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. 1995 Jun;177(11):3308–3311. doi: 10.1128/jb.177.11.3308-3311.1995

Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis.

S J Vidwans 1, K Ireton 1, A D Grossman 1
PMCID: PMC177024  PMID: 7768831

Abstract

We fused obg, encoding an essential GTP-binding protein in Bacillus subtilis, to the LacI-repressible, IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter Pspac. Depletion of Obg, following removal of IPTG, caused a defect in sporulation and in expression of sporulation genes that are activated by Spo0A approximately P. These defects were significantly relieved by a mutation in spo0A (rvtA11) that bypasses the normal phosphorylation pathway, indicating that Obg might normally be required, either directly or indirectly, to stimulate activity of the phosphorelay that activates Spo0A.

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Selected References

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