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. 1995 Apr;177(8):2241–2244. doi: 10.1128/jb.177.8.2241-2244.1995

Carbon monoxide-dependent growth of Rhodospirillum rubrum.

R L Kerby 1, P W Ludden 1, G P Roberts 1
PMCID: PMC176875  PMID: 7721719

Abstract

Under dark, anaerobic conditions in the presence of sufficient nickel, Rhodospirillum rubrum grows with a doubling time of under 5 h by coupling the oxidation of CO to the reduction of H+ to H2. CO-dependent growth of R. rubrum UR294, bearing a kanamycin resistance cassette in cooC, depends on a medium nickel level ninefold higher than that required for optimal growth of coo+ strains.

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Selected References

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  1. Bott M., Eikmanns B., Thauer R. K. Coupling of carbon monoxide oxidation to CO2 and H2 with the phosphorylation of ADP in acetate-grown Methanosarcina barkeri. Eur J Biochem. 1986 Sep 1;159(2):393–398. doi: 10.1111/j.1432-1033.1986.tb09881.x. [DOI] [PubMed] [Google Scholar]
  2. Bott M., Thauer R. K. Proton translocation coupled to the oxidation of carbon monoxide to CO2 and H2 in Methanosarcina barkeri. Eur J Biochem. 1989 Feb 1;179(2):469–472. doi: 10.1111/j.1432-1033.1989.tb14576.x. [DOI] [PubMed] [Google Scholar]
  3. Champine J. E., Uffen R. L. Membrane topography of anaerobic carbon monoxide oxidation in Rhodocyclus gelatinosus. J Bacteriol. 1987 Oct;169(10):4784–4789. doi: 10.1128/jb.169.10.4784-4789.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daniel S. L., Hsu T., Dean S. I., Drake H. L. Characterization of the H2- and CO-dependent chemolithotrophic potentials of the acetogens Clostridium thermoaceticum and Acetogenium kivui. J Bacteriol. 1990 Aug;172(8):4464–4471. doi: 10.1128/jb.172.8.4464-4471.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dernedde J., Eitinger M., Friedrich B. Analysis of a pleiotropic gene region involved in formation of catalytically active hydrogenases in Alcaligenes eutrophus H16. Arch Microbiol. 1993;159(6):545–553. doi: 10.1007/BF00249034. [DOI] [PubMed] [Google Scholar]
  6. Dimroth P. The ATPases of Propionigenium modestum and Bacillus alcalophilus. Strategies for ATP synthesis under low energy conditions. Biochim Biophys Acta. 1992 Jul 17;1101(2):236–239. [PubMed] [Google Scholar]
  7. Ensign S. A., Bonam D., Ludden P. W. Nickel is required for the transfer of electrons from carbon monoxide to the iron-sulfur center(s) of carbon monoxide dehydrogenase from Rhodospirillum rubrum. Biochemistry. 1989 Jun 13;28(12):4968–4973. doi: 10.1021/bi00438a010. [DOI] [PubMed] [Google Scholar]
  8. Ensign S. A., Ludden P. W. Characterization of the CO oxidation/H2 evolution system of Rhodospirillum rubrum. Role of a 22-kDa iron-sulfur protein in mediating electron transfer between carbon monoxide dehydrogenase and hydrogenase. J Biol Chem. 1991 Sep 25;266(27):18395–18403. [PubMed] [Google Scholar]
  9. Falk G., Walker J. E. DNA sequence of a gene cluster coding for subunits of the F0 membrane sector of ATP synthase in Rhodospirillum rubrum. Support for modular evolution of the F1 and F0 sectors. Biochem J. 1988 Aug 15;254(1):109–122. doi: 10.1042/bj2540109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ferry J. G. Methane from acetate. J Bacteriol. 1992 Sep;174(17):5489–5495. doi: 10.1128/jb.174.17.5489-5495.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fu C., Maier R. J. Nucleotide sequences of two hydrogenase-related genes (hypA and hypB) from Bradyrhizobium japonicum, one of which (hypB) encodes an extremely histidine-rich region and guanine nucleotide-binding domains. Biochim Biophys Acta. 1994 Feb 8;1184(1):135–138. doi: 10.1016/0005-2728(94)90163-5. [DOI] [PubMed] [Google Scholar]
  12. Hirsch P. Photosynthetic bacterium growing under carbon monoxide. Nature. 1968 Feb 10;217(5128):555–556. doi: 10.1038/217555a0. [DOI] [PubMed] [Google Scholar]
  13. Kerby R. L., Hong S. S., Ensign S. A., Coppoc L. J., Ludden P. W., Roberts G. P. Genetic and physiological characterization of the Rhodospirillum rubrum carbon monoxide dehydrogenase system. J Bacteriol. 1992 Aug;174(16):5284–5294. doi: 10.1128/jb.174.16.5284-5294.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee M. H., Mulrooney S. B., Renner M. J., Markowicz Y., Hausinger R. P. Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis. J Bacteriol. 1992 Jul;174(13):4324–4330. doi: 10.1128/jb.174.13.4324-4330.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ljungdahl L. G. The autotrophic pathway of acetate synthesis in acetogenic bacteria. Annu Rev Microbiol. 1986;40:415–450. doi: 10.1146/annurev.mi.40.100186.002215. [DOI] [PubMed] [Google Scholar]
  16. Maier T., Jacobi A., Sauter M., Böck A. The product of the hypB gene, which is required for nickel incorporation into hydrogenases, is a novel guanine nucleotide-binding protein. J Bacteriol. 1993 Feb;175(3):630–635. doi: 10.1128/jb.175.3.630-635.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. O'Brien J. M., Wolkin R. H., Moench T. T., Morgan J. B., Zeikus J. G. Association of hydrogen metabolism with unitrophic or mixotrophic growth of Methanosarcina barkeri on carbon monoxide. J Bacteriol. 1984 Apr;158(1):373–375. doi: 10.1128/jb.158.1.373-375.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schultz J. E., Weaver P. F. Fermentation and anaerobic respiration by Rhodospirillum rubrum and Rhodopseudomonas capsulata. J Bacteriol. 1982 Jan;149(1):181–190. doi: 10.1128/jb.149.1.181-190.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Terlesky K. C., Ferry J. G. Ferredoxin requirement for electron transport from the carbon monoxide dehydrogenase complex to a membrane-bound hydrogenase in acetate-grown Methanosarcina thermophila. J Biol Chem. 1988 Mar 25;263(9):4075–4079. [PubMed] [Google Scholar]
  20. Thauer R. K., Möller-Zinkhan D., Spormann A. M. Biochemistry of acetate catabolism in anaerobic chemotrophic bacteria. Annu Rev Microbiol. 1989;43:43–67. doi: 10.1146/annurev.mi.43.100189.000355. [DOI] [PubMed] [Google Scholar]
  21. Uffen R. L. Anaerobic growth of a Rhodopseudomonas species in the dark with carbon monoxide as sole carbon and energy substrate. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3298–3302. doi: 10.1073/pnas.73.9.3298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Uffen R. L. Metabolism of carbon monoxide by Rhodopseudomonas gelatinosa: cell growth and properties of the oxidation system. J Bacteriol. 1983 Sep;155(3):956–965. doi: 10.1128/jb.155.3.956-965.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wood P. M. The redox potential for dimethyl sulphoxide reduction to dimethyl sulphide: evaluation and biochemical implications. FEBS Lett. 1981 Feb 9;124(1):11–14. doi: 10.1016/0014-5793(81)80042-7. [DOI] [PubMed] [Google Scholar]

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