Abstract
Previously, the repeating-unit structure of the S-layer glycoprotein from the eubacterium Bacillus alvei CCM 2051 has been determined to be [-->3)-beta-D-Galp-(1-->4)-[alpha-D-Glcp-(1-->6)-]-beta-D-ManpNAc- (1-->]n (E. Altman, J.-R. Brisson, P. Messner, and U. B. Sleytr, Biochem. Cell Biol. 69:72-78, 1991). Nuclear magnetic resonance spectroscopic reexamination of this glycan reveals that the O-antigen-like domain of the polysaccharide is [see text] connected with the S-layer polypeptide through the "core" structure -->3)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap-(1-->3)-alpha-L-R hap-(1-->3)-beta-D-Galp-(1-->O)-Tyr. Except for the substitution in position 4 of the nonreducing rhamnose with the modified glyceric acid phosphate residue GroA-2-->OPO2-->4-beta-D-ManpNAc-(1-->, this core is identical to the core of the tyrosine-linked glycan from the S-layer glycoprotein of Thermoanaerobacter thermohydrosulfuricus L111-69 (K. Bock, J. Schuster-Kolbe, E. Altman, G. Allmaier, B. Stahl, R. Christian, U. B. Sleytr, and P. Messner, J. Biol. Chem. 269:7137-7144, 1994).
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- Altman E., Brisson J. R., Messner P., Sleytr U. B. Structure of the glycan chain from the surface layer glycoprotein of Bacillus alvei CCM 2051. Biochem Cell Biol. 1991 Jan;69(1):72–78. doi: 10.1139/o91-010. [DOI] [PubMed] [Google Scholar]
- Bock K., Schuster-Kolbe J., Altman E., Allmaier G., Stahl B., Christian R., Sleytr U. B., Messner P. Primary structure of the O-glycosidically linked glycan chain of the crystalline surface layer glycoprotein of Thermoanaerobacter thermohydrosulfuricus L111-69. Galactosyl tyrosine as a novel linkage unit. J Biol Chem. 1994 Mar 11;269(10):7137–7144. [PubMed] [Google Scholar]
- Christian R., Messner P., Weiner C., Sleytr U. B., Schulz G. Structure of a glycan from the surface-layer glycoprotein of Clostridium thermohydrosulfuricum strain L111-69. Carbohydr Res. 1988 May 1;176(1):160–163. doi: 10.1016/0008-6215(88)84069-2. [DOI] [PubMed] [Google Scholar]
- Cox A. D., Wilkinson S. G. Structures of the O-specific polymers from the lipopolysaccharides of the reference strains for Pseudomonas cepacia serogroups O3 and O5. Carbohydr Res. 1989 Dec 21;195(1):123–129. doi: 10.1016/0008-6215(89)85095-5. [DOI] [PubMed] [Google Scholar]
- Knirel' I. U., Kocharova N. A., Shashkov A. S., Varbanets L. D., Kochetkov N. K. Antigennye polisakharidy bakterii. 17. Struktura O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Pseudomonas aeruginosa X (Meitert). Bioorg Khim. 1986 Sep;12(9):1268–1273. [PubMed] [Google Scholar]
- Kärcher U., Schröder H., Haslinger E., Allmaier G., Schreiner R., Wieland F., Haselbeck A., König H. Primary structure of the heterosaccharide of the surface glycoprotein of Methanothermus fervidus. J Biol Chem. 1993 Dec 25;268(36):26821–26826. [PubMed] [Google Scholar]
- LOWRY O. H., ROBERTS N. R., LEINER K. Y., WU M. L., FARR A. L. The quantitative histochemistry of brain. I. Chemical methods. J Biol Chem. 1954 Mar;207(1):1–17. [PubMed] [Google Scholar]
- Lechner J., Wieland F. Structure and biosynthesis of prokaryotic glycoproteins. Annu Rev Biochem. 1989;58:173–194. doi: 10.1146/annurev.bi.58.070189.001133. [DOI] [PubMed] [Google Scholar]
- Lipkind G. M., Shashkov A. S., Nifant'ev N. E., Kochetkov N. K. Computer-assisted analysis of the structure of regular branched polysaccharides containing 2,3-disubstituted rhamnopyranose and mannopyranose residues on the basis of 13C NMR data. Carbohydr Res. 1992 Dec 31;237:11–22. doi: 10.1016/s0008-6215(92)84229-l. [DOI] [PubMed] [Google Scholar]
- Messner P., Christian R., Kolbe J., Schulz G., Sleytr U. B. Analysis of a novel linkage unit of O-linked carbohydrates from the crystalline surface layer glycoprotein of Clostridium thermohydrosulfuricum S102-70. J Bacteriol. 1992 Apr;174(7):2236–2240. doi: 10.1128/jb.174.7.2236-2240.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Messner P., Sleytr U. B. Bacterial surface layer glycoproteins. Glycobiology. 1991 Dec;1(6):545–551. doi: 10.1093/glycob/1.6.545. [DOI] [PubMed] [Google Scholar]
- Messner P., Sleytr U. B. Crystalline bacterial cell-surface layers. Adv Microb Physiol. 1992;33:213–275. doi: 10.1016/s0065-2911(08)60218-0. [DOI] [PubMed] [Google Scholar]