42(1):276–284. doi: 10.1128/iai.42.1.276-284.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brooks B. W., Garcia M. M., Fraser D. E., Lior H., Stewart R. B., Lammerding A. M. Isolation and characterization of cephalothin-susceptible Campylobacter coli from slaughter cattle. J Clin Microbiol. 1986 Oct;24(4):591–595. doi: 10.1128/jcm.24.4.591-595.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Corbeil L. B., Duncan J. R., Schurig G. G., Hall C. E., Winter A. J. Bovine venereal vibriosis: variations in immunoglobulin class of antibodies in genital secretions and serum. Infect Immun. 1974 Nov;10(5):1084–1090. doi: 10.1128/iai.10.5.1084-1090.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Corbeil L. B., Schurig G. G., Bier P. J., Winter A. J. Bovine veneral vibriosis: antigenic variation of the bacterium during infection. Infect Immun. 1975 Feb;11(2):240–244. doi: 10.1128/iai.11.2.240-244.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubreuil J. D., Kostrzynska M., Austin J. W., Trust T. J. Antigenic differences among Campylobacter fetus S-layer proteins. J Bacteriol. 1990 Sep;172(9):5035–5043. doi: 10.1128/jb.172.9.5035-5043.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dubreuil J. D., Logan S. M., Cubbage S., Eidhin D. N., McCubbin W. D., Kay C. M., Beveridge T. J., Ferris F. G., Trust T. J. Structural and biochemical analyses of a surface array protein of Campylobacter fetus. J Bacteriol. 1988 Sep;170(9):4165–4173. doi: 10.1128/jb.170.9.4165-4173.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fuerst J. A., Perry J. W. Demonstration of lipopolysaccharide on sheathed flagella of Vibrio cholerae O:1 by protein A-gold immunoelectron microscopy. J Bacteriol. 1988 Apr;170(4):1488–1494. doi: 10.1128/jb.170.4.1488-1494.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fujimoto S., Takade A., Amako K., Blaser M. J. Correlation between molecular size of the surface array protein and morphology and antigenicity of the Campylobacter fetus S layer. Infect Immun. 1991 Jun;59(6):2017–2022. doi: 10.1128/iai.59.6.2017-2022.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Garcia M. M., Becker S. A., Brooks B. W., Berg J. N., Finegold S. M. Ultrastructure and molecular characterization of Fusobacterium necrophorum biovars. Can J Vet Res. 1992 Oct;56(4):318–325. [PMC free article] [PubMed] [Google Scholar]
  11. Holst E., Wathne B., Hovelius B., Mårdh P. A. Bacterial vaginosis: microbiological and clinical findings. Eur J Clin Microbiol. 1987 Oct;6(5):536–541. doi: 10.1007/BF02014242. [DOI] [PubMed] [Google Scholar]
  12. Kennett R. H., Denis K. A., Tung A. S., Klinman N. R. Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells. Curr Top Microbiol Immunol. 1978;81:77–91. doi: 10.1007/978-3-642-67448-8_13. [DOI] [PubMed] [Google Scholar]
  13. Logan S. M., Trust T. J. Outer membrane characteristics of Campylobacter jejuni. Infect Immun. 1982 Dec;38(3):898–906. doi: 10.1128/iai.38.3.898-906.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murray M. G., Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980 Oct 10;8(19):4321–4325. doi: 10.1093/nar/8.19.4321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pei Z., Blaser M. J. Pathogenesis of Campylobacter fetus infections. Role of surface array proteins in virulence in a mouse model. J Clin Invest. 1990 Apr;85(4):1036–1043. doi: 10.1172/JCI114533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pei Z., Ellison R. T., 3rd, Lewis R. V., Blaser M. J. Purification and characterization of a family of high molecular weight surface-array proteins from Campylobacter fetus. J Biol Chem. 1988 May 5;263(13):6416–6420. [PubMed] [Google Scholar]
  17. Perez-Perez G. I., Blaser M. J., Bryner J. H. Lipopolysaccharide structures of Campylobacter fetus are related to heat-stable serogroups. Infect Immun. 1986 Jan;51(1):209–212. doi: 10.21236/ada265573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Salama S. M., Garcia M. M., Taylor D. E. Differentiation of the subspecies of Campylobacter fetus by genomic sizing. Int J Syst Bacteriol. 1992 Jul;42(3):446–450. doi: 10.1099/00207713-42-3-446. [DOI] [PubMed] [Google Scholar]
  19. Salvi R. J., Ahroon W., Saunders S. S., Arnold S. A. Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion. Ear Hear. 1987 Jun;8(3):151–156. [PubMed] [Google Scholar]
  20. Schurig G. D., Hall C. E., Burda K., Corbeil L. B., Duncan J. R., Winter A. J. Persistent genital tract infection with Vibrio fetus intestinalis associated with serotypic alteration of the infecting strain. Am J Vet Res. 1973 Nov;34(11):1399–1403. [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tummuru M. K., Blaser M. J. Characterization of the Campylobacter fetus sapA promoter: evidence that the sapA promoter is deleted in spontaneous mutant strains. J Bacteriol. 1992 Sep;174(18):5916–5922. doi: 10.1128/jb.174.18.5916-5922.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tummuru M. K., Blaser M. J. Rearrangement of sapA homologs with conserved and variable regions in Campylobacter fetus. Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7265–7269. doi: 10.1073/pnas.90.15.7265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wang E., Garcia M. M., Blake M. S., Pei Z., Blaser M. J. Shift in S-layer protein expression responsible for antigenic variation in Campylobacter fetus. J Bacteriol. 1993 Aug;175(16):4979–4984. doi: 10.1128/jb.175.16.4979-4984.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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