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. 1995 May;177(9):2589–2591. doi: 10.1128/jb.177.9.2589-2591.1995

Structural and functional analyses of the transcription-translation proteins NusB and NusE.

D L Court 1, T A Patterson 1, T Baker 1, N Costantino 1, X Mao 1, D I Friedman 1
PMCID: PMC176924  PMID: 7730297

Abstract

The NusB and NusE (ribosomal protein S10) proteins function in transcription and translation. The two proteins form a complex that binds to the boxA sequence found in the leader RNA of rrn operons; boxA is required for transcription antitermination in rrn operons. Although binding of these two proteins to the boxA RNA of the bacteriophage lambda nut site has not been observed, both NusB and NusE as well as the RNA boxA sequence are required for lambda N-mediated antitermination. Studies identifying the amino acid changes caused by mutations in nusB and nusE and relating these changes to altered function are reported. It is concluded that boxA is essential for an effective NusB contribution to N-mediated antitermination and that by mutation NusB may be changed to allow more-effective binding to boxA variants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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