Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 May;177(10):2933–2937. doi: 10.1128/jb.177.10.2933-2937.1995

Genes that protect against the host-killing activity of the E3 protein of Bacillus subtilis bacteriophage SPO1.

P Wei 1, C R Stewart 1
PMCID: PMC176973  PMID: 7751311

Abstract

A cloned rpoB gene, specifying an apparently mutant RNA polymerase beta subunit, protected Escherichia coli against the cytocidal effects of the E3 protein of bacteriophage SPO1, suggesting that RNA polymerase is the primary cellular target of the E3 protein. Two segments of the wild-type E. coli genome, one of which specifies a suppressor of dnaK mutations, and thus, possibly, a molecular chaperone, also provided protection when overexpressed, but wild-type rpoB did not.

Full Text

The Full Text of this article is available as a PDF (276.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernard P., Couturier M. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J Mol Biol. 1992 Aug 5;226(3):735–745. doi: 10.1016/0022-2836(92)90629-x. [DOI] [PubMed] [Google Scholar]
  2. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chauhan A. K., Apirion D. The gene for a small stable RNA (10Sa RNA) of Escherichia coli. Mol Microbiol. 1989 Nov;3(11):1481–1485. doi: 10.1111/j.1365-2958.1989.tb00133.x. [DOI] [PubMed] [Google Scholar]
  4. Greer H. Host mutants resistant to phage lambda killing. Virology. 1975 Aug;66(2):605–609. doi: 10.1016/0042-6822(75)90232-9. [DOI] [PubMed] [Google Scholar]
  5. Hanahan D., Jessee J., Bloom F. R. Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol. 1991;204:63–113. doi: 10.1016/0076-6879(91)04006-a. [DOI] [PubMed] [Google Scholar]
  6. Kang P. J., Craig E. A. Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation. J Bacteriol. 1990 Apr;172(4):2055–2064. doi: 10.1128/jb.172.4.2055-2064.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kashlev M., Nudler E., Goldfarb A., White T., Kutter E. Bacteriophage T4 Alc protein: a transcription termination factor sensing local modification of DNA. Cell. 1993 Oct 8;75(1):147–154. [PubMed] [Google Scholar]
  8. Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y. L., Komano T. Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli. J Bacteriol. 1991 Apr;173(8):2644–2648. doi: 10.1128/jb.173.8.2644-2648.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kirby J. E., Trempy J. E., Gottesman S. Excision of a P4-like cryptic prophage leads to Alp protease expression in Escherichia coli. J Bacteriol. 1994 Apr;176(7):2068–2081. doi: 10.1128/jb.176.7.2068-2081.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Landick R., Colwell A., Stewart J. Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase. J Bacteriol. 1990 Jun;172(6):2844–2854. doi: 10.1128/jb.172.6.2844-2854.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maiti S., Das B., Mandal N. C. Isolation and preliminary characterization of Escherichia coli mutants resistant to lethal action of the bacteriophage lambda P gene. Virology. 1991 May;182(1):351–352. doi: 10.1016/0042-6822(91)90679-6. [DOI] [PubMed] [Google Scholar]
  12. Miczak A., Chauhan A. K., Apirion D. Two new genes located between 2758 and 2761 kilobase pairs on the Escherichia coli genome. J Bacteriol. 1991 Jun;173(11):3271–3272. doi: 10.1128/jb.173.11.3271-3272.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miki T., Orita T., Furuno M., Horiuchi T. Control of cell division by sex factor F in Escherichia coli. III. Participation of the groES (mopB) gene of the host bacteria. J Mol Biol. 1988 May 20;201(2):327–338. doi: 10.1016/0022-2836(88)90141-6. [DOI] [PubMed] [Google Scholar]
  14. Miki T., Park J. A., Nagao K., Murayama N., Horiuchi T. Control of segregation of chromosomal DNA by sex factor F in Escherichia coli. Mutants of DNA gyrase subunit A suppress letD (ccdB) product growth inhibition. J Mol Biol. 1992 May 5;225(1):39–52. doi: 10.1016/0022-2836(92)91024-j. [DOI] [PubMed] [Google Scholar]
  15. Ovchinnikov Y. A., Monastyrskaya G. S., Gubanov V. V., Guryev S. O., Chertov OYu, Modyanov N. N., Grinkevich V. A., Makarova I. A., Marchenko T. V., Polovnikova I. N. The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit. Eur J Biochem. 1981 Jun 1;116(3):621–629. doi: 10.1111/j.1432-1033.1981.tb05381.x. [DOI] [PubMed] [Google Scholar]
  16. Poulsen L. K., Larsen N. W., Molin S., Andersson P. Analysis of an Escherichia coli mutant strain resistant to the cell-killing function encoded by the gef gene family. Mol Microbiol. 1992 Apr;6(7):895–905. doi: 10.1111/j.1365-2958.1992.tb01540.x. [DOI] [PubMed] [Google Scholar]
  17. Retallack D. M., Johnson L. L., Friedman D. I. Role for 10Sa RNA in the growth of lambda-P22 hybrid phage. J Bacteriol. 1994 Apr;176(7):2082–2089. doi: 10.1128/jb.176.7.2082-2089.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rose R. E. The nucleotide sequence of pACYC177. Nucleic Acids Res. 1988 Jan 11;16(1):356–356. doi: 10.1093/nar/16.1.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Severinov K., Kashlev M., Severinova E., Bass I., McWilliams K., Kutter E., Nikiforov V., Snyder L., Goldfarb A. A non-essential domain of Escherichia coli RNA polymerase required for the action of the termination factor Alc. J Biol Chem. 1994 May 13;269(19):14254–14259. [PubMed] [Google Scholar]
  20. Skowyra D., Georgopoulos C., Zylicz M. The E. coli dnaK gene product, the hsp70 homolog, can reactivate heat-inactivated RNA polymerase in an ATP hydrolysis-dependent manner. Cell. 1990 Sep 7;62(5):939–944. doi: 10.1016/0092-8674(90)90268-j. [DOI] [PubMed] [Google Scholar]
  21. Snyder L., Jorissen L. Escherichia coli mutations that prevent the action of the T4 unf/alc protein map in an RNA polymerase gene. Genetics. 1988 Feb;118(2):173–180. doi: 10.1093/genetics/118.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tintut Y., Wong C., Jiang Y., Hsieh M., Gralla J. D. RNA polymerase binding using a strongly acidic hydrophobic-repeat region of sigma 54. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2120–2124. doi: 10.1073/pnas.91.6.2120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wei P., Stewart C. R. A cytotoxic early gene of Bacillus subtilis bacteriophage SPO1. J Bacteriol. 1993 Dec;175(24):7887–7900. doi: 10.1128/jb.175.24.7887-7900.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES