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. 1995 Apr;177(8):2209–2213. doi: 10.1128/jb.177.8.2209-2213.1995

A stationary-phase-dependent viability block governed by two different polypeptides from the RhsA genetic element of Escherichia coli K-12.

D A Vlazny 1, C W Hill 1
PMCID: PMC176868  PMID: 7721712

Abstract

Multicopy plasmids bearing a small internal portion of the RhsA genetic element of Escherichia coli K-12 imparted a viability block on cultures grown to stationary phase in broth. Inclusion of the last 25 codons of the RhsA core open reading frame (called core-ORF) in the plasmid insert was crucial for eliciting this toxic effect. The toxic effect could be suppressed by including the adjacent Rhs component, dsORF-a1, on the multicopy plasmid. The toxic effect was enhanced in RpoS- strains.

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Selected References

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